轮作模式和氮肥处理对稻田土壤有机碳储量及其结构的影响

doi:10.16819/j.1001-7216.2024.230912

中国水稻科学 ›› 2024, Vol. 38 ›› Issue (5): 577-590.DOI: 10.16819/j.1001-7216.2024.230912

• 研究报告 • 上一篇

轮作模式和氮肥处理对稻田土壤有机碳储量及其结构的影响

周子榆¹, 王孟佳¹^,², 冯向前¹^,³, 覃金华¹^,³, 王爱冬¹, 马横宇¹, 褚光¹, 刘元辉¹, 徐春梅¹, 章秀福¹, 王丹英¹, 郑希⁴^,^*(), 陈松¹^,^*()

¹中国水稻研究所，杭州 311401
²滨州中裕农业科学研究院，山东滨州 256600
³长江大学农学院，湖北荆州 434025
⁴浙江大学电子显微镜中心生命科学分部，杭州 310058

收稿日期:2023-09-25 修回日期:2024-01-16 出版日期:2024-09-10 发布日期:2024-09-10
通讯作者: *email: xzheng@zju.edu.cn; chensong02@caas.cn
基金资助:
国家自然科学基金资助项目(32172106);浙江省自然科学基金资助项目(LY22C130001);国家水稻产业技术体系项目(CARS-01);中国农业科学院科技创新工程重大科研任务资助项目(CAAS-ZDRW202001);浙江省“万人计划”科技创新领军人才项目(2020R52035);中国农业科学院科技创新工程计划资助项目;中央级公益性科研院所基本科研业务费专项(CPSIBRF-CNRRI-202119)

Effects of Crop Rotation Patterns and Nitrogen Fertilizer Levels on Storage and Structure of Soil Organic Carbon in Paddy Fields

ZHOU Ziyu¹, WANG Mengjia¹^,², FENG Xiangqian¹^,³, QIN Jinhua¹^,³, WANG Aidong¹, MA Hengyu¹, CHU Guang¹, LIU Yuanhui¹, XU Chunmei¹, ZHANG Xiufu¹, WANG Danying¹, ZHENG Xi⁴^,^*(), CHEN Song¹^,^*()

¹China National Rice Research Institute, Hangzhou 311401, China
²Binzhou Zhongyu Agricultural Science Research Institute, Binzhou 256600, China
³College of Agriculture, Yangtze University, Jingzhou 434025, China
⁴Life Sciences Division, Center of Electron Microscopy, Zhejiang University, Hangzhou 310058, China

Received:2023-09-25 Revised:2024-01-16 Online:2024-09-10 Published:2024-09-10
Contact: *email: xzheng@zju.edu.cn; chensong02@caas.cn

摘要/Abstract

摘要：

【目的】探讨不同水旱轮作模式与水稻季施氮水平在稻田土壤固碳(有机碳储量及其官能团特征)中的作用。【方法】依托中国水稻研究所水-旱轮作长期定位试验(2003年至今)，研究4种轮作模式[水稻-冬闲(RF)、水稻-紫云英(RC)、水稻-小麦(RW)和水稻-稻草覆盖种植马铃薯(RP)]与2个水稻季氮肥处理[不施氮(N0，0 kg/hm²) 和正常施氮(N1，135 kg/hm²)]对稻田土壤有机碳储量(0−50 cm)和有机碳官能团特征(0−20 cm)的影响。【结果】1) 在土壤浅耕层(0−20 cm)内，轮作能够在一定程度上提高有机碳含量，表现为RP>RC>RW或RF；但对于全耕层(0−50、0−40和0−30 cm)土壤有机碳储量而言，冬作(RP，RC和RW)与冬闲(RF)无显著差异，而施氮更有利于土壤固碳。2)水稻季不施氮处理下：相较于RF，冬作模式显著增加包括烷基碳和芳香碳在内的难降解组分，而抑制以烷氧碳为主的易降解组分，明显提高了土壤腐殖化指数、芳香性和疏水性，助力浅层土壤(0－20 cm)总有机碳储备的增加。其中，腐殖化指数和疏水性与还田秸秆C/N比值关系密切，这在浅层土壤固碳上具有重要意义。【结论】相较于轮作，适度施氮对全层土壤有机碳储备更有意义；而周年水旱轮作中全量秸秆还田对土壤有机质积累的效应仅停留在浅耕层，对全土层土壤有机碳储备的作用则被高估。秸秆碳在稻田的去向还有待进一步探索。

关键词: 稻田, 水旱轮作, 土壤有机碳储量, 官能团组分, ¹³C核磁共振

Abstract:

【Objective】The aim of this study is to clarify the role of paddy-upland crop rotation patterns and nitrogen fertilizer levels in carbon sequestration (organic carbon storage and chemical functional group characteristics) in paddy soils.【Method】Relying on the long-term paddy-upland crop rotation experiments in the China National Rice Research Institute (2003 to present), we investigated the effects of four paddy-upland crop rotation patterns: rice-fallow (RF), rice-green manure (Chinese milk vetch; RC), rice-wheat (RW), and rice-potato with rice straw mulch (RP) at two nitrogen levels during the rice growing season: no nitrogen application (N0, 0 kg/hm²) and normal nitrogen application (N1, 135 kg/hm²) on soil organic carbon stocks (0－50 cm) and the characteristics of soil organic carbon functional groups (0－20 cm) in paddy fields. 【Result】1) Within the shallow tillage layer (0－20 cm) of the soil, crop rotation was able to enhance soil organic carbon content to some extent, with the order of effectiveness being RP > RC > RW or RF. However, for soil organic carbon stocks in the full tillage layer (0－50, 0－40, and 0－30 cm), there were no significant differences between the winter crop rotations (RP, RC, and RW) and RF. In contrast, nitrogen application was found to be more favorable for soil carbon sequestration. 2) In the N0 treatment during the rice growing season after winter cropping, compared to RF, winter crop rotations (RP, RC, and RW) significantly increased the stable forms of carbon, including Alkyl C and Aromatic C, while suppressing the labile form of carbon dominated by O-Alkyl C. This treatment also significantly increased the soil humification index (HI), aromaticity (fa), and hydrophobicity, contributing to an increase in total soil organic carbon reserves in the surface layer (0－20 cm). It is noteworthy that the HI and hydrophobicity are closely associated with the C/N ratio of returned straw, which is important for promoting soil carbon sequestration in shallow soils. 【Conclusion】In comparison to crop rotation, moderate nitrogen application has shown to be more favorable for soil organic carbon reserves throughout the soil profile. On the other hand, the impact of full straw return on soil organic matter accumulation in the annual upland-paddy rotation was primarily observed in the shallow tillage layer, indicating that the effect of straw return on soil organic carbon accumulation might be overestimated when considering the entire soil profile. Therefore, further exploration is needed to better understand the fate and destination of straw carbon in paddy fields.

Key words: paddy field, upland-paddy rotation, soil organic carbon storage, functional groups, ¹³C-NMR

周子榆, 王孟佳, 冯向前, 覃金华, 王爱冬, 马横宇, 褚光, 刘元辉, 徐春梅, 章秀福, 王丹英, 郑希, 陈松. 轮作模式和氮肥处理对稻田土壤有机碳储量及其结构的影响[J]. 中国水稻科学, 2024, 38(5): 577-590.

ZHOU Ziyu, WANG Mengjia, FENG Xiangqian, QIN Jinhua, WANG Aidong, MA Hengyu, CHU Guang, LIU Yuanhui, XU Chunmei, ZHANG Xiufu, WANG Danying, ZHENG Xi, CHEN Song. Effects of Crop Rotation Patterns and Nitrogen Fertilizer Levels on Storage and Structure of Soil Organic Carbon in Paddy Fields[J]. Chinese Journal OF Rice Science, 2024, 38(5): 577-590.

