Postanthesis Changes in Activities of Enzymes Related to Starch Synthesis and Contents of Hormones in Superior and Inferior Spikelets and Their Relation with Grain Filling of Super Rice

doi:10.3969/j.issn.10017216.2012.03.008

Abstract

Abstract: The changes in activities of key enzymes involved in sucrosetostarch conversion and contents of hormones in superior and inferior spikelets were investigated and their relation with grain filling of super rice was analyzed with four super rice cultivars, Liangyoupeijiu and Ⅱyou 084 (indica hybrids), Huaidao 9 and Wujing 15 (japonica), and two highyielding and elite check cultivars, Shanyou 63 (indica hybrid) and Yangfujing 8 (japonica)as material in paddy field. Activities of sucrose synthase (SuSase), adenosine diphosphoglucose pyrophosphorylase (AGPase), starch synthase (StSase), and starch branching enzyme (SBE) and contents of zeatin + zeatin riboside (Z+ZR), indole3acetic acid (IAA), abscisic acid (ABA) in superior and inferior spikelets were determined during the grain filling period and their relationships with grain filling rate were analyzed. Maximum grain filling rate, the time reaching a maximum grainfilling rate, mean grain filling rate and brown rice weight for superior spikelets slightly differed between super rice and elite check rice, but those for inferior spikelets were significantly different between super rice and check rice cultivars. Changes in enzyme activity and contents of hormones in grains followed single peak curves during the grain filling period. The peak and mean activity of SuSase, AGPase, StSase and SBE were lower in inferior spikelets than in superior ones,as well as the peak and mean contents of Z+ZR, IAA and ABA. The peak and mean contents of ABA were significantly greater in inferior spikelets than in superior ones and greater in the super rice than in the check rice. The grain filling rate was positively significantly correlated with the activities of SuSase, AGPase and StSase and contents of Z+ZR and IAA, but not significantly correlated with activity of SBE and ABA contents. The results suggested that the low activities of SuSase, AGPase and StSase and low contents of Z+ZR and IAA may be an important reason for the slow grain filling rate and low grain weight of inferior spikelets in super rice.

Key words: super rice, superior spikelet, inferior spikelet, enzyme activity, hormone, grain filling

摘要： 种植4个超级稻品种\[两优培九和Ⅱ优084（杂交籼稻）、淮稻9号和武粳15（粳稻）\]和2个高产对照品种\[汕优63（杂交籼稻）和扬辐粳8号（粳稻）\]，观察其结实期强、弱势粒中蔗糖合酶（SuSase）、腺苷二磷酸葡萄糖焦磷酸化酶（AGPase）、淀粉合酶（StSase）和淀粉分支酶（SBE）活性及玉米素+玉米素核苷（Z+ZR）、3吲哚乙酸（IAA）和脱落酸（ABA）含量的变化，并测定了强、弱势粒灌浆速率。结果表明，超级稻品种强势粒的最大灌浆速率、到达最大灌浆速率的时间、平均灌浆速率和糙米重与对照品种差异较小，超级稻品种弱势粒的灌浆速率和糙米重显著低于对照。灌浆期强、弱势粒的SuSase、AGPase 、StSase 和SBE活性变化及Z+ZR、IAA 和ABA 含量变化均呈单峰曲线。弱势粒的SuSase、AGPase、StSase 和SBE的峰值活性和平均活性及其Z+ZR 和IAA的峰值含量和平均含量均低于强势粒。弱势粒的ABA 峰值含量和平均含量显著高于强势粒，超级稻品种高于对照品种。籽粒灌浆速率与SuSase、AGPase和StSase 活性及Z+ZR 和IAA含量均呈显著或极显著正相关，与SBE活性及ABA含量的相关不显著。说明超级稻品种弱势粒中较低的SuSase、AGPase和StSase活性及较低的Z+ZR和IAA含量是其灌浆速率小、粒重轻的一个重要原因。

关键词: 超级稻, 强势粒, 弱势粒, 酶活性, 激素, 籽粒灌浆

CLC Number:

FU Jing, XU Yunji, CHEN Lu, YUAN Limin, WANG Zhiqin, YANG Jianchang*. Postanthesis Changes in Activities of Enzymes Related to Starch Synthesis and Contents of Hormones in Superior and Inferior Spikelets and Their Relation with Grain Filling of Super Rice[J]. Chinese Journal of Rice Science, 2012, 26(3): 302-310.

付景,徐云姬,陈露,袁莉民,王志琴,杨建昌*. 超级稻花后强、弱势粒淀粉合成相关酶活性和激素含量变化及其与籽粒灌浆的关系[J]. 中国水稻科学, 2012, 26(3): 302-310.

