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10 January 2006, Volume 20 Issue 1 Previous Issue    Next Issue
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研究报告
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SSR Marker-based Genetic Diversity Fingerprinting of Hybrid Rice in Sichuan,China XIAO Xiao-yu ,WANG Yu-ping ,ZHANG Jian-yong ,LI Shi-gui ,RONG Ting-zhao 2006, 20(1): 1-7 .  Abstract ( )   PDF (597KB) ( )   A total of 208 SSR primers were used to detect 42 major parents of hybrid rice that were widely popularized in Sichuan Province. Polymorphism were detected with 123 pairs of the primers accounting for 59.13% of the total. Fingerprinting maps of these 42 widely used parents were constructed. The results showed significantly higher genetic diversity on chromosomes 9 and 10 and considerably lower diversity on chromosome 12. The genetic diversity of these materials was limited, but it was remote correspondingly between the restorer lines and sterile lines. Generally, important parents of hybrid rice can be identified by their particular primers. Out of the primers, 12 and 10 respectively were selected and they were polymorphic between two parents, which were adapted to examine the purity of hybrid rice seed. The seed purity of D you 527 was identified by the particular SSR primers RM337, RM244 and RM346, and it was agreeable with the result of the field test. Two combinations, D you 527 and D you 68, which rooted in the same sterile line, were distinguished by the particular SSR primer for D you 527. It showed that SSR technique has broad applicability in variety protection, authenticity and purity identification.

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Establishment and Application of a Real-Time Fluorescence Quantitative PCR for Detecting Transcripts of Low Abundance Gene, OsAMT1;3,in Rice SUN Shu-bin ,LI Bao-zhen ,HU Jiang ,XU Guo-hua 2006, 20(1): 8-12 .  Abstract ( )   PDF (171KB) ( )   One of the ammonium transporter genes in rice,OsAMT1;3,normally expressed in low abundance,which is difficult to quantify its expression by using traditional methods,such as Northern blot,PCR technique. A technique for real-time quantification of OsAMT1;3 relative to a housekeeping gene for encoding an actin in rice using real-time SYBR Green quantitative PCR(RQ-PCR) with specific primers was established,which showed:the amplification curve had flat baseline,distinct exponential area,large and stable slope; The coefficient of variation of the technique was 0.47%; There was a linear relationship between threshold cycle value at which sample crosses threshold and the logarithmic value of template concentration; The expression of OsAMT1;3 was enhanced 4-fold by nitrogen starvation in comparison to supply of ammonium as sole source of nitrogen.

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Overexpression of Two Different Transcription Factors, OPBP1 and OsiWRKY, Enhances Resistance Against Pathogen Attack and Salt Stress in Rice LI Nan-yi ,GUO Ze-jian 2006, 20(1): 13-18 .  Abstract ( )   PDF (429KB) ( )   Two transcription factors, OPBP1 and OsiWRKY, involved in the regulation of signal transduction pathways under salt stress and disease resistance, respectively, were co-transformed into japonica rice Xiushui 11 by particle bombardment. PCR amplification, Southern blot and Northern blot analyses indicated that OPBP1 and OsiWRKY had been integrated into rice genome and overexpressed in rice. Compared with non-transgenic plants, transgenic lines exhibited high resistance to Magnaporthe grisea and Xanthomonas oryzae pv. oryzae, and showed improved resistance to salt stress. Under the same salt stress conditions, transgenic plants grew faster, had significant higher biomass and chlorophyll content compared to non-transgenic plants. Furthermore, it was found that higher expression level of the transgene had higher salt tolerance and disease resistance. These results suggested the overexpression of OPBP1 and OsiWRKY enhanced resistance to salt stress and pathogens in rice.

