Abstract: PCR analysis on 53 ferritin transgenic plants showed that 52 T₁ transgenic plants existed a pea ferritin gene. Photosynthetic function of the transgenics with 60 μmol/L paraquat treatment was determined by measurement of maximum photochemical efficiency (F_v/F_m value) and chlorophyll content. Results indicated that transgenic plants exhibited a various degree of tolerance to oxidative stress. Accumulation of mRNA of Fer gene was demonstrated by Northern blot analysis. Results showed the accumulation of mRNA level was elevated in 5 of 9 plants determined. T₂ progeny of transgenic plants showed resistance to Magnaporthe grisea comparing with the non transgenic plants. The average number of lesion in transgenic plants leaves inoculated with Magnaporthe grisea was decreased significantly. These results suggested the overexpression of Fer gene in rice enhanced its resistance to oxidative damage and Magnaporthe grisea infection.

Key words: Oryza sativa, ferritin, oxidative damage, Magnaporthe grisea, tolerance

摘要： 对转豌豆铁蛋白（pea ferritin，Fer）基因水稻T1代的53个株系进行PCR检测，52个株系能扩增出阳性PCR产物。通过测定光合作用过程中最大光化学通量（Fv/Fm值）分析了由百草枯处理引起的T1代水稻叶片的氧化损害。与未转基因水稻相比，转Fer基因水稻的叶片对氧化胁迫的耐受能力有不同程度的增强。百草枯处理后转基因植株叶片叶绿素含量与水处理叶片相比没有明显下降，而未转基因植株叶片叶绿素含量降低至水处理叶片的20％左右。选取9株对氧化胁迫耐受能力较强的水稻进行了Northern blot分析和子代的稻瘟病抗性测定，其中5株转基因植株Fer mRNA 积累增强。病原菌接种后T2代转基因植株的病斑数量明显少于非转基因植株。表明转Fer基因水稻对氧化胁迫和病原菌有较好的抗性。

关键词: 水稻, 铁蛋白, 氧化损害, 稻瘟病菌, 抗性