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10 January 2007, Volume 21 Issue 1 Previous Issue    Next Issue
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研究报告
综述与专论
研究简报
研究报告
研究简报


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Identification of Differentially Expressed Genes in Rice Plants Responded to Different Races of Blast Fungus Hu Hai-yan,Zhuang Jie-yun,Chai Rong-yue,Wu Jian-li,Fan Ye-yang,Zheng Kang-le 2007, 21(1): 1-6 .  Abstract ( )   PDF (273KB) ( )   RNAs isolated from plants of a rice cultivar inoculated with two different races of the blast fungus were used to construct two cDNA libraries enriched for transcripts differentially expressed in incompatible/compatible interactions through suppression subtractive hybridization (SSH). After differential screening and RT-PCR based confirmation, twenty-five unique cDNA clones showing differentially expressions in incompatible/compatible interactions were sequenced. These cDNA clones were with known functions according to the putative functions of their homologous genes in the database. They might be involved in pathogen response, transcription, protein synthesis and protein fate etc. Expression profilings of the 25 cDNA clones at the early stages after inoculation were also revealed by RT-PCR. All of their expression levels were identical at 0 h of inoculation, followed by inducing or suppressing in the two interactions, indicating that the expression changes of these genes were solely due to different races of the pathogen and the reliability of the selected genes in the present experiment. It is also postulated that in rice-Magnaporthe grisea interactions, the time points and levels of expressions of some defense-related genes might determine the race-specific resistances of the host.

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Microsatellite Marker-Based Analysis of Rice Varieties in National Regional Yield Trial of Southern China Cheng Ben-yi,Shi Yong-feng,Shen Wei-feng,Zhuang Jie-yun,Yang Shi-hua 2007, 21(1): 7-12 .  Abstract ( )   PDF (546KB) ( )   A total of 199 rice varieties in the national regional yield trial of southern China in 2005 were assayed with a preferred set of 12 microsatellite markers(SSR). A DNA fingerprint database containing genotypic data of the 199 rice varieties at the 12 marker loci was constructed. It was found that the dominant alleles in conventional rice varieties were the same as in hybrid rice at most of the marker loci, but they differed greatly from indica to japonica rice. Conventional rice was of high homozygosity and most hybrid rice was of high heterozygosity. The variation in number of heterozygous loci was evident for hybrid rice with a normal distribution. On comparison among hybrid rice combinations derived from the same cytoplasmic male sterile(CMS) line, general consistency of the maternal allele of major CMS lines was observed at the SSR loci. Moreover, the distinctness assay of rice variety in the national regional yield trial of southern China, and the expansion of DNA fingerprint data base and the number of SSR markers for a feasible test were discussed.

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Establishment and Application of Two-Dimensional Gel Electrophoresis Analysis of Proteins from Rice Pollen WEN Li,LIU Gai,LI Shao-qing,LI Guo-min,TAO Jun,ZHU Ying-guo 2007, 21(1): 13-19 .  Abstract ( )   PDF (3749KB) ( )   To better understand the molecular mechanism of cytoplasmic male sterility (CMS) at the protein level during the development of microspores, proteomic technique was used to investigate the protein expression profile in uninucleate-stage pollen of the rice CMS lines. Total pollen proteins were extracted using two different methods and then separated by two-dimensional gel electrophoresis (2-DE). An efficient and economic method for high quality protein preparation from rice pollen for 2-DE was established. The silver-stained protein spots were analyzed by using software PDQuest 2DE, there were 1 500 detectable spots on the gel. These results showed that, the proteins extracted by this method were in good integrity and purity, and could meet the needs of proteomic analysis. Comparing with the 2-DE maps of the total proteins from the binucleate-stage and uninucleate-stage pollen, the number of total proteins and the volume of some proteins were decreased in the binucleate-stage pollen.

