氮素穗肥对杂交籼稻籽粒灌浆影响及其与淀粉合成关键酶活性间关系
收稿日期: 2023-04-03
修回日期: 2023-11-09
网络出版日期: 2024-01-16
基金资助
四川省重大科技专项(2022ZDZX0012);四川省重点实验室科学基金资助项目(2022JDS0018)
Effect of Nitrogen Fertilizer Topdressing for Panicle Differentiation on Grain Filling of Hybrid indica Rice and Its Relationship with the Activities of Key Enzymes for Starch Synthesis
Received date: 2023-04-03
Revised date: 2023-11-09
Online published: 2024-01-16
【目的】为明确氮素穗肥对杂交稻籽粒灌浆和淀粉合成关键酶活性的影响。【方法】选用两个直链淀粉含量差异较大的杂交稻品种锦优1319(高直链淀粉)和蓉优702(低直链淀粉)，在不同的氮素穗肥处理下(设0、60、90和120 kg/hm2 4个纯氮水平，分别用CK、N1、N2、N3表示)研究氮素穗肥水平对籽粒灌浆和淀粉合成关键酶活性的影响。【结果】不同穗肥处理下杂交稻籽粒灌浆特性差异显著，且不同类型品种表现各异，其中氮素穗肥的施用显著降低了稻米的直链淀粉含量。锦优1319的Gmax和Gmean随氮素穗肥水平的变化均表现为N2>CK>N1>N3，而蓉优702则表现为N3>N2>CK>N1；施用氮素穗肥显著增加了花后5~25 d籽粒腺苷二磷酸焦磷酸化酶(ADPG)和淀粉分支酶(DBE)的活性，降低了花后5~15 d籽粒GBSS活性，降低了花后5~25 d籽粒淀粉合成酶(SSS)和SBE活性。【结论】高直链淀粉品种锦优1319籽粒灌浆的最佳氮素穗肥施用量低于低直链淀粉品种蓉优702：其中锦优1319的最佳氮素穗肥施用量为90 kg/hm2，蓉优702为120 kg/hm2。施用氮素穗肥显著降低了GBSS、SSS的活性，加之SBE活性下降，DBE活性提高，最终降低稻米直链淀粉含量。本研究条件下，籽粒ADPG活性未对稻米直链淀粉含量产生显著影响。
吴子牛, 何丽梅, 熊莹, 陈凯瑞, 杨志远, 孙永健, 吕旭, 马均 . 氮素穗肥对杂交籼稻籽粒灌浆影响及其与淀粉合成关键酶活性间关系[J]. 中国水稻科学, 2024 , 38(1) : 48 -56 . DOI: 10.16819/j.1001-7216.2024.230401
【Objective】 To elucidate the impact of nitrogen panicle fertilizer on the key enzymes involved in grain filling and starch synthesis in hybrid rice. 【Methods】 Two hybrid rice varieties, Jinyou 1319 (high amylose) and Rongyou 702 (low amylose), characterized by substantial differences in amylose content, were chosen for a study examining the effects of the levels of nitrogen fertilizer topdressing for panicle differentiation on the activities of key enzymes in grain filling and starch synthesis. Various nitrogen topdressing treatments were applied, representing four pure nitrogen levels (0, 60, 90, and 120 kg/hm2 denoted as CK, N1, N2, and N3, respectively). 【Results】 Grain filling characteristics of hybrid rice exhibited significant variations under different nitrogen fertilizer topdressing treatments, with diverse performances observed among different varieties. The application of nitrogen fertilizer topdressing notably decreased the amylose content of rice. Both Gmax and Gmean of Jinyou 1319 exhibited a trend of N2>CK>N1>N3 under different nitrogen fertilizer topdressing levels, while those of Rongyou 702 followed a tendency of N3>N2>CK>N1. The nitrogen fertilizer topdressing significantly increased the activities of ADPG and DBE in grains at 5－25 days after anthesis, reduced the activities of GBSS in grains at 5－15 days after anthesis, and decreased the activities of SSS and SBE in grains at 5－25 days after anthesis. 【Conclusion】 The optimal application rate of nitrogen fertilizer topdressing for panicle differentiation was lower for the high-amylose variety Jinyou 1319 compared to the low-amylose variety Rongyou 702: the optimal rate of nitrogen fertilizer topdressing for Jinyou 1319 was 90 kg/hm2, and for Rongyou 702, it was 120 kg/hm2. The nitrogen fertilizer topdressing significantly reduced the activities of GBSS and SSS, accompanied by a decrease in SBE activity and an increase in DBE activity, ultimately leading to a reduction in rice amylose content. Seed ADPG activity did not significantly affect rice amylose content in this study.
| [1] | 杨建昌, 张建华. 促进稻麦同化物转运和籽粒灌浆的途径与机制[J]. 科学通报 2018, 63 (Z2): 2932-2943. |
| [1] | Yang J C, Zhang J H. Approach and mechanism in enhancing the remobilization of assimilates and grain-filling in rice and wheat[J]. Chinese Science Bulletin, 2018, 63(Z2): 2932-2943. (in Chinese with English abstract) |
| [2] | 李强强, 赵玥, 李璠, 朱一鸣, 杨成淦, 曹佳乐, 王志琴, 杨建昌, 顾骏飞. 作物源库关系及其生理调控途径的研究进展[J]. 江苏农业科学 2020, 48(9): 50-56. |
| [2] | Li Q Q, Zhao Y, Li F, Zhu Y M, Yang C G, Cao J L, Wang Z Q, Yang J C, Gu J F. Research progress of source-sink relationship and its underlying regulation mechanisms[J]. Jiangsu Agricultural Sciences, 2020, 48 (9): 50-56. (in Chinese with English abstract) |
| [3] | 张艳霞, 丁艳锋, 王强盛, 李刚华, 李福春, 王绍华. 氮素穗肥对不同品种稻米品质性状的影响[J]. 植物营养与肥料学报, 2007(6): 1080-1085. |
| [3] | Zhang Y X, Ding Y F, Wang Q S, Li G H, Li F C, Wang S H. Effect of panicle nitrogen fertilizer on quality properties of different rice varieties[J]. Journal of Plant Nutrition and Fertilizers, 2007(6): 1080-1085. (in Chinese with English abstract) |
| [4] | 殷春渊, 宁惠峰, 赵全志, 陈静蕊. 氮素穗肥施用量对沿黄稻区稻米品质和食味的影响[J]. 河南农业科学, 2007(5): 18-20+24. |
| [4] | Ying C Y, Ning H F, Zhao Q Z, Chen J R. Effect s of N-fertilizer application amount at the panicle stage on rice quality and food taste along the yellow river valley[J]. Journal of Henan Agricultural Sciences, 2007(5): 18-20+24. (in Chinese with English abstract) |
| [5] | 程方民, 蒋德安, 吴平, 石春海. 早籼稻籽粒灌浆过程中淀粉合成酶的变化及温度效应特征[J]. 作物学报, 2001(2): 201-206. |
| [5] | Chen F M, Jiang D A, Wu P, Shi C H. The dynamic change of starch synthesis enzymes during the grain filling stage and effects of temperature upon it[J]. Acta Agronomica Sinica, 2001(2): 201-206. (in Chinese with English abstract) |
| [6] | 程方民, 钟连进, 孙宗修. 灌浆结实期温度对早籼水稻籽粒淀粉合成代谢的影响[J]. 中国农业科学, 2003, 36(5): 492-501. |
| [6] | Chen F M, Zhong L J, Sun Z X. Effect of temperature at grain-filling stage on starch biosynthetic V metabolism in developing rice grains of early-indica[J]. Scientia Agricultura Sinica, 2003, 36(5): 492-501. (in Chinese with English abstract) |
| [7] | 钟连进, 程方民. 水稻籽粒灌浆过程直链淀粉的积累及其相关酶的品种类型间差异[J]. 作物学报, 2003(3): 452-456. |
| [7] | Zhong L J, Chen F M. Varietal differences in amylose accumulation and activities of major enzymes associated with starch synthesis during grain filling in rice[J]. Acta Agronomica Sinica, 2003(3): 452-456. (in Chinese with English abstract) |
| [8] | Ahmed N, Tetlow I J, Nawaz S, Iqbal A, Mubin M, Nawaz U l, Rehman M S, Butt A, Lightfoot D A, Maekawa M. Effect of high temperature on grain filling period, yield, amylose content and activity of starch biosynthesis enzymes in endosperm of basmati rice[J]. Journal of the Science of Food and Agriculture, 2015, 95 (11): 2237-2243. |
| [9] | Hu J, Zhang S, Yang S, Zhou J, Jiang Z, Qi S, Xu Y. Effects of irrigation and fertilization management on yield and quality of rice and the establishment of a quality evaluation system[J]. Agronomy, 2023, 13 (8), 2034. |
| [10] | 朱立楠, 刘海英, 孙璐璐, 孙涛, 郭雪冬, 朱方旭, 张忠臣, 金正勋. 水稻灌浆过程中胚乳异淀粉酶基因家族表达特性及其与淀粉含量间关系分析[J]. 中国水稻科学, 2015, 29(5): 528-534. |
| [10] | Zhu L N, Liu H Y, Sun L L, Sun T, Guo X D, Zhu F X, Zhang Z C, Jing Z X. