【目的】为了探明淹水逆境下水稻种子向胚芽供应葡萄糖能力的品种间差异及其与胚芽存活的关系，从而解析水稻芽苗期耐淹机理。【方法】以4个淹水耐性不同的品种为试验材料，设置长期淹水和湿润播种(对照)两个处理，分析种子和胚芽组织的非结构性碳水化合物含量，种子α-淀粉酶活性、胚芽抗氧化酶活性随时间的动态变化，及其与胚芽存活率的相关性。【结果】淹水胁迫下，淹水敏感材料中嘉8号(S1)和中嘉早17(S2)胚芽的葡萄糖含量、SOD、POD酶比蛋白活性在淹水72h后显著下降，表型上表现为胚芽生长停滞并出现死亡症状；而耐淹材料日本晴(T1)、耘两优玖48(T2)的胚芽中葡萄糖含量、SOD、POD的酶比蛋白活性在淹水96 h内保持稳定，胚芽持续伸长。对种子α-淀粉酶活性的分析表明，虽然短期淹水(0－48 h)能够增强所有4个材料的种子α-淀粉酶活性，但随淹水时间的延长(72－96h)，仅耐淹的T1、T2保持α-淀粉酶高活性，其种子内的淀粉含量持续下降而葡萄糖含量不断增加，胚芽葡萄糖含量相对稳定；而淹水敏感材料S1、S2种子内的α-淀粉酶活性则分别在淹水72 h和48 h后下降，种子内淀粉含量相对稳定，但种子和胚芽的葡萄糖含量大幅度下降。相关分析表明，胚芽的存活率与胚芽和种子的葡萄糖含量、胚芽的过氧化物酶(POD)活性极显著正相关，但与种子淀粉含量极显著负相关。【结论】淹水胁迫下胚芽组织消耗大量的葡萄糖维持其生理代谢平衡，种子向胚芽供应葡萄糖的能力直接关系到其存活，种子α-淀粉酶活性变化对胚芽葡萄糖的供应有决定性作用，是种子在淹水条件下存活出苗的关键。

【Objective】To investigate varietal differences in germ glucose supply under flooded conditions and its relationship with rice germ survival.【Method】Germinated seeds of four rice varieties with different flooding tolerance were used as material and subjected to flooding and wet (control) conditions. Dynamic changes of non-structural carbohydrate contents in seed and germ tissue, seed α-amylase activities, and germ antioxidant enzyme activities over time were analyzed, along with their correlation with seed survival rates.【Result】Under flooding stress, the glucose content and the activities of SOD and POD in the germs of the flooding-sensitive rice materials, Zhongjiazao 8(S1) and Zhongjiazao 17(S2), decreased significantly after 72 hours of flooding, leading to stagnation of germ growth and death. However, the embryo glucose content and the activities of SOD and POD remained stable in the germs of the flooding-tolerant variety Nipponbare(T1) and Yunliangyoujiu 48(T2) after 96 hours of flooding treatment, allowing the germs to continue growing and elongating. Analysis of seed α-amylase activities showed that while short-term flooding (0－48h) enhanced seed α-amylase activities in all four materials, only flooding-tolerant varieties T1 and T2 maintained high α-amylase activity under prolonged flooding (72－96h), leading to a decline in starch content within their seeds and an increase in glucose content. In contrast, the α-amylase activities of flood-sensitive varieties S1 and S2 declined after 48 and 72 hours of flooding treatment, respectively, with relatively stable starch content in the seeds but substantial declines in glucose content of the seeds and the germ. Correlation analysis revealed that germ survival was highly positively correlated with germ and seed glucose content, and POD activity of germ, while highly negatively correlated with seed starch content.【Conclusion】Under flooding stress, the germ tissue consumes a large amount of glucose to maintain its physiological metabolic balance, and the ability of seeds to supply glucose to the embryo is directly related to its survival. Changes in seed α-amylase activity play a pivotal role in the glucose supply to the germ, which is crucial for the survival and emergence of the seed under flooding stress.