中国水稻科学
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  2007, Vol. 21 Issue (1): 20-24     DOI:
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Relationship Between the First Base of Splice Donor of Wx Gene Intron 1 and Amylose Content in Yunnan Indigenous Rice Varieties
ZHANG Ya-li; XU Ming-hui;ZENG Ya-wen;YAO Chun-xin;CHEN Shan-na
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Abstract There is a single nucleotide polymorphism, G or T, at the first base of splice donor site of Wx gene intron 1. A one-step PCR method was used to determine whether it is G or T in 220 Yunnan indigenous rice varieties from 14 districts, 55 towns/counties of Yunnan Province, of which 101 varieties were validated by PCR-AccⅠ method. According to the G/T polymorphism, 164 rice varieties showed GG genotype, while the other 56 were TT genotype, accounting for 74.5% and 25.5% of all the varieties, respectively. When all the rice varieties were divided into indica and japonica subspecies, it was found that 80.5% of indica rice and 67.0% of japonica rice showed GG genotype. The rice varieties with GG genotype had significantly higher amylose content (mean 18.97%) than those with TT genotype (all below 16%), though 33 rice varieties with GG genotype still had low amylose content ranging from 3.91% to 15.93%, but most of them came from the Dai minority area in the Southwest of Yunnan Province. However, there was no significant difference in the mean amylose content of the same GG or TT genotypes between indica and japonica rice, indicating that different genetic background, indica or japonica, had no effect on amylose content. The coefficient of correlation between genotype and amylose content was 0.733 (P<0.01).
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ZHANG Ya-li
XU Ming-hui
ZENG Ya-wen
YAO Chun-xin
CHEN Shan-na
Key wordsYunnan   indigenous rice variety   waxy gene   intron   base   amylose content     
Received: 1900-01-01;
Cite this article:   
ZHANG Ya-li,XU Ming-hui,ZENG Ya-wen et al. Relationship Between the First Base of Splice Donor of Wx Gene Intron 1 and Amylose Content in Yunnan Indigenous Rice Varieties[J]. , 2007, 21(1): 20-24 .
 
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