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Relationship Between Coleoptile Length and Drought Resistance Index of Rice and Their QTLs HU Song-ping ,YANG Hua ,ZOU Gui-hua ,LIU Hong-yan ,LIU Guo-lan ,MEI Han-wei ,CAI Run ,LI Ming-shou ,LUO Li-jun 2006, 20(1): 19-24 .  Abstract ( )   PDF (159KB) ( )   A set of recombinant inbred lines (RILs) population including 195 lines derived from a cross of Zhenshan 97B (lowland rice variety) and IRAT109 (upland rice variety) was used for correlation analysis and QTL identification for coleoptile length (CL) and drought resistance index (DRI). The correlation coefficient between CL and DRI under water stress was 0.2206 ** . A total of 13 and 5 QTLs for CL and DRI under different conditions were identified, respectively. The phenotypic variance explained by each QTL ranged from 2.28% to 22.65%. qCL9 possessed the same location (RM160-RM215) with qDRI9. Seventeen epistasis loci for CL were detected with 58.35% of total contribution; three pairs of epistasis loci for DRI were detected with 11.93% of total contribution. Comparing the QTLs related to drought resistance in other studies, QTLs of CL and DRI were located in the same or adjacent marker interval with the QTLs related to root traits, for instance, dry weight, root depth, root number and root length.

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Expression of Plant Height and Sensitivity to GA3 of Dominant Semi-Dwarfing Gene in Rice CHENG Can ,WU Yue-jin ,LIU Bin-mei ,TONG Ji-ping ,WU Jing-de ,ZHANG Ying ,WU Jin-hua ,YUAN Qin 2006, 20(1): 25-30 .  Abstract ( )   PDF (222KB) ( )   Six tall and dwarf near isogenic lines derived from a dominant semi-dwarfing mutant were studied in plant height traits and sensitivity to GA 3 . The internode elongation was inhibited by the dominant semi-dwarfing gene. The lengths from the basal internode to the uppermost internode and the panicle length in dwarf near isogenic lines were 97.2%,53.3%,65.1%,61.9% and 94.7% of those in the tall ones, respectively. Compared with wild type Y98148, dwarf mutant Y98149 was much more sensitive to GA 3 at both the seedling and adult stages, and showed lower endogenic GA 3 content in plant, with about 78% of the wild one.

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Analysis on Genetic Background and Photosynthetic Characteristics of the Improved Shuhui 881 with Maize C4-type pepc Gene HE Li-bin ,XIANG Xun-chao ,LI Ji-hang ,ZHONG Li ,ZHANG Kai-zheng ,LI Ping 2006, 20(1): 31-35 .  Abstract ( )   PDF (215KB) ( )   The genetic background of ten improved plants of Shuhui 881 with maize C 4 -type pepc gene (pepc Shuhui 881) were analyzed with 530 pairs of SSR primers, only 1-4 differential SSR loci were found compared with Shuhui 881, and the similarities of genetic background were 95.15%-99.03%. By using pepc Shuhui 881 with a similarity at 99.03% as materials, the photosynthetic characteristics were determined at the six different developmental stages. The PEPCase activities in the improved Shuhui 881 were higher than those in Shuhui 881. The highest PEPCase activity was 1264.2 μmol/(mg·h) at the elongation stage and the largest increased value of PEPCase activity in the pepc Shuhui 881 was 23.3-fold than that in Shuhui 881 at the initial-tillering stage. The net photosynthesis rates of the pepc Shuhui 881 increased at the initial-tillering, full-tillering, elongation and initial-heading stages, and peaked at 22.3% at the elongation stage. The results showed that the genetic background of the pepc Shuhui 881 were almost the same as Shuhui 881 by molecular marker-assisted selection(MAS) and the high expression level of maize C 4 -type pepc gene was stably inherited in indica genetic background. Therefore, the system for selecting high photosynthetic efficiency rice had been established, which included selecting rice by MAS with the characteristic primers of C 4 -type pepc gene marker, determining PEPCase activity and net photosynthesis rate and investigating phenotype performance in field. Meanwhile, it would be better to determine photosynthetic characteristics at the elongation stage.

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Developmental Genetics of Valine Content at Different Grain Filling Stages in indica-japonica Hybrid Rice ZHANG Xiao-ming ,SHI Chun-hai ,YE Sheng-hai ,WU Jian-guo ,BAO Gen-liang 2006, 20(1): 36-42 .  Abstract ( )   PDF (154KB) ( )   Two developmental genetic models for quantitative traits of seeds in cereal crops, i.e. unconditional model referred to the analysis of cumulative measurements along the developmental stages, and conditional model referred to the gain analysis throughout the developmental stages, were used to analyse the developmental genetics of valine content at different grain filling stages in the indica-japonica hybrid rice. The valine content was governed by diploid maternal plant genes, triploid endosperm genes, cytoplasmic genes and their environmental interaction effects. Those genes were interval expressions among grain filling stages, and the most active stage when genes expressed was 8-14 days after flowering. Heritability analysis expressed that maternal heritability, cytoplasmic heritability and their environmental interactions were important at all the five filling stages. Single plant selection during early breeding generations would be effective to enhance the content of valine in rice breeding. Prediction of genetic effects in rice parents showed that Zhe 38A, Zhenong 8010A, Jin 23A, and Zhong 9308 could be used for improving the valine content in indica-japonica hybrids. The results also showed that Zhe 38A was more stable in different environmental conditions, it is the best one of the 12 parents tested for improving the valine content in rice breeding.

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Caryopsis Development and Main Grain Quality Characteristics in Different indica Rice Varieties XIONG Fei ,WANG Zhong ,CHEN Gang ,WANG Jue 2006, 20(1): 43-46 .  Abstract ( )   PDF (861KB) ( )   A comparison of the caryopsis development, rice quality characteristics and the size, pattern, structure of the endosperm amyloplasts (starch granules) in the two indica rice varieties was made.The quality traits in Yangdao 6 were better than those in Xiangzaoxian 33. In the primary period after flowering, the dry matter in Yangdao 6 was accumulated more slowly than that in Xiangzaoxian 33 but faster in the later period, and the starch was filled strongly in the later period after flowering.There were two kinds of amyloplasts: single and compound amyloplasts, 4.7 μm and 9.7μm in diameter on average with the range of 2.4-8.0 μm and 5.7-19.5 μm, respectively. In the case of the endosperm of Xiangzaoxian 33, most of the single amyloplasts were elliptic or round with loose arrangement and great difference in size, and the coefficient of variation was high;While in the endosperm of Yangdao 6, most of the amyloplasts were single, well developed, polyhedral, crystalline and compact-arranged, and the coefficient of variation was low. The amyloplasts in dorsal part of endosperm were developed better than those in abdomen and center. The chalkiness in the endosperm resulted from badly-developed and loose-arranged starch, which was closely relevant to the transport of filling materials.

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Comparative Analysis and Investigation on Plants Growing in in situ Environments of Three Wild Rice Species in Yunnan Province WANG Lin ,DAI Lu-yuan ,WU Li-hua ,YANG Qing-wen ,XU Fu-rong ,YU Teng-qiong ,TANG Cui-feng 2006, 20(1): 47-52 .  Abstract ( )   PDF (128KB) ( )   Field survey was carried out on the plants growing in 40 in situ sites of three wild rice species in Yunnan Province, China. A total of 159 plant species, distributed in 51 families were discovered and documented. The plants were classified into three ranks, i.e. plants at arbor, shrub and herb levels.There were 11 species fell into 6 families and 1 sub-family at arbor level, 60 ones into 29 families at shrub level and 88 ones into 20 families at herb level. Ninety-five percent of plants growing together with Oryza rufipogon Griff. belonged to herb level while the plants growing together with O. meyeriana Baill. included herb and arbor levels. There were 20 plant species fell into 5 families and none of them was discovered in any sites of the other two wild rices growing in the site of O. rufipogon. There were 25 plant species fell into 18 families discovered in the sites of O. officinalis Wall. and 15 species were specific. There were 125 plant species fell into 45 families discovered in the sites of O. meyeriana and 117 species were specific. Significance of investigation on plant species growing together with the three wild rices in situ and influence of exotic plants on the wild rices in situ was discussed.

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Effects of Free-Air Carbon Dioxide Enrichment(FACE) on Some Traits and C/N Ratio of Rice Root at the Heading Stage CHEN Gai-ping ,ZHU Jian-guo ,PANG Jing ,CHENG Lei ,XIE Zu-bin ,ZENG Qing 2006, 20(1): 53-57 .  Abstract ( )   PDF (133KB) ( )   Rice root growth was examined by using hydroponics in a FACE(free-air carbon dioxide enrichment)study. Root biomass, root volume, ratio of root/shoot, number of adventitious roots and root diameter were all increased significantly under FACE conditions. CO 2 enrichment decreased the N content in the rice root, but did not change the C content, leading to an obvious increase in the ratio of C/N. Elevated CO 2 decreased the rice root activity remarkably. Decrement in nutrient uptake efficiency may be the mechanism of decreased N content in root.

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Relationship Between Activity of Key Starch Synthetic Enzymes During Grain Filling and Quality of Eating and Cooking in Rice SHEN Peng ,JIN Zheng-xun ,LUO Qiu-xiang ,JIN Xue-yong ,SUN Yan-li 2006, 20(1): 58-64 .  Abstract ( )   PDF (151KB) ( )   Four japonica rice varieties with significant differences in quality of eating and cooking were investigated to reveal the relationship between the key starch synthetic activities during grain filling and quality of eating and cooking of rice. The varieties presented the differences in amylose and amylopectin contents at the different grain filling stages. The differences were attributed to the various accumulation speed of starch during grain filling. The time when the enzyme activities reached a maximum for ADPglucose pyrophosphorylase (AGPP) and soluble starch synthesis (SSS) were not different in different quality types rice, but was different for starch branching enzyme (SBE), showed that the low quality varieties were earlier than that of the high quality ones, and high quality varieties still kept high enzyme activities at the late stages of grain filling. The correlation and correlative degree between AGPP, SSS, SBE and amylose content, amylopectin content, taste meter value, properties of amylogram varied with the different stage of grain filling. The correlation between soluble starch synthesis activity and taste meter value was not significant during the whole stage of grain filling, but at the early and late stage of grain filling, the activities of AGPP and SBE had significant or highly significant correlation with taste meter value. It is helpful for improving quality of eating and cooking of japonica rice by using the materials with low enzyme activity at the early stage or high enzyme activity at the late stage of grain filling as parents.

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Analysis on Source-Sink and Grain-Filling Characteristics of Two-Line Hybrid RiceYangliangyou 6 ZHAO Bu-hong ,WANG Peng ,ZHANG Hong-xi ,ZHU Qing-sen ,YANG Jian-chang 2006, 20(1): 65-72 .  Abstract ( )   PDF (799KB) ( )   With two-line hybrid rice Yangliangyou 6 and Liangyoupeijiu and three-line hybrid rice Shanyou 63 as materials, the relationships of source, sink and flow with grain filling were studied. The seed-setting rate, grain filling degree and grain yield of Yangliangyou 6 and Shanyou 63 were significantly higher than those of Liangyoupeijiu. The export percentage and transformation percentage of the matter in culms and sheaths of Yangliangyou 6 and Shanyou 63 were significantly higher than those of Liangyoupeijiu. Activities of sucrose synthase, adenosine diphosphoglucose pyrophosphorylase, starch synthase and starch branching enzyme in grains were higher in Yangliangyou 6 and Shanyou 63 than in Liangyoupeijiu, and were very significantly correlated with maximum grain filling rate, mean grain filling rate, grain filling degree and grain weight. The spikelet number, grain yield and total sink load per area of vascular bundle and phloem of Yangliangyou 6 and Shanyou 63 were significantly smaller than those of Liangyoupeijiu, and the greater the load, the lower the seed-setting rate and the poorer the grain filling. The transportation rate per area phloem of Yangliangyou 6 was greater than that of Liangyoupeijiu or Shanyou 63. The results suggested that Yangliangyou 6 possesses strong source, great sink activity and efficient flow, which lay a physiological basis for its high seed-setting rate and good grain filling.

