Chinese Journal OF Rice Science

Chinese Journal of Rice Science

• 研究报告 •     Next Articles

RFLP Analysis of Euploid Plants from Crosses Between Oryza sativa and Oryza eichingeri

Liu Guoqing; Yan Huihuang; Luo Yaowu; Min Shaokai; Zhu Lihuang   

  • Received:1900-01-01 Revised:1900-01-01 Online:1999-07-10 Published:1999-07-10

栽培稻与紧穗野生稻间整倍体后代的RFLP分析

刘国庆 1;颜辉煌 1，2 罗耀武 3;闵绍楷 2;朱立煌 1,*   

  1. 1 中国科学院遗传研究所，北京 100101； 2 中国水稻研究所，浙江 杭州 310006；3 河北农业大学，河北 保定 071001；*通信联系人，E-mail:lhzhu@ss10.igtp.ac.cn

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Abstract: Sixty seven euploid plants (2n = 24 ,CC) derived from intergenomic crosses between Oryza sativa cv. 02428 (2n =24, AA) and two accessions Oryza eichingeri (2n= 24, CC) were analyzed by using 164 RFLP markers, and 6o plants wereidentified to be introgression lines, each carrying 1 - 6 chromosome segments from O. eichingeri. Most introgression linescontained similar O. eichingeri segments as defined by common RFLP marker(s). The incorporated O. eichingeri segments were small in size and mainly distributed away from centromeres. In addition, the Possible causes for the occurrence of novel bands in euploid plants were discussed.

Key words: Oryza sativa, Oryza eichingeri, euploid plants, restriction fragment length polymorphism (RFLP)

摘要： 用164个RFLP探针对67个栽培稻02428(2n=24，AA)与两份紧穗野生稻材料(2n=24,CC)间的整倍体(2n=24)后代进行分析，证实其中60个为易位系。各易位系分别带有1～6个紧穗野生稻染色体片段，很多易位系具有相同的易位发生区域。易位的紧穗野生稻片段都很小，主要分布在远离着丝点的区域。还就整倍体后代中出现新杂交带型的可能原因进行了讨论。

关键词: 栽培稻, 紧穗野生稻, 整倍体后代, 限制性片段长度多态性