Effects of Plant Hormones in Regulating Sucrose Synthase Activity and Grain Filling of Rice

doi:10.3969/j.issn.1001-7216.2011.02.010

Abstract

Abstract: In order to explore the mechanisms of hormones in the regulation of grain filling, the changes in contents and activities of enzymes which catalyze sucrose metabolism and endogenous hormones in grains during grain filling were analyzed by using inter-subspecies hybrid rice combination Liangyoupeijiu and intervarietal hybrid rice combination Shanyou 63 as materials. Results showed that superior grains had higher contents of endogenous indole-3-acetic acid (IAA), zeatin riboside (ZR) and abscisic acid (ABA) compared with inferior grains at early grain filling stage, and the contents of IAA, ZR and ABA were positively and significantly correlated with the maximum and mean grain filling rate, grain filling percentage, and grain weight. The sucrose synthase (SuSase) activity in superior grains was higher and peaked earlier than that in inferior grains. The grain filling rate, grain filling percentage, grain weight and starch accumulation rate were positively and significantly correlated with the SuSase activity in grains. Application of low concentration of ABA at 8 d after flowering significantly increased endogenous ABA content and SuSase activity in grains, grain filling percentage, and final grain weight. On the contrary, when fluridone (an inhibitor of ABA synthesis) was applied, the ABA content and the activity of SuSase decreased. These results suggest that ABA plays a significant regulatory role in grain filling largely through affecting sucrose synthase activity.

Key words: abscisic acid, grain filling, inter-subspecies hybrid rice, sucrose synthase

摘要：

以亚种间杂交稻两优培九和品种间杂交稻汕优63为材料，对籽粒灌浆期间的内源激素含量和酶活性进行了测定分析，并结合外源激素对水稻籽粒灌浆过程的调控试验，分析了脱落酸对蔗糖代谢关键酶——蔗糖合酶活性的调节作用。结果表明，灌浆前期强势粒中生长素、玉米素核苷和脱落酸（ABA）含量明显高于弱势粒，并与籽粒最大灌浆速率、平均灌浆速率、籽粒充实率和粒重均呈显著或极显著正相关。强势粒中蔗糖合酶活性明显高于弱势粒，且达到峰值的时间也早于弱势粒。籽粒灌浆速率、籽粒充实率、粒重和淀粉积累速率与蔗糖合酶活性呈显著正相关。花后8 d对稻株喷施低浓度ABA显著提高了籽粒中ABA含量和蔗糖合酶活性，增加了粒重和籽粒充实率。喷施ABA合成抑制剂氟草酮作用则相反。上述结果说明，植物激素尤其是ABA对水稻籽粒灌浆有重要的促进作用，其作用在很大程度上是通过调节籽粒蔗糖合酶活性实现的。

关键词: 脱落酸, 籽粒充实, 亚种间杂交稻, 蔗糖合酶

CLC Number:

Q945.6

TANG Tang,XIE Hong,LU Bing,LIANG Jian-sheng . Effects of Plant Hormones in Regulating Sucrose Synthase Activity and Grain Filling of Rice[J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2011.02.010 .

唐瑭,谢红,吕冰, 梁建生,. 植物激素对杂交稻籽粒灌浆及蔗糖合酶活性的影响[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2011.02.010 .

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