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Effect of Deep Application of Fertilizer in the Whole Growing Period on Yield Formation and Quality of Rice LI Dian-ping ,CAO Hai-feng ,ZHANG Jun-bao ,JIN Xue-yong 2006, 20(1): 73-78 .  Abstract ( )   PDF (144KB) ( )   By using a super-high yielding rice variety Dongnong 423 as material, the effect of four kinds of fertilizer application methods was studied based upon the investigation on some characters of growth and development in rice, including tiller number, leaf chlorophyll content, leaf area index, amount of dry matter accumulation in plant, and yield and its components, as well as protein content, amylose content and taste meter value of rice. The four fertilization methods were deep application in the whole growth period (DAF), incorporating into all soil layers (IAF), surface application(SAF) and no fertilization(NF). The DAF treatment could prolong the duration of effective tillering, increase leaf chlorophyll content, leaf area index and amount of dry matter accumulation in plant compared with the other three methods. There were significant differences(P=0.01) in grain yield and rice protein content (DAF>IAF>SAF>NF), and in amylose content and taste meter value of rice(P<0.01)(NF>SAF>IAF>DAF). Under DAF treatment, the grain yield, number of panicles, number of grains per panicle and rice protein content were increased, however, the seed setting rate,1000-grain weight, amylose content and taste meter value of rice were decreased compared with the other three treatments.

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Effect of Proportion of Nitrogen Fertilizer for Promoting Panicle Development on Nitrogen Uptake, Grain Amino Acid Contents and Grain Yield of Two-Line Hybrid Rice DAI Ping-an ,ZHENG Sheng-xian ,LI Xue-bin ,NIE Jun ,YI Guo-ying ,YUAN Di-ren ,HUANG Ke-yan 2006, 20(1): 79-83 .  Abstract ( )   PDF (262KB) ( )   The field and pot experiments were conducted to study the effect of the allocating ratio of N-fertilizer on nitrogen uptake, grain amino acid contents and grain yield of two-line hybrid rice. For two-line hybrid rice (early season),the grain yield at the treatment of N application of 180 kg/hm2, 30% N for panicle development(applied at the flag leaf appearing in the main culm)and 70% N for basal fertilizer was the highest, and both increased by 6.2% compared to the treatments of the same N application (180 kg/hm2 ) with 10% N for panicle development and N application of 120 kg/hm 2 with 30% N for panicle development. For late season, the N allocating ratio getting the highest yield at the N application of 210 kg/hm2 was 30% N for panicle development, too. The result of three different N application levels showed that, the order of N recovery and agronomic efficiency was 30%>20%>10%, N use efficiency was 30%<20%<10%, at the treatment of different N allocating ratios. The essential amino acid contents in brown rice under the N allocating ratio of 30% for panicle development and 70% for basal fertilizer was increased by 0.09―0.38 percent point compared with other treatments of different N allocating ratios. Among the essential amino acid contents increased, Leu and Ile contents were increased the most, followed by the Lys content. The yield of brown rice, the accumulated amount of amino acids and essential amino acids under this treatment was also increased by 5.4%-27.5%, 5.4%-30.4% and 15.1%-36.0% compared with other treatments, respectively.

