Abstract: The genes encoding enzymes responsible for synthesizing some low-molecular weight metabolites, such as betaine, polyamines, and mannitol,were transformed into rice plants to enhance the salt- tolerance. The improvement of rice salt- tolerance with five target genes (BADH, CMO, mtlD, gutD and SAMDC) was conducted through gene transformation and traditional breeding approaches. Results indicated that the genes were successfully transformed into rice by particle bombardment or mediated by Agrobacterium tumefaciens and integrated into the genome of the transgenic plants by using PCR detections. The salt tolerance of the transgenic plants and their progenies with 1-5 genes was significantly enhanced and stably maintained in the salt-stress fields. Nine elite rice transgenic lines such as Pin 3, Pin 6 and Pin 7 from Xiushui 11 were obtained, which grew normally in the salt pool containing 0.5%-1.0% NaCl.

Key words: genes related to salt-tolerance, transformation, traditional breeding, elite germplasm, rice (Oryza sativa)

摘要： 通过农杆菌介导法和基因枪法将CMO、BADH、mtlD、gutD和SAMDC基因以单价或双价的形式导入水稻常规品种中，再结合常规杂交育种，选育5价强耐盐性的转基因水稻植株。1~5价9类组合的水稻品系，经PCR分子检测，在转基因后代中多价目的基因聚合，遗传稳定，且分子检测与田间耐盐性的表现一致。转基因植株在盐碱地中能正常生长，拓展了水稻常规品种耐盐性。并已获得耐0.5%~1.0%NaCl的T秀水11——品3、品6和品7等9份优良株系或中间材料。

关键词: 耐盐相关基因, 遗传转化, 常规杂交, 新种质