References

\[1\]Kato T, Takeda K. Associations among characters related to yield sink capacity in spaceplanted rice. Crop Sci,1996, 36: 11351139.

\[2\]Kato T, Shinmura D, Taniguchi A. Activities of enzymes for sucrosestarch conversion in developing endosperm of rice and their association with grain filling in extraheavy panicle types.Plant Prod Sci, 2007, 10: 442450.

\[3\]Peng S B, Cassman K G, Virmani S S, et al. Yield potential trends of tropical since the release of IR8 and its challenge of increasing rice yield potential.Crop Sci, 1999, 39: 15521559.

\[4\]Cheng S H, Zhuang J Y, Fan Y Y, et al. Progress in research and development on hybrid rice: A superdomesticate in China. Ann Bot, 2007, 100: 959966.

\[5\]Peng S B, Khush G S, Virk P, et al. Progress in ideotype breeding to increase rice yield potential. Field Crops Res,2008, 108: 3238.

\[6\]敖和军, 王淑红, 邹应斌, 等. 超级杂交稻干物质生产特点与产量稳定性研究. 中国农业科学, 2008, 41(7): 19271936.

\[7\]Yang J C, Zhang J H. Grain filling problem in “super” rice. J Exp Bot,2010, 61: 15.

\[8\]Zhang H, Tan G L, Yang L N, et al. Hormones in the grains and roots in relation to postanthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiol Biochem,2009, 47: 195204.

\[9\]Mohapatra P K, Patel R, Sahu S K. Time of flowering affects grain quality and spikelet partitioning within the rice panicle.Aust J Plant Physiol, 1993, 20: 231242.

\[10\]Yang J C, Peng S B, Visperas R M, et al. Grain filling pattern and cytokinin content in the grains and roots of rice plants. Plant Growth Regul, 2000, 30: 261270.

\[11\]Liang J S, Zhang J H, Cao X Z. Grain sink strength may be related to the poor grain filling of indicajaponica rice (Oryza sativa) hybrids. Physiol Plantarum, 2001, 112: 470477.

\[12\]Yang J C, Zhang J H, Huang Z L, et al. Correlation of cytokinin levels in the endosperm and roots with cell number and cell division activity during endosperm development in rice. Ann Bot, 2002, 90: 369377.

\[13\]Nakamura Y, Yuki K. Changes in enzyme activities associated with carbohydrate metabolism during development of rice endosperm. Plant Sci, 1992, 82: 1520.

\[14\]Murata Y, Matsushima S. Rice//Evans L T, ed. Crop physiology. Cambridge: Cambridge University Press, 1975. 7599.

\[15\]Nakamura Y, Yuki K, Park S Y. Carbohydrate metabolism in the developing endosperm of rice grains. Plant Cell Physiol,1989, 30: 833839.

\[16\]Kato T. Change of sucrose synthase activity in developing endosperm of rice cultivars. Crop Sci,1995, 35: 827831.

\[17\]Ahmadi A, Baker D A. The effect of water stress on the activities of key regulatory enzymes of the sucrose to starch pathway in wheat. Plant Growth Regul, 2001, 35: 8191.

\[18\]Yang J C, Zhang J H, Wang Z Q, et al. Postanthesis development of inferior and superior spikelets in rice in relation to abscisic acid and ethylene.J Exp Bot, 2006, 57: 149160.

\[19\]Yang J C, Zhang J H, Wang Z Q, et al. Hormones in the grains in relation to sink strength and postanthesis development of spikelets in rice. Plant Growth Regul, 2003, 41: 185195.

\[20\]Richards F J. A flexible growth function for empirical use. J Exp Bot,1959, 10(29): 290300.

\[21\]朱庆森, 曹显祖, 骆亦其. 水稻籽粒灌浆的生长分析. 作物学报, 1988, 14(3): 182193.

\[22\]Yang J C, Zhang J H, Wang Z Q, et al. Activities of enzymes involved in sourcetostarch metabolism in rice grains subjected to water stress during filling. Field Crops Res, 2003, 81: 6981.

\[23\]程方民, 蒋德安, 吴平, 等. 早籼稻籽粒灌浆过程中淀粉合成酶的变化及温度效应特征. 作物学报, 2001, 27(2): 201206.

\[24\]陈远平, 杨文钰. 卵叶韭休眠芽中GA3、IAA、ABA和ZT的高效液相色谱法测定. 四川农业大学学报, 2005, 23(4): 498500.

\[25\]Yuan L P. Increasing yield potential in rice by exploitation of heterosis//Virmani S S.ed. Hybrid Rice Technology, New Developments and Future Prospects.Philippines: International Rice Research Institute, 1994: 16.

\[26\]Yuan L P. Hybrid rice breeding in China//Virmani S S, Sissiq E A, Muralidharan K.ed. Advances in Hybrid Rice Technology.Philippines: International Rice Research Institute, 1998: 2733.

\[27\]王志琴, 叶玉秀, 杨建昌, 等. 水稻灌浆期籽粒中蔗糖合成酶活性的变化与调节. 作物学报, 2004, 30(7): 634643.

\[28\]Preiss J. Biosynthesis of starch and its regulation//Preiss .ed. The Biochemistry of Plants. New York:Academic press 1988, 14: 181254.

\[29\]Okita T W. Is there an alternative pathway for starch synthesis?Plant physiol, 1992, 100: 560564.

\[30\]赵步洪, 张文杰, 常二华, 等. 水稻灌浆期籽粒中淀粉合成关键酶的活性变化及其与灌浆速率和蒸煮品质的关系. 中国农业科学, 2004, 37(8): 11231129.

\[31\]杨建昌, 王志琴, 朱庆森, 等. ABA与GA对水稻籽粒灌浆的调控. 作物学报, 1999, 25(3): 341348.

\[32\]Zhu G H, Ye N H, Yang J C, et al.Regulation of expression of starch synthesis genes by ethylene and ABA in relation to the development of rice inferior and superior spikelets. J Exp Bot, 2011, 62(11): 39073916.

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