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Community Composition of Gram-Positive Bacteria Associated with Rice and Their Antagonists Against the Pathogens of Sheath Blight and Bakanae Disease of Rice LI Bin ,XIE Guan-lin ,LU Yi-lin ,HAO Xiao-juan ,LUO Jin-yan ,LIU Bo ,LI Wen 2006, 20(1): 84-88 .  Abstract ( )   PDF (115KB) ( )   A survey on screening and identification of gram-positive bacteria was conducted during 1998-2004. Seven hundred and fifty-six rice samples were collected from Zhejiang, Jiangsu, Fujian and Yunnan Provinces, China. Over 1000 bacterial isolates were isolated and tested with colony morphology, pathogenicity, and some characteristics of bacteriology including Gram staining, fluorescent pigment on Kings medium B and microscopic observation for endospore. Together with 5 standard reference strains, 74 representative gram-positive bacterial isolates were confirmed by Biolog and gas chromatographic analysis of fatty acid methyl esters. Five bacterial species of Bacillus and other 3 genera were identified and the isolates from Bacillus sublitis and Bacillus megaterium exhibited the most effective inhibition against the pathogens of sheath blight and bakanae disease of rice. A few isolates from Bacillus pumilus and Bacillus megaterium showed weak virulent on rice together with some virulent isolates, risk should be considered when isolates from these species were screened for biocontrol agents.

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Seed Bank of Perennial Weeds in Paddy Fields WU Jing-lun ,ZHOU Heng-chang 2006, 20(1): 89-96 .  Abstract ( )   PDF (151KB) ( )   The investigation was conducted in the system of rice-wheat rotation in Yizheng, Jiangsu Province to determine the category, distribution, propagation and germination of the perennial weeds. There were five species of perennial weeds, i.e. Potamogeton distinctus,Sagittaria pygmaea, Eleocharis plantagineformi, Juncellus serotinus, and Scirpus planiculmis in the system. The seeds, tubers and corms of the weeds mainly distributed in the 0-15 cm depth paddy soil, and mostly executed vegetative propagation. The seeds germinated in the surface layer paddy soil, and the tubers and corms did in the 0-15 cm depth soil. The best conditions for the weeds germination were saturation soil moisture to thin layer water. The longevity of the seed, tuber and corm as well as the effect of non-tillage and paddy-upland rotation on weed seed bank were expatiated. The control measure to five perennial weeds species was given.

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Resistance and Mechanism of Four Selected Rice Varieties to Rice Stem Borers (Chilo suppressalis and Scirpophaga incertulas) in Shanghai Area, China LUO Ju ,ZHANG Xiao-xi ,ZHAI Bao-ping ,GUO Yu-ren 2006, 20(1): 97-101 .  Abstract ( )   PDF (259KB) ( )   Resistance and mechanism of four selected rice varieties to the rice stem borers (Chilo suppressalis Walker and Scirpophaga incertulas Walker) were analyzed in Shanghai rice cropping area. There were obvious differences of varietal resistance between Xiushui 04, the mainly-used variety in Shanghai area from the 1980s to 1990s, and the other three varieties, Hanyouxiangqing, Huayu 1 and 98110, which are released and popularized since the late stage of 1990s. The resistance of Xiushui 04 to the stem borers was significantly higher than those of the other three. At the elongation stage, the vascular bundle interval of Xiushui 04 was the smallest among the four varieties. Content of SiO 2 of Xiushui 04 was also the highest among the four. However, the other characteristics of the varieties, such as the proportions of other sixteen essential amino acids, amounts of N, P, K , as well as the structures of the rice stem tissue had no obvious or identical differences among the rice varieties.

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Mapping QTLs for Bacterial Blight Resistance in a DH Population from japonica/indica Cross of Rice (Oryza sativa) YANG Chang-deng ,ZENG Da-li ,MA Liang-yong ,JI Zhi-juan ,GUO Long-biao ,LI Xi-ming ,QIAN Qian 2006, 20(1): 102-104 .  Abstract ( )   PDF (113KB) ( )   A double haploid (DH) population derived from the cross between a japonica parent Chunjiang 06 and an indica parent TN1 was used to detect QTLs for resistance to rice bacterial blight(BB). Chunjiang 06 was moderately resistant to the strain of Xanthomonas oryzae pv. oryzae (Xoo), Zhe 173 and TN1 was susceptible to. Three QTLs (qBBR1,qBBR3-1, qBBR7) were detected for BB resistance on chromosomes 1, 3, 7, respectively. qBBR3-1 and qBBR7 showed additive effect, and qBBR1 negative. The result revealed that Chunjiang 06 did not carry any major resistance gene and two QTLs were detected on chromosomes 3 and 7 conferring the resistance to Xoo strain.