图/表 12

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轮作 Rotation	施氮量 Nitrogen	TN (g/kg)	TP (g/kg)	AN (mg/kg)	SOM (g/kg)	pH
RF	N0	1.99±0.16 d	0.78±0.04 c	164.98±9.67 de	34.71±1.51 d	6.29±0.04 a
RF	N1	2.11±0.02 bcd	0.77±0.03 c	181.77±6.20 abcd	39.52±0.74 abc	5.95±0.01 cd
RC	N0	2.19±0.07 abcd	0.83±0.05 c	170.51±7.06 bcde	38.56±0.36 abcd	6.14±0.04 ab
RC	N1	2.23±0.06 abc	0.84±0.05 c	183.18±3.84 abc	37.88±0.95 bcd	5.94±0.05 cd
RP	N0	2.30±0.09 ab	1.03±0.05 ab	185.91±3.41 ab	40.66±2.54 ab	6.13±0.07 b
RP	N1	2.41±0.03 a	1.16±0.10 a	192.25±5.19 a	42.29±0.83 a	5.83±0.08 d
RW	N0	2.30±0.07 cd	1.03±0.07 bc	163.42±7.09 e	36.27±1.43 cd	6.05±0.07 bc
RW	N1	2.12±0.05 bcd	0.80±0.04 c	166.37±4.30 cde	37.41±1.67 bcd	5.86±0.03 d

轮作 Rotation	施氮量 Nitrogen	TN (g/kg)	TP (g/kg)	AN (mg/kg)	SOM (g/kg)	pH
RF	N0	1.99±0.16 d	0.78±0.04 c	164.98±9.67 de	34.71±1.51 d	6.29±0.04 a
RF	N1	2.11±0.02 bcd	0.77±0.03 c	181.77±6.20 abcd	39.52±0.74 abc	5.95±0.01 cd
RC	N0	2.19±0.07 abcd	0.83±0.05 c	170.51±7.06 bcde	38.56±0.36 abcd	6.14±0.04 ab
RC	N1	2.23±0.06 abc	0.84±0.05 c	183.18±3.84 abc	37.88±0.95 bcd	5.94±0.05 cd
RP	N0	2.30±0.09 ab	1.03±0.05 ab	185.91±3.41 ab	40.66±2.54 ab	6.13±0.07 b
RP	N1	2.41±0.03 a	1.16±0.10 a	192.25±5.19 a	42.29±0.83 a	5.83±0.08 d
RW	N0	2.30±0.07 cd	1.03±0.07 bc	163.42±7.09 e	36.27±1.43 cd	6.05±0.07 bc
RW	N1	2.12±0.05 bcd	0.80±0.04 c	166.37±4.30 cde	37.41±1.67 bcd	5.86±0.03 d

轮作 Rotation	施氮量 Nitrogen	DW(g/m²)	N(g/m²)	C(g/m²)	C/N
RC	N0	316.08±37.94	11.14±0.86	105.02±11.46	9.38±0.32
RC	N1	276.86±26.33	9.53±0.87	92.40±6.13	9.76±0.46
RP	N0	1789.56±152.52	16.02±2.35	531.38±89.49	33.02±2.62
RP	N1	1273.92±260.37	12.29±1.34	332.85±110.93	25.93±6.10
RW	N0	610.41±60.67	4.24±0.66	198.19±19.32	49.44±10.55
RW	N1	722.56±81.98	4.78±1.05	244.03±30.22	53.53±5.98

轮作 Rotation	施氮量 Nitrogen	DW(g/m²)	N(g/m²)	C(g/m²)	C/N
RC	N0	316.08±37.94	11.14±0.86	105.02±11.46	9.38±0.32
RC	N1	276.86±26.33	9.53±0.87	92.40±6.13	9.76±0.46
RP	N0	1789.56±152.52	16.02±2.35	531.38±89.49	33.02±2.62
RP	N1	1273.92±260.37	12.29±1.34	332.85±110.93	25.93±6.10
RW	N0	610.41±60.67	4.24±0.66	198.19±19.32	49.44±10.55
RW	N1	722.56±81.98	4.78±1.05	244.03±30.22	53.53±5.98

化学位移 Chemical shift(ppm)	有机物官能团 Functional groups of organic matter
0~45	烷基碳Alkyl C
45~60	烷氧碳O-Alkly C	含氮烷基+甲氧基碳N-Alkyl+Methoxyl C
60~94		炔基碳Alkyne C
94~110		双氧烷基碳di-O-Alkyl C
110~142	芳香碳Aromatic C	芳香基+苯烷基碳Aromatic+Aryl C
142~160		酚芳基碳Phenolic C
160~212	羰基碳Carboxyl C

轮作模式和氮肥处理对稻田土壤有机碳储量及其结构的影响

Effects of Crop Rotation Patterns and Nitrogen Fertilizer Levels on Storage and Structure of Soil Organic Carbon in Paddy Fields

