Abstract: A strain of yeast-like-symbiotes(YLS) was isolated from the eggs of brown planthopper(Nilaparvata lugens St°al,BPH),and named as strain 34.The 26S rDNA sequence of strain 34 was highly homologous with that of the YLS directly isolated from BPH,and the strains both belonged to the Yarrowia lipolytica of subphylum Ascomycotina class Pyrenomycetes.The results showed that the strain 34 had the genetic relationship with the yeast-like-symbiotes of BPH.Moreover,it was concluded that the YLS of BPH could be cultured in vitro

Key words: brown planthopper, culture in vitro, yeast-like symbiotes, Yarrowia lipolyica, strain isolation, molecular identification

摘要： 运用卵块离体培养法，从褐飞虱（Nilaparvata lugens St°〖〗al）卵块中分离筛选到1株类酵母菌菌株，命名为34号菌株。比较34号菌株与直接从褐飞虱虫体内分离的类酵母共生菌(yeastlikesymbiotes, YLS) 26S rDNA序列同源性，并将结果在GenBank 核酸序列数据库中进行同源序列搜索，结果显示两种菌株都与子囊菌亚门（Ascomycotina）核菌纲(Pyrenomycetes)的解脂假丝酵母（Yarrowia lipolytica）显示出较近的亲缘关系，初步表明分离所得34号菌株可能为褐飞虱内共生菌的一种，同时也说明褐飞虱体内共生菌可通过离体培养的方式获得。

关键词: 褐飞虱, 离体培养, 类酵母共生菌, 解脂假丝酵母, 菌株分离, 分子鉴定