Abstract: Seeds of CMS line Liuqianxin A with BT cytoplasm, maintainer line Liuqianxin B, restorer lines Liuqianxin R, 77302-1, F₁ and F₂ of Liuqianxin A/77302-1 in japonica rice were analyzed for endosperm storage proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results showed that in glutelin α-3 area, restorer lines had two bands α-3a and α-3b while Liuqianxin A and Liuqianxin B had only one band α-3. In F₂ generation, seeds with α-3 and seeds with α-3a plus α-3b segregated into 1∶1 ratio. Moving rate of glutelin α-4 band in restorer lines was faster than that in Liu qianxin A. The faster α-4 was designated as α-4f. Seeds with α-4 and seeds with α-4 plus α-4f also segregated into 1∶1 ratio in F₂, which are consistent with the segregation of restorer gene of gametophytic type. Pedigree analysis indicates that the bands α-3a and α-3b in Liuqianxin R derived from IR8. Prolamin in Liuqianxin A is easier to extract than that in Liuqianxin B.

Key words: japonica rice, restorer line, cytoplasm male sterile line, protein marker, hybrid rice, glutelin, prolamin

摘要： 用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）方法，对粳稻BT 细胞质雄性不育系六千辛A，保持系六千辛B，恢复系六千辛R、77302-1，以及杂交组合六千辛A/77302-1的F1和F2 种子的胚乳贮藏蛋白进行了分析。结果表明，在谷蛋白α3区域，恢复系有两条带α3a 和α3b,而六千辛A和六千辛B只有一条带α3。 F2代具有α3的种子和具有α3a 加α3b的种子1∶1分离。谷蛋白α4带的移动速率，恢复系比六千辛A快。把较快的α4带记为α4f。 F2代具有α4的种子和具有α4 加α4f的种子也是1∶1分离，与配子体不育类型的F2代花粉育性恢复基因分离比一致。系谱分析表明六千辛R中α3a 和α3b来源于IR8。六千辛A比六千辛B容易提取醇溶蛋白。

关键词: 粳稻, 恢复系, 细胞质雄性不育系, 蛋白质标记, 杂交水稻, 谷蛋白, 醇溶蛋白