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Metrics

ANOVA	SOCC(g/kg)	BD (g/cm³)	SOCD (kg/m²)
土层深度Depth(D)	1142.85^***	271.28^***	394.19^***
氮肥Nitrogen(N)	44.48^***	3.26	21.90^***
轮作Rotation(R)	5.61^**	5.02^**	0.64
D×N	5.07^**	2.22	4.30^**
D×R	9.78^***	2.32^*	6.46^***
N×R	0.06	1.27	0.73
D×N×R	9.11^***	0.78	7.94^***

土壤有机碳储量 SOCS	土壤有机碳含量SOCC					土壤容重BD
土壤有机碳储量 SOCS	0−10 cm	10−20 cm	20−30 cm	30−40 cm	40−50 cm	0−10 cm	10−20 cm	20−30 cm	30−40 cm	40−50 cm
0~50 cm	0.04	0.10	0.30	0.53**	0.51*	0.25	0.14	0.10	0.08	0.50*
0~40 cm	0.10	0.10	0.43*	0.38		0.23	0.12	0.12	0.10
0~30 cm	0.35	0.47*	0.63**			0.02	0.11	0.32
0~20 cm	0.59**	0.64**				0.13	−0.07

水稻生育时期 Rice growth stage	施氮量 Nitrogen level	轮作 Rotation	烷基碳 Alkyl C(%)	烷氧碳 O-Alkly C(%)
水稻生育时期 Rice growth stage	施氮量 Nitrogen level	轮作 Rotation	烷基碳 Alkyl C(%)	含氮烷基+甲氧基碳N-Alkyl+Methoxyl C	炔基碳 Alkyne C	双氧烷基碳di-O-Alkyl C	合计 Total
水稻移栽前 Before transplanting	N0	RF	18.60±0.41 cd	12.22±0.57 a	36.51±1.05 a	5.15±0.34 bc	53.87±1.51 a
		RC	20.39±0.39 bc	12.57±0.78 a	34.32±0.83 a	6.86±0.60 a	53.75±0.52 a
		RP	26.95±0.10 a	8.71±0.01 b	30.11±1.09 b	5.60±0.22 bc	44.42±1.12 b
		RW	26.28±0.82 a	11.98±0.50 a	25.70±0.32 c	5.57±0.60 bc	43.25±0.88 b
	N1	RF	21.98±0.45 b	5.56±0.83 c	24.69±0.72 cd	4.67±0.44 c	34.92±1.04 de
		RC	17.52±0.71 d	5.81±0.50 c	25.98±1.14 c	4.71±0.37 c	36.50±1.80 cd
		RP	18.63±1.22 cd	13.13±0.39 a	19.23±0.70 e	6.35±0.11 ab	38.71±0.41 c
		RW	18.88±0.30 cd	4.56±0.28 c	22.81±0.80 d	4.43±0.44 c	31.80±0.69 e
水稻收获后 After harvest	N0	RF	17.25±0.58 bc	16.61±0.58 ab	19.92±0.33 cd	5.80±0.28 cd	42.33±0.24 bc
		RC	19.43±0.60 ab	15.79±1.39 bc	20.59±0.76 bcd	4.68±0.25 de	41.07±0.95 bc
		RP	14.60±0.61 d	18.60±0.61 a	20.59±0.50 bcd	7.35±0.72 b	46.54±0.53 a
		RW	11.67±0.57 e	14.01±0.68 cd	21.77±0.52 bc	6.64±0.57 bc	42.42±0.78 bc
	N1	RF	19.78±0.54 a	12.75±0.50 d	19.62±0.35 d	5.73±0.28 cde	38.10±1.01 d
		RC	21.51±0.69 a	15.72±0.48 bc	20.08±0.26 cd	4.41±0.50 e	40.21±0.31 cd
		RP	14.27±1.44 d	9.13±0.22 e	24.66±1.01 a	8.71±0.53 a	42.50±0.67 b
		RW	15.75±0.16 cd	11.70±1.51 de	22.35±0.91 b	4.55±0.11 de	38.60±1.03 d
水稻生育时期 Rice growth stage	施氮量 Nitrogen level	轮作 Rotation	芳香碳 Aromatic C(%)			羰基碳 Carboxyl C(%)
水稻生育时期 Rice growth stage	施氮量 Nitrogen level	轮作 Rotation	芳香基+苯烷基碳Aromatic+Aryl C	酚芳基碳 Phenolic C	合计 Total	羰基碳 Carboxyl C(%)
水稻移栽前 Before transplanting	N0	RF	10.40±0.53 ab	2.51±0.22 d	12.91±0.70 cd	14.62±1.23 d
		RC	9.61±0.46 b	2.28±0.20 d	11.90±0.64 d	13.97±0.45 d
		RP	11.50±1.25 ab	3.74±0.10 ab	15.24±1.33 abc	13.40±0.15 d
		RW	12.64±1.81 a	3.74±0.21 ab	16.38±1.85 ab	14.09±0.59 d
	N1	RF	8.85±0.42 b	3.45±0.29 bc	12.29±0.42 d	30.81±1.04 b
		RC	9.47±0.48 b	4.40±0.31 a	13.87±0.24 bcd	32.11±1.26 b
		RP	12.87±0.74 a	4.28±0.35 a	17.15±0.81 a	25.52±0.47 c
		RW	11.48±0.57 ab	2.97±0.03 cd	14.45±0.60 abcd	34.87±1.06 a
水稻收获后 After harvest	N0	RF	13.68±0.23 ab	3.52±1.01 ab	17.19±1.14 ab	23.22±1.13 d
		RC	13.90±0.79 ab	2.77±0.31 b	16.68±0.53 abc	22.83±0.96 d
		RP	10.73±0.12 d	4.28±0.16 a	15.00±0.27 c	23.86±0.30 cd
		RW	12.99±0.46 bc	4.12±0.11 ab	17.12±0.38 ab	28.80±0.60 a
	N1	RF	12.18±0.10 c	3.74±0.18 ab	15.92±0.16 bc	26.20±0.64 b
		RC	9.90±0.37 d	2.97±0.39 ab	12.87±0.70 d	25.41±0.36 bc
		RP	15.09±0.52 a	3.26±0.59 ab	18.36±0.26 a	24.87±0.87 bcd
		RW	12.66±0.70 bc	3.64±0.41 ab	16.30±1.00 bc	29.37±0.35 a

