<FONT face="Verdana">水稻籽粒蛋白质含量选择对杂交后代蛋白质含量及氮代谢关键酶活性的影响</FONT>

黄 星,李晓光,刘洪亮,徐美兰, 张丰转,张忠臣,金正勋

doi:10.3969/j.issn.1001-7216.2009.06.16

中国水稻科学 >

2009 , Vol. 23 >Issue 6: 657 - 660

DOI: https://doi.org/10.3969/j.issn.1001-7216.2009.06.16

研究简报

水稻籽粒蛋白质含量选择对杂交后代蛋白质含量及氮代谢关键酶活性的影响

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东北农业大学农学院，黑龙江哈尔滨 150030； *通讯联系人， E-mail: zxjin326@hotmail.com

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2009-11-10

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Effects of Grain Protein Content Selection on Protein Content and Key Enzymes Activities for Nitrogen Metabolism in Rice Hybrid Progenies

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Agricultural College, Northeast Agricultural University，Harbin 150030， China; *Corresponding author, E-mail: zxjin326@hotmail.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2009-11-10

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摘要

以籽粒蛋白质含量有显著差异的杂交后代及亲本为材料，分析了灌浆成熟过程中叶片蛋白水解酶和籽粒谷氨酰胺合成酶活性以及籽粒可溶性蛋白质含量的变化动态。结果表明，在籽粒蛋白质含量相近的亲本衍生的杂交后代中，通过籽粒蛋白质含量的连续定向选择不仅可以获得籽粒蛋白质含量和叶片蛋白水解酶活性及籽粒谷氨酰胺合成酶活性明显变高或变低的杂种后代，而且可获得蛋白质含量和酶活性超亲的后代；稻米蛋白质含量与灌浆过程中的叶片蛋白水解酶活性呈正相关，籽粒可溶性蛋白质含量与籽粒谷氨酰胺合成酶活性呈显著负相关。

关键词： 水稻; 杂交后代; 蛋白质含量; 氮代谢; 酶活性

本文引用格式

黄星,李晓光,刘洪亮,徐美兰, 张丰转,张忠臣,金正勋 . 水稻籽粒蛋白质含量选择对杂交后代蛋白质含量及氮代谢关键酶活性的影响[J]. 中国水稻科学, 2009 , 23(6) : 657 -660 . DOI: 10.3969/j.issn.1001-7216.2009.06.16

Abstract

The japonica parents (Tong 769 and Xixuan 1) and their hybrid progenies, significantly differed in protein content in grains, were investigated to reveal the activities of protease in leaves and glutamine synthetase in grains and the dynamic alteration of soluble protein content in grains during grain filling. The results showed that the protein content in rice of the parents was near to each other, however, hybrid progenies with different contents of protein in grains and higher or lower activities of protease in leaves and glutamine synthetase in grains were acquired by consecutively directed selection of the grain protein content in their hybrid progenies. Moreover, the enzyme activity and protein content in grains exceled their parents during grain filling. The content of protein in rice and the activitiy of protease in leaves were positively related, and the content of soluble protein and activity of glutamine synthetase in grains were negatively related to some extent.

Key words： rice; hybrid progeny; protein content; nitrogen metabolism

参考文献

[1]Matsue Y. Differences in amylose content, amylographic characteristics and storage proteins of grain on primary and secondary rachis branches in rice. Jpn J Crop Sci, 1995, 64(3): 601-606.

[2]钱春荣，冯延江，杨静，等. 水稻籽粒蛋白质含量选择对杂种早代蒸煮食味品质的影响. 中国水稻科学， 2007，21（3） 323-326.

[3]Lea D J, Robinson S A, Stewart G R. The enzymology and metabolism of glutamate, and asporagine//Miflin B J, Lea P J. The Biochemistry of Plants. Vol. 16. New York: Academic Press, 1990, 16:121-159.

[4]布坎南 B B, 格鲁依森姆 W，琼斯 R L. 植物生物化学与分子生物学. 瞿礼嘉，顾红雅，白书农，等译. 北京：科学出版社， 2002.

[5]沈成国. 植物衰老生理与分子生物学. 北京：中国农业出版社， 2001.

[6] 娄成后，王学臣. 作物产量形成的生理学基础. 北京：中国农业出版社， 2001.

[7]刘海英, 杨静, 钱春荣, 等. 6-苄氨基嘌呤对水稻灌浆成熟期籽粒氮代谢及蒸煮食味品质的影响. 中国水稻科学, 2006, 20（6）：667-669.

[8]罗兰芳, 郑圣先, 廖育林, 等. 控释氮肥对杂交水稻糙米蛋白质品质和氮代谢关键酶活性的影响. 中国水稻科学, 2007, 21（4）：403-410.

[9]王亮, 朱建国, 朱春梧, 等. 高浓度CO2条件下水稻叶片氮含量下降与氮代谢关键酶活性的关系. 中国水稻科学, 2008, 22（5）：499-506.

[10]Xie Z J, Jiang D, Cao W X, et al. Effects of post anthesis soil water status on the key regulatory enzymes of starch and protein accumulation in wheat grains. J Plant Physiol Mol Biol, 2003, 29(4): 309-316.

[11]朱红梅, 荣湘民, 刘强, 等. 不同基因型水稻籽粒蛋白质含量差异的研究. 湖南农业大学学报, 2001, 27（1）： 13-16.

[12] 唐湘如, 官春云, 余铁桥. 不同基因型水稻产量和品质的物质代谢研究. 湖南农业大学学报, 1999, 25（4）： 279-282.

[13] 唐湘如, 余铁桥. 密度对饲用杂交稻几种酶活性的影响及其与产量和蛋白质含量的关系. 杂交水稻, 2001, 16（6）： 47-49.

[14]金正勋, 钱春荣, 杨静, 等. 水稻灌浆成熟期籽粒谷氨酰胺合成酶活性变化及其与稻米品质关系的初步研究. 中国水稻科学, 2007, 21（1）： 103-106.

[15] 张青, 叶茂炳, 徐朗莱, 等.小麦老化叶片蛋白质水解酶的某些生化特征的研究. 植物生理学通讯, 1997, 33(5): 336-338.

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