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Relationship Between the First Base of Splice Donor of Wx Gene Intron 1 and Amylose Content in Yunnan Indigenous Rice Varieties ZHANG Ya-li,XU Ming-hui,ZENG Ya-wen,YAO Chun-xin,CHEN Shan-na 2007, 21(1): 20-24 .  Abstract ( )   PDF (392KB) ( )   There is a single nucleotide polymorphism, G or T, at the first base of splice donor site of Wx gene intron 1. A one-step PCR method was used to determine whether it is G or T in 220 Yunnan indigenous rice varieties from 14 districts, 55 towns/counties of Yunnan Province, of which 101 varieties were validated by PCR-AccⅠ method. According to the G/T polymorphism, 164 rice varieties showed GG genotype, while the other 56 were TT genotype, accounting for 74.5% and 25.5% of all the varieties, respectively. When all the rice varieties were divided into indica and japonica subspecies, it was found that 80.5% of indica rice and 67.0% of japonica rice showed GG genotype. The rice varieties with GG genotype had significantly higher amylose content (mean 18.97%) than those with TT genotype (all below 16%), though 33 rice varieties with GG genotype still had low amylose content ranging from 3.91% to 15.93%, but most of them came from the Dai minority area in the Southwest of Yunnan Province. However, there was no significant difference in the mean amylose content of the same GG or TT genotypes between indica and japonica rice, indicating that different genetic background, indica or japonica, had no effect on amylose content. The coefficient of correlation between genotype and amylose content was 0.733 (P<0.01).

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Marker-Assisted Selection of ZmC4Ppc Transformed Rice and Their Performance of Yield Components XIANG Xun-chao ,LI Ji-hang,HE Li-bin ,LI Ping 2007, 21(1): 25-30 .  Abstract ( )   PDF (280KB) ( )   The full-length of intact ZmC4Ppc gene is 6781 bp, and it’s difficult to obtain clear bands amplified by PCR, which affected the accuracy of marker-assisted selection (MAS) both in rice and maize. Specific fragments presented only in maize rather than in rice were identified and used for primer design using Primer Premier 5.0. A pair of specific primer termed MRpc (forward: 5′ AAGCAGGGAAGCGAGACG 3′, reverse: 5′ GATTGCCGCCAGCAGTAG 3′) was used for selection of transformed rice and ZmC4Ppc gene could be highly consitutive expressed at every developmental stage in rice. MRpc was used for MAS of progenies carrying ZmC4Ppc gene in rice and a transformed rice line FPM881 was gained, which was tested for analyzing the genetic background, PEPCase activity, net photosynthetic rate (Pn), general combining ability (GCA) and specific combining ability (SCA). The results indicated that the FPM881 showed an above 95% similar genetic background, a higher expression of PEPCase and an improved net photosynthetic rate than those of control. Analysis of yield components showed that some of the progenies carrying the ZmC4Ppc gene had better GCA and SCA for grain yield per plant, number of panicles per plant, and 1000-grain weight compared to the control. It suggested that the introduction of maize ZmC4Ppc gene via MAS with stable expression could increase rice grain yield and would likely provide a pathway for rice improvement.

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Breeding of Modified Single Cross Hybrid of indica Rice ZHOU Xue-biao,LU Zuo-mei 2007, 21(1): 31-38 .  Abstract ( )   PDF (52KB) ( )   Three full-sibling binary cytoplasmic male sterility(CMS) lines, 11 back-cross binary CMS lines, 10 half-sibling binary CMS lines, 10 CMS lines and 5 restorer lines Nanhui 04, Minghui 86, Fuhui 838, Xianhui 207 and Milyang 46 were chosen to make 50 single cross hybrids(SHs) and 120 modified single cross hybrids(MSHs) by the way of NCⅡ design. The grain yields of three types (full-sibling type, bock-cross type, half-sibling type) of MSH rice were higher than SHs on average. Among all the MSHs, 74 of them had superiority to the two corresponding SHs in grain yield, including 9 full-sibling type MSHs, 37 back-cross type MSHs and 28 half-sibling type MSHs. The coefficient of variability of the three types of MSHs were similar to single cross hybrids in plant height, flag-leaf length, flag-leaf width, number of effective tillers per plant and panicle length of main culms on average. The three types of MSHs were a little larger than the single cross hybrids in the days from 10% to 80% plants...ding in a plot, the days from the first plant heading to the latest plant heading in a plot, and the coefficient of variability of days from sowing to initial heading. A total of 10 good MSHs were selected according to the grain yield and the population uniformity, and its grain yield increased from 10.43% to 26.39% compared with the corresponding higher SHs.