水稻生育时期 Rice growth stage	施氮量 Nitrogen level	轮作 Rotation	腐殖化指数 HI	芳香性 fa	疏水性 Hydrophobicity
水稻移栽前 Before transplanting	N0	RF	0.35±0.00 ef	0.15±0.01 gh	0.46±0.01 efgh
		RC	0.38±0.01 ef	0.14±0.01 h	0.48±0.02 defg
		RP	0.61±0.01 a	0.18±0.02 fg	0.73±0.04 a
		RW	0.61±0.02 a	0.19±0.02 ef	0.74±0.04 a
	N1	RF	0.63±0.03 a	0.18±0.00 fg	0.52±0.02 bcde
		RC	0.48±0.04 c	0.20±0.00 cde	0.46±0.02 fgh
		RP	0.48±0.03 c	0.23±0.01 abc	0.56±0.01 bc
		RW	0.59±0.01 ab	0.22±0.01 abcd	0.50±0.01 cdef
水稻收获后 After harvest	N0	RF	0.41±0.01 de	0.23±0.01 abc	0.53±0.03 bcd
		RC	0.47±0.02 cd	0.22±0.00 bcde	0.56±0.01 b
		RP	0.31±0.02 fg	0.20±0.00 def	0.42±0.01 gh
		RW	0.28±0.01 g	0.24±0.01 ab	0.40±0.01 h
	N1	RF	0.52±0.03 c	0.22±0.00 abcd	0.56±0.01 bc
		RC	0.53±0.02 bc	0.17±0.01 fg	0.52±0.01 bcde
		RP	0.34±0.04 fg	0.25±0.00 a	0.48±0.03 def
		RW	0.41±0.01 de	0.23±0.01 abc	0.47±0.02 defg

土壤有机碳质量特征指数 Soil organic carbon quality characteristics index	DW	N	C	C/N
腐殖化指数HI	0.41	−0.24	0.41	0.72**
芳香性fa	0.19	−0.25	0.13	0.43
疏水性Hydrophobicity	0.54*	0.050	0.50*	0.50*

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