Establishment of Fingerprint Map of Ning 2A with Simple Sequence Repeat Markers

Chinese Journal of Rice Science

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Establishment of Fingerprint Map of Ning 2A with Simple Sequence Repeat Markers

YU Yong-hong; LI Yun-hai; MA Rong-rong; WANG Xiao-yan; HU Guo-cheng; SI Hua-min; FU Ya-ping; SUN Zong-xiu

Received:1900-01-01 Revised:1900-01-01 Online:2001-07-10 Published:2001-07-10

用微卫星DNA标记建立宁2A的指纹图谱

于永红1; 李云海1; 马荣荣2; 王晓燕3; 胡国成1; 斯华敏1; 傅亚萍1; 孙宗修1

1中国水稻研究所，农业部水稻生物学重点实验室，浙江杭州 310006; 2 浙江省宁波市农业科学研究所，浙江宁波 315040； 3 浙江省宁波市种子公司，浙江宁波 315010

Abstract

Abstract: DNA fingerprint map of hybrid rice Ning 2A was established by using selected four SSR primer pairs. The result showed that the first band of RM206 and RM224 of SSR primer pairs is existed specially in Ning 2A. By analyzing genetic relationship between Ning 2A and other parents of hybrid rice, it was found that Ning 2A possessed genetic composition of indica, which explained the reason that Ning 2A have stronger affinity with indica at molecular levels.

Key words: hybrid rice, simple sequence repeats, fingerprint map

摘要： 应用筛选出的4对SSR引物，建立了杂交水稻不育系宁2A 以及宁2B的DNA指纹图谱，结果表明，宁2A/B 特异性的 SSR指纹是引物RM206的谱带1和引物RM224的谱带1。通过与其他广泛应用的杂交水稻亲本材料的遗传关系分析，发现宁2A/B具有籼稻的遗传成分，从而在分子水平上解释了该材料与籼稻亲和性较高的原因。

关键词: 杂交水稻, 简单序列重复, 指纹图谱

YU Yong-hong,LI Yun-hai,MA Rong-rong,WANG Xiao-yan,HU Guo-cheng,SI Hua-min,FU Ya-ping,SUN Zong-xiu. Establishment of Fingerprint Map of Ning 2A with Simple Sequence Repeat Markers[J]. Chinese Journal of Rice Science.

于永红, 李云海, 马荣荣, 王晓燕, 胡国成, 斯华敏, 傅亚萍, 孙宗修. 用微卫星DNA标记建立宁2A的指纹图谱[J]. 中国水稻科学.

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Establishment of Fingerprint Map of Ning 2A with Simple Sequence Repeat Markers

用微卫星DNA标记建立宁2A的指纹图谱

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