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Mapping of Quantitative Trait Loci for Six Agronomic Traits of Rice in Pei′ai 64S/Nipponbare F2 Population YE Shao-ping,ZHANG Qi-jun,LI Jie-qin,ZHAO Bing,YIN De-suo,LI Ping 2007, 21(1): 39-43 .  Abstract ( )   PDF (682KB) ( )   Quantitative trait loci (QTL) for six related agronomic traits including tiller number per plant, effective tiller number per plant, percentage of tillering, plant height, flag leaf length and panicle length were mapped, by using a F2 population consisted of 180 lines from the cross between two genome-sequenced rice varieties Pei′ai 64S and Nipponbare, and a genetic linkage map including 137 SSR markers constituted with (Pei′ai 64S/Nipponbare) F2 population. A total of 14 QTLs for the six traits were identified at 11 intervals of chromosomes 1, 2, 4, 5, 6, and 7. One major QTL(qPH1-2) for plant height was located on chromosome 1, and another major QTL(qFL4) for flag leaf length, on chromosome 4. Moreover, the worth of the mapped QTLs and the distribution of QTL on chromosomes were discussed.

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Impacts of Amylose Content or Protein Content on Genetic Correlations Between the Weight of Brown Rice and Appearance Quality Traits of Rice GE Guo-ke,ZHANG Zhi,SHI Chun-hai,WU Jian-guo,YE Zi-hong 2007, 21(1): 44-50 .  Abstract ( )   PDF (89KB) ( )   A factorial mating design in two environments was conducted by using seven cytoplasm male sterile lines (A) and five restorer lines (R) along with their F1 (A×R) and F2. The unconditional and conditional genetic models and the corresponding statistic methods, including endosperm, cytoplasmic and maternal plant genetic effects, were used for revealing the influences of amylose content (AC) and protein content (PC) of rice to the genetic relationships between the weight of brown rice (WBR) and the appearance quality traits of indica rice (Oryza sativa L.). There were significant relationships between WBR and AC or PC of rice, and the conditional analysis method could be used to exclude the influences of AC and PC to WBR for revealing the real genetic relationships of the pairwise traits. The results from the conditional analysis indicated that AC could positively increase the genetic correlations between WBR and the brown rice length (BRL), the brown rice width (BRW), and the brown rice thickness (BRT), but decrease the genetic correlations between WBR and the ratio of brown rice length to width (RLW) and the ratio of brown rice length to thickness (RLT). PC could decrease the genetic correlations between WBR and BRL, BRW or BRT, and enhance the genetic correlation between WBR and RLW, but there was no visible influence for the genetic correlation between WBR and RLT. After excluding the influence of AC or PC to BRW, the correlation components from the different genetic systems showed that the visible differences could be found between WBR and the appearance quality traits of rice, especially for the cytoplasmic and the maternal additive main effects and their genotype×environment (GE) interaction effects, when compared with the results from the unconditional analysis.

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Evaluating Parameters of Rice Core Collections Based on Genotypic Values and Molecular Marker Information WANG Jian-cheng,HU Jin*, ZHANG Cai-fang,XU Hai-ming,ZHANG Sheng 2007, 21(1): 51-58 .  Abstract ( )   PDF (179KB) ( )   Monte Carlo simulation combining with mixed linear model were used in the research of evaluating parameters for rice core collection based on genotypic values and molecular marker information, which eliminated the interference of environment and obtained more reliable results. The coincidence rate of range (CR) was the optimal evaluating parameter. Mean Simpson index (MD), mean Shannon-Weaver index of genetic diversity (MI) and mean polymorphism information content (MPIC) were important evaluating parameters. The variable rate of coefficient of variation (VR) could act as an important referential parameter for evaluating the variation degree of core collection. Percentage of polymorphic loci (p) could act as a determination parameter for the size of core collection. Mean difference percentage (MD) was a determination parameter for the reliability judgement of core collection. The effective evaluating parameters for core collection selected by present research could be used in different plant germplasm population as criteria for sampling percentage.

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Variation of Starch Viscosity Characteristics (RVA Profile) of Hybrid Rice Liangyoupeijiu as Affected by Latitude and Seeding Time SHEN Xin-ping*,GU Li,SHEN Xiao-yan,GONG Li-ping,ZHANG Hong-cheng 2007, 21(1): 59-64 .  Abstract ( )   PDF (163KB) ( )   By setting up five seeding-time treatments at five different latitude zones in four Jiangsu rice growing regions(the latitude ranges from 31.3° N to 34.7° N), the RVA profile variation characteristic of the middle maturity two-line hybrid rice Liangyoupeijiu with good quality and high yield was studied. The RVA profile of Liangyoupeijiu was easily influenced by environmental factors. It was noted that at a latitude less than 32.4°, the variety possesed the best RVA profile, such as higher peak viscosity and lower setback value and pasting temperature. Moreover, from the seeding-time experiments, it was found that the RVA profile of Liangyoupeijiu worsened as the seeding was postponed, and the peaking viscosity would markedly increase and setback value would decrease with the increase of average temperature during the seeding period. Therefore, Liangyoupeijiu should be planted as early as possible for improving its grain quality.

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Relationship Between Leaf Nitrogen Content and Fluorescence Parameters in Rice MA Ji-feng,ZHU Yan,YAO Xia,TIAN Yong-chao,LIU Xiao-jun,CAO Wei-xing 2007, 21(1): 65-70 .  Abstract ( )   PDF (98KB) ( )   The fluorescence parameters of the 1st, 2nd, 3rd and 4th leaves from the top of culms in two rice varieties (Huajing 2 and Wuxiangjing 9) were measured with FMS-2 at different growth stages under three nitrogen levels, and the leaf nitrogen contents were correspondingly determined by destructive sampling and chemical assay. The leaf nitrogen contents and fluorescence parameters changed significantly with nitrogen levels, tending to increase with the nitrogen application rates. The fluorescence parameters exhibited a parabolic shape over the growth progress, initially increased until heading and then decreased to the lowest from heading to maturity stages. The distributions of fluorescence parameters on main culm in all treatments were L1>L2>L3>L4 (L1, L2, L3 and L4 mean the 1st, 2nd, 3rd and 4th leaf from the top). For the same variety, leaf nitrogen contents at different leaf positions were significantly correlated to fluorescence parameters, with better performance in Huajing 2 than in Wuxiangjing 9. With combined rice varieties, leaf nitrogen contents at different leaf positions were linearly related to fluorescence parameters ΦPSⅡ and qP. Different regression equations should be used to estimate leaf nitrogen contents at different leaf positions with ΦPSⅡ and qP. The results indicated that fluorescence parameters ΦPSⅡ and qP could be potentially useful for estimating leaf nitrogen content in rice.

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Effects of Interplanting with Zero Tillage and Wheat Straw Manuring on Rice Growth and Grain Quality LIU Shi-ping,NIE Xin-tao, DAI Qi-gen,HUO Zhong-yang,XU Ke 2007, 21(1): 71-76 .  Abstract ( )   PDF (40KB) ( )   Effects of interplanting with zero tillage and wheat straw manuring(ZIS) on rice growth and grain quality were studied in a wheat-rice cropping system. Results showed that ZIS reduced the plant height,leaf area per tiller and the biomass of rice, but the biomass accumulation of rice at the late stage was quicker than that under the transplanting cultivation. In the first year, the grain yield was the highest in ZIS, and the yield in transplanting with plowing and wheat straw manuring was higher than that without straw manuring. However, grain yield decreased in interplanting with zero tillage in the second year. Compared with other treatments with the similar number of panicles per unit area, the number of filled grains per panicle was the lowest but 1000-grain weight was the highest in interplanting with zero tillage, which was the main factor to higher yield. Interplanting with zero tillage improved the milling and appearance grain quality of rice. The rates of brown, milled and head rice increased while chalky rice rate and chalkiness decreased in interplanting with zero tillage. Transplanting with plowing and straw manuring also increased the head rice rate and slightly reduced the chalky rice rate and chalkiness. The cooking quality of rice in ZIS was improved due to the increase of protein, decline of amylose content and increase of gel consistency, while transplanting with straw manuring increased both the protein and amylose contents and decreased the gel consistency, which resulted in the decline of cooked rice quality.

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Relationships of Ethylene Evolution Rate and 1-Aminocylopropane-1-Carboxylic Acid Concentration in Grains During Grain Filling with Appearance Quality of Rice YANG Jian-chang*,CHANG Er-hua,TANG Cheng,ZHANG Hao,WANG Zhi-qin 2007, 21(1): 77-83 .  Abstract ( )   PDF (1181KB) ( )   To elucidate the relationship between ethylene evolution from the grains and the appearance quality of rice, ten rice genotypes were used to deterimine the ethylene evolution rate, 1-aminocylopropane-1-carboxylic acid (ACC) concentration and appearance quality of rice, and the effects of chemical regulators on concentrations of ethylene and ACC were also investigated to verify the roles of ethylene in rice quality. The ethylene evolution rates and ACC concentrations in grains during the mid and late grain filling stages were very significantly and positively correlated with chalky kernel percentage and chalkiness of rice. The varieties with a low ACC concentration in grains exhibited a close amyloplast arrangement and small gap between starch granules, whereas those with a high ACC concentration in the grains showed a loose arrangement and wide gap between the granules. Application of 1 μmol/L ACC to panicles at mid and late grain filling stages significantly loosened amyloplast arrangement and increased chalky kernel percentage, chalky area and chalkiness of rice, and the results were reversed when 1 μmol/L amino-ethoxyvinylglycine, an inhibitor of ACC synthetic enzyme, was applied to panicles. A practice of moderate dry-wet alternate irrigation reduced ethylene evolution and ACC concentration in grains and thereby reduced chalkiness. The results suggested that ethylene and ACC in grains played an important role in the endosperm structure and appearance quality of rice, and the appearance quality would be improved by reducing ethylene and ACC concentrations in grains either through variety breeding, or chemical regulations or cultivation techniques.

