中国水稻科学
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  2001, Vol. 15 Issue (4): 291-297     DOI:
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Factors Affecting on Conidium Germination and Its Appressorium Formation of Magnaporthe grisea
LIN Fu-cheng; LI De-bao
(Biotechnology Institute; Zhejiang University; Hangzhou 310029; China)
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Abstract Conidium germination and appressorium formation rate under eight substrates, different agar surface with various cAMP and IBMX concentration gradients, different nutrient resource, pH value of conidial suspension, and cultural filter of antigonistic bacteria were observed.Conidium density of its suspension and its pH value did not affect the appressorium formation of Magnaporthe grisea 91-11B1, high density ( conidia >10 5/mL) and unsuitable pH value (>10 or <4) would significantly reduce the germination of conidia.The hydrophobic substratum surfaces could enhance the appressorium formation.The starvation of nutrition and the surfaces of onion epidermis could stimulate the appressorium morphogenesis, and rich nutrition could inhibit it. Compared with ion free pure water,tap water could facilitate the appressorium formation. 2.5 mmol/L cAMP and 8.0 mmol/L IBMX, the mostly commonly used phosphodiesterase inhibitor, significantly promote appressorium formation. Conidia which collected by vibrating method were more effective to differentiate into appressorium than those by washing method. Among 13 strains of antagonistic bacteria, three strains, such as Xh216, Xh240 and Xh220 strongly inhibited the germination and appressorium formation.
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LIN Fu-cheng
LI De-bao
Key wordsMagnaporthe grisea   appressorium   environmental factor   germination   conidium     
Received: 1900-01-01;
Cite this article:   
LIN Fu-cheng,LI De-bao. Factors Affecting on Conidium Germination and Its Appressorium Formation of Magnaporthe grisea[J]. , 2001, 15(4): 291-297 .
 
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