中国水稻科学
     Home | About Journal | Editorial Board | Publication Ethics Statement | Subscriptions | Advertisement | Contacts Us | Chinese
  2011, Vol. 25 Issue (2): 206-202     DOI: 10.3969/j.issn.1001-7216.2011.02.014
研究报告 Current Issue | Next Issue | Archive | Adv Search  |   
Studies on Biological Characteristics and Pathogenicity Differentiation of Rice Sheath Blight Pathogen from Three Provinces in South China
ZOU Cheng-jia#, TANG Fang# , YANG Mei, HE Xiao-xia, LI Xian-jun, ZHOU Er-xun*
(College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China; #These authors contributed equally to this paper; *Corresponding author, E-mail: exzhou@scau.edu.cn)
 Download: PDF (499 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract In order to elucidate the relationship among the biological characteristics, pathogenicity and geographic origin of isolates of Rhizoctonia solani, the causal agent of rice sheath blight, so as to provide evidences for controlling the disease in South China, with a total of 335 isolates of R. solani collected from 33 counties of 3 provinces (Guangdong, Guangxi and Hainan) in South China, cultural characteristics (hyphal growth rate, colony colour and sclerotium number), hyphal anastomosis groups, number of hyphal cell nuclei and pathogenicity differentiation were investigated. The hyphal growth rates could be divided into three types, i.e. slow, moderate and fast, with isolate numbers of 3, 136 and 196, which accounted for 0.90%, 40.60% and 58.50% of the total isolates, respectively. The number of sclerotia produced on PDA medium in all the isolates could be divided into four different types, i.e. absence or rare, less, moderate and abundant sclerotia, which accounted for 1.19%, 17.61%, 71.04% and 10.15% of the total isolates, respectively. The anastomosis grouping confirmed that all the tested isolates belonged to AG-1ⅠA of R. solani. The hyphal cell nuclear numbers of the 50 randomly selected isolates were between 7 and 13. The 270 tested isolates could be divided into only two pathotypes, i.e. moderate and high virulence with isolate numbers of 208 and 62, which accounted for 77.04% and 22.96%, respectively, no isolate with weak virulence was found. Taken together, the results revealed that there existed differences in pathotype and hyphal growth rate as well as the number of sclerotia among the tested isolates, showing more diversity.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
ZOU Cheng-jia#
TANG Fang#
YANG Mei
HE Xiao-xia
LI Xian-jun
ZHOU Er-xun*
Key wordsRhizoctonia solani   cultural characteristics   hyphal anastomosis group   pathogenicity differentiation     
Received: 2010-04-01;
Cite this article:   
ZOU Cheng-jia#,TANG Fang# ,YANG Mei et al. Studies on Biological Characteristics and Pathogenicity Differentiation of Rice Sheath Blight Pathogen from Three Provinces in South China[J]. , 2011, 25(2): 206-202 .
 
