OsERF93参与调控水稻纹枯病抗性的研究
收稿日期: 2025-02-11
修回日期: 2025-05-23
网络出版日期: 2026-01-21
基金资助
国家自然科学基金资助项目(32272110);国家自然科学基金资助项目(32200430);生物育种钟山实验室项目(ZSBBL-KY2023-06-3);扬州市现代农业项目(YZ2024045)
Function of OsERF93 in Regulating Resistance to Sheath Blight in Rice
Received date: 2025-02-11
Revised date: 2025-05-23
Online published: 2026-01-21
【目的】本研究旨在解析ERF类转录因子OsERF93在水稻抗纹枯病中的功能与机制，为抗病育种提供基因资源。【方法】利用RT-qPCR、水稻原生质体转录激活等方法，分析了OsERF93的表达模式、转录调控功能和亚细胞定位位置；通过构建转基因过表达和敲除株系，研究了OsERF93调控纹枯病抗性的功能，进而比较分析了水杨酸、茉莉酸和乙烯信号相关基因在转基因系接种前后的表达差异，以及超表达转基因系与对照间的农艺性状差异。【结果】OsERF93在水稻叶片和叶鞘组织中高表达，受纹枯病菌侵染诱导表达；其编码蛋白定位于细胞核和细胞膜中，具有转录激活活性；OsERF93过表达显著增强了水稻纹枯病抗性，而敲除则显著减弱抗病性；茉莉酸合成基因OsAOS2以及部分PR基因在OsERF93过表达系和敲除系中分别明显上调和下调表达；OsERF93过表达系的株高和千粒重略低于野生型对照，但每穗粒数显著高于对照，其他性状如分蘖数、一次枝梗数和穗长与对照间无显著差异。【结论】OsERF93通过激活茉莉酸信号途径正调控水稻的纹枯病抗性，为水稻抗病育种提供了一个新的基因资源。
岳轩宇, 谢文亚, 冯志明, 陈宗祥, 胡珂鸣, 左示敏 . OsERF93参与调控水稻纹枯病抗性的研究[J]. 中国水稻科学, 2026 , 40(1) : 37 -50 . DOI: 10.16819/j.1001-7216.2026.250202
【Objective】This study aims to elucidate the function and mechanism of the ERF transcription factor OsERF93 in rice sheath blight resistance, providing genetic resources for disease-resistant breeding.【Method】The expression pattern, transcriptional regulatory function, and subcellular localization of OsERF93 were analyzed using RT-qPCR and transcriptional activation assays in rice protoplasts. Transgenic overexpression and knockout lines were constructed to investigate the role of OsERF93 in sheath blight resistance. Expression differences of salicylic acid (SA), jasmonic acid (JA), and ethylene (ET)-related signaling genes were compared before and after inoculation in transgenic lines. Additionally, agronomic traits of overexpression lines and controls were evaluated.【Result】OsERF93 was highly expressed in rice leaves and leaf sheaths and induced by sheath blight fungus infection. The encoded protein localized to the nucleus and cell membrane, exhibiting transcriptional activation activity. Overexpression of OsERF93 significantly enhanced sheath blight resistance, while knockout lines showed reduced resistance. JA biosynthesis gene OsAOS2 and certain PR genes were upregulated in overexpression lines and downregulated in knockout lines. Overexpression lines exhibited slightly reduced plant height and thousand-grain weight compared to wild-type controls but had significantly higher grain number per panicle. No significant differences were observed in tiller number, primary branch number, and panicle length between overexpression lines and controls.【Conclusion】The results indicate that OsERF93 positively regulates rice sheath blight resistance by activating the jasmonic acid signaling pathway, offering a novel genetic resource for rice disease-resistant breeding.
Key words： rice; sheath blight; OsERF93; jasmonic acid; agronomic trait
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