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Comparison of Allelopathy Potential Between an Exotic Invasive Weed Alternanthera philoxeroides and a Local Weed A. sessilis YU Liu-qing,Yoshiharu FUJII ,ZHOU Yong-jun, ZHANG Jian-ping,LU Yong-liang,XUAN Song-nan 2007, 21(1): 84-89 .  Abstract ( )   PDF (36KB) ( )   To estimate the allelopathy potential of Alternanthera philoxeroides, the inhibition rates of different organs of the exotic invasive weed A. philoxeroides on seedlings of lettuce (Lactuca sativa L.) and barnyardgrass [Echinochloa crusgalli (L.) Beauv. var. mitis (Pursh) Peterm.] were measured with a Chinese native weed A. sessilis as a control by the sandwich method. Under the treatment of 50 mg leaves of A. philoxeroides, the root growth of the lettuce and barnyardgrass was inhibited by 81% and 51%, respectively, and for the shoot growth by 49% and 48%, respectively, significantly higher than the control. However, there were no significant difference compared with that of the treatment of A. sessilis. The inhibition rate on the root length of barnyardgrass was 54% on average under the treatment of the root of A. philoxeroides, much higher than that of the treatments of its stem and leaf. In addition, with the dose of sample of A. philoxeroides increasing, the inhibition rate against the root growth of lettuce enhanced significantly, and the values were 54%, 61% and 83% under the treatments of 10 mg, 30 mg and 50 mg leaf of A. philoxeroides, respectively.

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Prospect and Practice of Three Procedure Breeding on Super Hybrid Rice CHEN Li-yun,XIAO Ying-hui,TANG Wen-bang,LEI Dong-yang 2007, 21(1): 90-94 .  Abstract ( )   PDF (37KB) ( )   The status of super hybrid rice breeding program in China and other countries were both reviewed. There were three main problems in super rice breeding in China, including: 1)the scarcity of super rice varieties, 2) poor adaptation of super hybrid rice combination, 3)current breeding theories put too much emphasis on the rice growth model, and lack in practicability for guiding rice breeding. According to the author′s experience on rice breeding, the breeding strategy, which involved in three procedures, were proposed. The objective of each procedure and the key technologies for achieving the goals were elucidated in detail. The super parent of hybrid rice should exhibit a comprehensive excellent performance, with the yield or sink capacity equal to the hybrid rice control in regional yield trial. The super hybrid rice combination should meet the following criteria, including high rice quality, wide adaptation, lodging resistance, main insects and diseases resistance, and a yield increase above 8% compared with the control varieties in regional yield trial. Through optimal combination of the parents, increasing selection pressure, and paying more attention to harmony on ideal type, excellent physiological function and all the agronomic traits, the goals of super hybrid rice should be achieved. The yield of hybrid rice seed production should reach 3.75 t/hm2 and 5.25 t/hm2 for early-season and middle-season type of male sterile line, respectively. The main technologies included selection of optimal ecological site, use of the male sterile line with large sink capacity and good outcrossing characteristics, and increased amount of the pollen by intensive cultivation of the male parent. According to the breeding technologies, two super rice parents, including a male parent 996 and a T(P)GMS C815S, were bred. The super hybrid rice combination, Luliangyou 996, which can be used as a double-season early rice variety in middle and lower reaches of Changjiang River, China, was bred by using the super rice variety 996 as the male parent. Several hybrid rice combinations, with higher yield than control varieties both in Hunan provincial and national regional yield trials, were bred with the T(P)GMS C815S as the male parent.

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Analysis of Quantitative Trait Loci for Resistance to Stripe Disease in Rice SUN Dai-zhen,JIANG Ling,ZHANG Ying-xin,CHENG Xia-nian,ZHAI Hu-qu,WAN Jian-min 2007, 21(1): 95-98 .  Abstract ( )   PDF (89KB) ( )   In order to verify the quantitative trait loci for the resistance to rice stripe in rice variety Zhaiyeqing 8, a molecular linkage map was constructed based on the F2 population derived from the cross of Zhaiyeqing 8 and Wuyujing 3. Reactions of the two parents, F1 individuals and 129 F2∶3 lines to the rice stripe were investigated by both artificial inoculation at laboratory and natural infection methods in the field attached the virus, and the ratio of disease rating index were scored. The ratios of disease rating index in Zhaiyeqing 8/Wuyujing 3 F2∶3 population ranged from 0 to 134.08 and from 6.25 to 133.6 by artificial inoculation at laboratory and natural infection methods, respectively, with marked bias towards resistant parent, Zhaiyeqing 8. This indicates the resistance to the rice stripe was controlled by quantitative trait loci (QTL). Further QTL analysis showed that the QTLs detected by two inoculation methods were completely different. Only one QTL, qSTV7,was detected by artificial inoculation, at which the Zhaiyeqing 8 allele enhanced the resistance to rice stripe, while the other two QTLs, qSTV5 and qSTV1, were detected by natural infection, and the resistant alleles came from Zhaiyeqing 8 and Wuyujing 3, respectively. These results showed that the resistant parent Zhaiyeqing 8 carried the genes associated with the resistance to the rice stripe virus and small brown planthopper, the resistant gene in Wuyujing 3 was expressed after recombination between Wuyujing 3 and Zhaiyeqing 8. In comparison with the results previously reported, QTLs detected in the study were new resistant genes to rice stripe. This will provide a new resistant resource for avoiding genetic vulnerability for single resistant gene, Stvb-i.

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Relationship Between Canopy Temperature and Soil Water Content, Yield Components at Flowering Stage in Rice ZHANG Wen-zhong,HAN Ya-dong,DU Hong-juan,HUANG Rui-dong,CHEN Wen-fu 2007, 21(1): 99-102 .  Abstract ( )   PDF (150KB) ( )   The canopy temperature of rice at the flowering stage and the soil water content were investigated under different soil water treatments. It showed that the canopy temperature was lower than the air temperature, while the soil water content significantly influenced the canopy temperature, the lower the soil water content, the higher the canopy temperature, the less the accumulative absolute value of canopy-air temperature difference. Moreover, the maximum difference between treatments and CK in the accumulative absolute value of canopy-air temperature appeared at 13:00 pm, therefore, it could be considered as a suitable measuring time. It was noted that the peak flowering time of rice was ahead under the lowest water content treatment, and the peak flowering time was concentrated on the first three days, resulting in shortened and lightened the panicle of rice. As to the CK and the high water content treatments, the peak flowering time was concentrated at the middle of flowering duration, with longer panicle length and higher panicle weight. The results indicated the lower the soil water content, the less the full filled grain number.

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Changes of Activity of Glutamine Synthetase During Grain Filling and Its Influence on Rice Grain Quality JIN Zheng-xun,QIAN Chun-rong,YANG Jing,LIU Hai-ying 2007, 21(1): 103-106 .  Abstract ( )   PDF (84KB) ( )   Changes of Activity of Glutamine Synthetase During Grain Filling and Its Influence on Rice Grain Quality

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Analysis on Variation of Tested Values for Grain Quality of indica Hybrid Rice MIN Jie,ZHANG Bo-ping,ZHU Zhi-wei,DUAN Bin-wu,ZHANG Lin-ping 2007, 21(1): 107-110 .  Abstract ( )   PDF (31KB) ( )   The variation and stability of the tested values of 12 indices for grain quality were analyzed by using 1036 indica hybrid rice samples from different sites and years. The results indicated that: 1) Under the comprehensive effects of genetic combination, environment and test technology, the brown rice rate, milled rice rate, grain length and length/width ratio showed high stability (CV < 10%); and the protein content, amylose content, gelatinization temperature, head rice rate and gel consistency showed medium stability (10%≤CV≤25%);but the rice translucency, chalky rice rate and chalkiness degree showed low stability (CV>25%). 2) In case of removing genetic effect, the CV of all tested values decreased at various degrees but the order of CV remained unchanged. 3) In case of removing both effects of genetics and environment, the CV of all tested values reduced significantly, showing the influence of test technology and skillability on the CV of tested values. The factors causing variation of the tested values and the issue on how to increase testing stability for rice quality analysis were discussed.