[1] Lee F N, Rush M C. Rice sheath blight: A major rice disease. Plant Dis, 1983, 67(7): 829-832. [2]黄江华, 杨媚, 周而勋, 等. 13种植物丝核菌对水稻、甜玉米、黄瓜和甘蓝的交互致病性. 华中农业大学学报, 2008, 27 (2): 198-203. [3]Guleria S, Aggarwal R, Thind T S, et al. Morphological and pathological variability in rice isolates of Rhizoctonia solani and molecular analysis of their genetic variability. J Phytopathol, 2007, 155: 654-661. [4]Stodart B J, Harvey R, Neate S M, et al. Genetic variation and pathogenicity of anastomosis group 2 isolates of Rhizoctonia solani in Australia. Mycol Res, 2007, 111: 891-900. [5]Ogoshi A. Ecology and pathogenicity of anastomosis and intraspecific groups of Rhizoctonia solani Kühn. Annu Rev Phytopathol, 1987, 25: 125-143. [6]孟庆忠, 刘志恒, 王鹤影, 等. 水稻纹枯病研究进展. 沈阳农业大学学报, 2001, 32(5): 376-381. [7]周而勋, 曹菊香, 杨媚, 等. 我国南方六省 (区) 水稻纹枯病菌遗传多样性的研究. 南京农业大学学报, 2002, 25(3): 36-40. [8]周而勋, 杨媚, 陈友林. 土壤环境因素对水稻纹枯病菌腐生定殖能力的影响. 植物病理学报, 2002, 32(3): 214-218. [9]周而勋, 杨媚, 李琳, 等. 培养基对水稻纹枯病菌菌丝生长和菌核形成的影响. 华南农业大学学报:自然科学版, 2002, 23(3): 33-35. [10] 周而勋,曹菊香,杨媚. 水稻纹枯病组织病理学的研究进展. 仲恺农业技术学院学报, 2002, 15(2): 61-65. [11] 黄世文, 王玲, 陈惠哲, 等. 氮肥施用量和施用方法对超级杂交稻纹枯病发生的影响. 植物病理学报, 2009, 39(1): 104-109. [12] 陈夕军, 王玲, 左示敏, 等. 水稻纹枯病寄主-病原物互作鉴别品种与菌株的筛选. 植物病理学报, 2009, 39(5): 514-520. [13]Bernades-de-Assis J, Storari M, Zala J, et al. Genetic structure of populations of the rice-infecting pathogen Rhizoctonia solani AG-1 IA from China. Phytopathology, 2009, 99(9): 1090-1099. [14]殷跃军, 左示敏, 王辉, 等. 利用近等基因系研究3个抗水稻纹枯病QTL的聚合效应. 中国水稻科学, 2008, 22(4): 340-346. [15]Banniza S, Sya A, Bridge P D, et al. Characterization of populations of Rhizoctonia solani in paddy rice fields in Cte d’Ivoire. Phytopathology, 1999, 89: 414-420. [16]Taheri P, Gnanamanickam S, Hfte M. Characterization, genetic structure, and pathogenicity of Rhizoctonia spp. associated with rice sheath diseases in India. Phytopathology, 2007, 97: 373-383. [17] 周而勋, 杨媚. 从植物病组织中分离丝核菌的快速、简便技术. 华南农业大学学报, 1998, 19(1): 125-126. [18] 黄江华, 杨媚, 周而勋, 等. 广州地区10种作物立枯丝核菌的融合群鉴定. 仲恺农业技术学院学报, 2002, 15(1): 14-18. [19] 黄江华, 杨媚, 周而勋, 等. 丝核菌细胞核染色技术的研究. 仲恺农业技术学院学报, 2001, 14(4): 13-17. [20] 王子斌, 左示敏, 李刚, 等. 水稻抗纹枯病苗期快速鉴定技术研究. 植物病理学报, 2009, 39(2): 174-182. [21] 左示敏, 张亚芳, 殷跃军, 等. 田间水稻纹枯病抗性鉴定体系的确立与完善. 扬州大学学报:农业与生命科学版, 2006, 27 (4): 57-61. [22]Sneh B, Burpee L, Ogoshi A. Identification of Rhizoctonia species. St Paul, Minnesota: The American Phytopathological Society Press, 1991. [23]Kuninaga S, Yokosawa R. DNA base sequence homology in Rhizoctonia solani Kühn: I. Genetic relatedness within anastomosis group 1. Ann Phytopathol Soc Jpn, 1982, 48: 659-667. [24] 李华荣. 丝核菌 (Rhizoctonia) 属真菌的分类学进展. 国外农学—植物保护, 1988(4): 9-13. [25]Liu Z L, Sinclair J B. Differentiation of intraspecific groups within anastomosis group 1 of Rhizoctonia solani using ribosomal DNA internal transcribed spacer and isozyme comparison. Can J Plant Pathol, 1993, 15: 272-280. [26]Damicone J P, Patel M V, Moore W F. Density of sclerotia of Rhizoctonia solani and incidence of sheath blight in rice fields in Mississippi. Plant Dis, 1993, 77: 257-260. [27] 周而勋, 杨媚. 广东省水稻纹枯病菌的致病力和融合群研究. 广东农业科学, 1999(5): 36-38. [28] 赵长江, 胡伯里, 鲁国东, 等. 福建省水稻纹枯病菌的致病力及遗传多样性分析. 西北农林科技大学学报:自然科学版, 2005, 33(增): 60-64. [29]Toda T, Hyakumachi M, Suga H, et al. Differentiation of Rhizoctonia AG-D isolates from turfgrass into subgroups I and II based on rDNA and RAPD analyses. Eur J Plant Pathol, 1999, 105: 835-846. [30]Justesen A F, Yohalem D, Bay A, et al. Genetic diversity in potato field populations of Thanatephorus cucumeris AG-3, revealed by ITS polymorphism and RAPD markers. Mycol Res, 2003, 107(11): 1323-1331. [31] 陈涛, 张震, 柴荣耀, 等. 浙江省水稻纹枯病菌的遗传分化与致病力研究. 中国水稻科学, 2010, 24(1): 67-72.在线辅助信息表1
[1] WANG Ling,HUANG Wen-wen,HUANG Shi-wen,LIU Lian-meng,LIU En-yong,. Pathogenicity Differentiation of Rice Sheath Blight Pathogen Rhizoctonia solani AG-1 ⅠA Isolates from Anhui and Hubei Provinces, China[J]. , 2010, 24(6): 623-629 .
[2] CHEN Tao, ZHANG Zhen, CHAI Rong-yao,WANG Jiao-yu,MAO Xue-qin,QIU Hai-ping,DU Xin-fa,JIANG Hua, WANG Li-an,WANG Yan-li .

Genetic Diversity and Pathogenicity Variation of Different Rhizoctonia solani Isolates in Rice from Zhejiang Province, China

[J]. , 2010, 24(1): 67-72 .
[3] XIAO Yong ,LIU Ming-wei ,LI Gang ,ZHOU Er-xun ,WANG Ling-xia ,TANG Jie ,TAN Fu-rong ,ZHENG Ai-ping ,LI ping. Genetic Diversity and Pathogenicity Variation of Different Rhizoctonia solani Isolates in Rice from Sichuan Province, China[J]. , 2008, 22(1): 87-92 .
[4] HE Qing-fang,CHEN Wei-liang,MA Zhi-chao. Purification and Properties of Antagonistic Peptide Produced by Bacillus subtilis A30[J]. , 2002, 16(4): 361-365 .
Copyright © Chinese Journal of Rice Science 浙ICP备05004719号-5
Supported by: Beijing Magtech