Abstract: Conidium germination and appressorium formation rate under eight substrates, different agar surface with various cAMP and IBMX concentration gradients, different nutrient resource, pH value of conidial suspension, and cultural filter of antigonistic bacteria were observed.Conidium density of its suspension and its pH value did not affect the appressorium formation of Magnaporthe grisea 91-11B1, high density ( conidia >10 5/mL) and unsuitable pH value (>10 or <4) would significantly reduce the germination of conidia.The hydrophobic substratum surfaces could enhance the appressorium formation.The starvation of nutrition and the surfaces of onion epidermis could stimulate the appressorium morphogenesis, and rich nutrition could inhibit it. Compared with ion free pure water,tap water could facilitate the appressorium formation. 2.5 mmol/L cAMP and 8.0 mmol/L IBMX, the mostly commonly used phosphodiesterase inhibitor, significantly promote appressorium formation. Conidia which collected by vibrating method were more effective to differentiate into appressorium than those by washing method. Among 13 strains of antagonistic bacteria, three strains, such as Xh216, Xh240 and Xh220 strongly inhibited the germination and appressorium formation.

Key words: Magnaporthe grisea, appressorium, environmental factor, germination, conidium

摘要： 在8种培养基膜，多种cAMP、IBMX浓度梯度的琼脂表面膜，不同营养液及pH值条件下，研究了稻瘟病分子孢子发芽和附着胞形成的状况，并对稻瘟病拮抗细菌培养液的作用进行了研究。稻瘟病菌(Magnaporthe grisea) 91-11B1的分生孢子悬浮液浓度在10000~1000个/mL 和pH值在7.0为最适孢子萌发条件，但不影响附着胞的形成。疏水基物表面、营养饥饿或洋葱表皮细胞表面可以刺激附着胞形成。2.5 mmol/L cAMP和8.0 mmol/L磷酸二酯酶抑制剂IBMX显著地促进附着胞的形成。与去离子水和水洗法相比，自来水和振落法获得的分生孢子更有利于附着胞的形成。筛选到3株对孢子发芽及附着胞形成有强烈抑制作用的拮抗细菌

关键词: 稻瘟病菌, 附着胞, 环境因子, 发芽, 分生孢子