水稻抽穗期调控基因Hd6功能标记的开发及应用
收稿日期: 2023-10-24
修回日期: 2023-11-30
网络出版日期: 2025-01-14
基金资助
江苏省种业振兴“揭榜挂帅”项目(JBGS〔2021〕039);江苏省重点研发计划（现代农业）资助项目(BE2021374);江苏省重点研发计划（现代农业）资助项目(BE2022382);江苏省重点研发计划（现代农业）资助项目(1002);生物育种钟山实验室项目(BM2022008-03)
Development and Application of a Functional Marker for Heading Date Gene Hd6 in Rice
Received date: 2023-10-24
Revised date: 2023-11-30
Online published: 2025-01-14
【目的】探究Hd6等位基因型在江苏粳稻和籼稻中的分布，提高Hd6等位基因型在水稻抽穗期遗传改良中的应用价值和选择效率。【方法】根据籼稻品种Kasalath中有功能的等位基因Hd6与粳稻品种日本晴中无功能的等位基因hd6在功能区域存在的单核苷酸差异，设计和筛选出Hd6基因功能标记K-Hd6-21F/Hd6-1R和N-Hd6-22F/Hd6-1R，并测序分析验证；利用K-Hd6-21F/Hd6-1R和N-Hd6-22F/Hd6-1R对江苏省不同生态类型的粳稻品种和不同来源的籼稻品种的Hd6基因型进行检测。【结果】该功能标记可准确鉴定出Hd6的不同基因型；48份中熟中粳水稻品种中，携带Hd6的水稻品种占42%；30份迟熟中粳水稻品种中携带Hd6的占50%；48份早熟晚粳水稻品种中，携带Hd6的占77.5%。Hd6的2种等位基因型在江苏省3个不同生态区域粳稻品种中均有分布，但有功能的Hd6等位基因型在3个区域粳稻品种中的分布随着抽穗期变长，基因型频率增加；而Hd6基因在籼稻中表现出高度保守性，检测的62个籼稻品种均携带有功能的Hd6等位基因型。【结论】本研究为Hd6基因的育种利用和分子标记辅助选择奠定了基础，也为籼稻早熟品种的选育提供新依据和有效途径。
关键词： 水稻; 抽穗期; Hd6; 功能标记; 等位基因特异性PCR
陈智慧, 陶亚军, 范方军, 许扬, 王芳权, 李文奇, 古丽娜尔·巴合提别克, 蒋彦婕, 朱建平, 李霞, 杨杰 . 水稻抽穗期调控基因Hd6功能标记的开发及应用[J]. 中国水稻科学, 2025 , 39(1) : 47 -54 . DOI: 10.16819/j.1001-7216.2025.231009
【Objective】This study aims to explore the distribution of Hd6 alleles in japonica rice and indica rice from Jiangsu Province, and to enhance the application value and selection efficiency of Hd6 alleles in the genetic improvement of rice heading date. 【Method】We designed and screened various types of gene functional markers based on the single nucleotide polymorphisms in the functional regions of the Hd6 alleles in Kasalath and hd6 in Nipponbare. Through PCR amplification and sequencing, we selected the gene markers K-Hd6-21F/Hd6-1R and N-Hd6-22F/Hd6-1R to genotype japonica rice varieties from different ecological types in Jiangsu Province and indica rice varieties from various sources.【Result】We found that K-Hd6-21F/Hd6-1R and N-Hd6-22F/Hd6-1R could effectively distinguish different genotypes at the Hd6 locus. The results indicated that 42% of the 48 medium-maturing japonica rice varieties carried the Hd6 allele, 50% of the 30 late-maturing japonica rice varieties carried Hd6, and 77.5% of the 48 early-maturing japonica rice varieties carried Hd6. The two alleles of Hd6 were distributed among varieties from three different ecological regions in Jiangsu Province, and the genotype frequency of functional Hd6 alleles increased with the heading date. The Hd6 gene is highly conserved in indica rice and was tested in all 62 indica varieties.【Conclusion】This study lays a foundation for the utilization of the Hd6 gene and molecular marker-assisted selection in rice breeding, providing a new basis and effective approach for the breeding of early-maturing varieties in indica rice.
Key words： rice; heading date; Hd6; functional marker; allele-specific PCR
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