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Co-expression of CecropinB and Xa21 Genes Increased Resistance to Bacterial Blight inTransgenic Rice YU Yan-chun ,ZHANG Guang-heng ,GUO Long-biao ,ZENG Da-li ,ZHAO Yan ,HU Xing-ming ,HUANG Da-nian ,QIAN Qian 2006, 20(1): 105-108 .  Abstract ( )   PDF (220KB) ( )   To explore the effect of two disease resistance-encoding genes with different mechanisms on resistance against Xanthomonas oryzae pv. oryzae (Xoo), Agrobacterium-mediated transformation was used to acquire plants expressing CecropinB, Xa21, and both the genes, respectively, and which were verified by PCR analysis. The single transgene showed moderate resistance to all of the Xoo strains tested, and the resistance level of Xa21 was stronger than that of CecropinB. With the co-expression of CecropinB and Xa21, the resistance was enhanced, indicating that gene pyramiding with different resistance mechanisms is a potential strategy in developing rice plants with durable resistance.

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Primary Study on Correlation Between Pathotypes and DNA Fingerprintings Based on Avirulence Gene Sequences of Rice Blast Fungus Magnaporthe grisea WANG Jian-fei ,BAO Yong-mei ,LI Pei-fu ,ZHANG Hong-sheng 2006, 20(1): 109-112 .  Abstract ( )   PDF (194KB) ( )   According to the published sequences of avirulence genes of Magnaporthe grisea,four pairs of special primers were designed and used to amplify the types of DNA fingerprinting of 31 pathogenic isolates from various regions by PCR technique. Thirteen types of fingerprinting were obtained by assortment of the electrophoresis bands of PCR products, which showed no geographical properties except the strains from Guangdong, China. Meanwhile, the pathotypes of all isolates were detected through twice inoculation in seven Chinese differentials, seven near-isogenic lines with the genetic background of Lijiangxintuanheigu and twelve Japanese differential cultivars with a single R gene, respectively. The results showed that there were to some extent correlations between the DNA fingerprinting types of pathogen and their pathotypes based on Japanese differential cultivars.

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Genetic Analysis on the Resistance to the Brown Planthopper and Rice Gall Midge in Rice Variety RP1976-18-6-4-2 HUANG Feng-kuan ,WEI Su-mei ,LIANG Guang-wen ,HUANG Suo-sheng ,JIANG Xian-bin ,LUO Shan-yu ,LI Qing 2006, 20(1): 113-115 .  Abstract ( )   PDF (156KB) ( )   The resistance of rice variety RP1976-18-6-4-2 to the rice brown planthopper (BPH) and the rice gall midge (GM) was analyzed. RP1976-18-6-4-2 showed resistance to biotype Ⅱ, biotype Bangladesh of BPH and China type Ⅱ, China type Ⅳ of GM.Genetic analysis suggested that the resistance of RP1976-18-6-4-2 to biotype Ⅱand biotype Bangladesh of BPH were both controlled by a dominant gene and a recessive gene; and resistance to China type Ⅱ and China type Ⅳ of GM was controlled by a single dominant gene.

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Effect of Saline-Alkali Stress on Source-Sink and Yield of Rice ZHANG Rui-zhen ,SHAO Xi-wen ,TONG Shu-yuan ,WANG Heng-wu ,QI Chun-yan ,SUN Chang-zhan 2006, 20(1): 116-118 .  Abstract ( )   PDF (107KB) ( )   Two rice varieties with different tolerance to salinity and alkalinity were planted in four natural saline-alkali soils from different areas in the west of Jilin Province with pot experiment. Under saline-alkali stress, leaf area index was decreased, and the decreasing rate was larger in Jinongda 10 (susceptible variety) than that in 89-45(tolerant variety). Saline-alkali stress reduced effective tiller number and led to low grain yield of rice.