水稻OsDR8基因的稻瘟病抗性评价及优异单倍型鉴定
收稿日期: 2024-05-16
修回日期: 2024-07-19
网络出版日期: 2025-05-21
基金资助
国家重点研发计划资助项目(2022YFE0139400);深圳市科技计划资助项目(GJHZ20190821163601707);福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021002)
Evaluation of Blast Resistance and Identification of Superior Haplotype of OsDR8 in Rice
Received date: 2024-05-16
Revised date: 2024-07-19
Online published: 2025-05-21
【目的】水稻(Oryza sativa L.)是全球重要的主粮作物，其生产安全受到稻瘟病的严重威胁。抗稻瘟病材料的鉴定及相关抗稻瘟病基因的解析，有助于揭示稻瘟病抗性机制。本研究旨在系统创制和鉴定水稻抗稻瘟病基因OsDR8的遗传材料，并对其进行单倍型和进化分析，以期筛选优异单倍型，探究其亚群分化，为OsDR8基因的抗病育种应用提供理论依据。【方法】创制了OsDR8基因的过表达株系和敲除株系，并用RO1-1和RB22稻瘟菌对过表达株系、纯合敲除株系和对应野生型材料进行稻瘟病抗性鉴定。同时，基于水稻超级泛基因组变异图谱，进行OsDR8基因的单倍型分析和进化分析。【结果】对这些材料进行症状观察和稻瘟病抗性鉴定，发现相比野生型株系，敲除突变体株系病斑显著增大，发病叶面积占比也显著上升；而过表达株系病斑显著减小，发病叶面积占比也显著降低。结合单倍型及进化分析，说明携带优异单倍型Hap2的材料抗病性较强，籼稻亚群中95.52%材料为Hap2单倍型。【结论】OsDR8基因正调控水稻稻瘟病抗性，优异单倍型Hap2可能与籼稻稻瘟病抗性较强有关。
张彬涛, 刘聪聪, 郭明亮, 杨绍华, 吴世强, 郭龙彪, 朱义旺 . 水稻OsDR8基因的稻瘟病抗性评价及优异单倍型鉴定[J]. 中国水稻科学, 2025 , 39(3) : 343 -351 . DOI: 10.16819/j.1001-7216.2025.240509
【Objective】Rice (Oryza sativa L.) is a crucial staple crop globally. Its production is severely threatened by rice blast disease. Identifying resistance materials and analyzing resistance genes are essential for understanding the mechanisms behind rice blast resistance. This study aims to systematically generate and identify genetic materials of the rice blast resistance gene OsDR8, conduct haplotype and evolutionary analyses to select superior haplotypes, and investigate their subpopulation differentiation. These efforts will lay a theoretical basis for the application of OsDR8 in rice blast resistance breeding programs.【Method】We developed transgenic lines overexpressing the OsDR8 gene and its knockout mutants. We conducted rice blast resistance assays on the overexpression lines, homozygous knockout lines, and corresponding wild-type materials using Magnaporthe oryzae isolates RO1-1 and RB22. Meanwhile, haplotype and evolutionary analyses of the OsDR8 gene were conducted based on the rice super pan-genome variation map.【Result】Disease symptom observation and disease resistance evaluation indicated that knockout mutants had significantly larger lesion sizes and a higher proportion of diseased leaf area compared to the wild-type. In contrast, the overexpression lines showed reduced lesion sizes and a lower proportion of diseased leaf area. Combined with haplotype and evolutionary analyses, the results indicated that materials of the superior haplotype Hap2 show stronger disease resistance, with 95.52% of the indica rice subpopulation being the Hap2 haplotype.【Conclusion】The OsDR8 gene positively regulates resistance to rice blast, and that the superior haplotype Hap2 may be associated with stronger resistance in indica rice.
Key words： rice; rice blast; superior haplotype; breeding application
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