中国水稻科学
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  2004, Vol. 18 Issue (4): 357-361     DOI:
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Screening of Temperature Sensitive Mutants in Magnaporthe grisea
WANG Hong-kai 1; LIN Fu-cheng 2; CHAI Rong-yao 3; LI De-bao 1
1 Biotechnology Institute; Zhejiang University; Hangzhou 310029; China; 2 College of Life Sciences; 3 Institute of Plant Protection and Microbiology; Zhejiang Academy of Agricultural Sciences; Hangzhou 310021; China)
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Abstract Temperature sensitive mutants(ts mutants) are good material for studying cell division and differentiation. Two hundred and seventy four ts mutants of Magnaporthe grisea were induced by UV radiation. These mutants could grow at 25℃, but not at 33℃. Pathogenicity test indicated that the pathogenicity of 32 ts mutants reduced distinctly. Under 33℃ conditions, seven of them did not germinate, 12 stopped growing after germination and no appressorium formed, nine stopped growing after appressorium formed, four mutants formed swell and bunch appressorium, then stopped growing. The ability of perithecia formation of some mutants was changed.
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WANG Hong-kai
LIN Fu-cheng
CHAI Rong-yao
LI De-bao
Key wordsMagnaporthe grisea   temperature sensitive mutant   germination   appressorium   perithecia     
Received: 1900-01-01;
Cite this article:   
WANG Hong-kai ,LIN Fu-cheng ,CHAI Rong-yao et al. Screening of Temperature Sensitive Mutants in Magnaporthe grisea[J]. , 2004, 18(4): 357-361 .
 
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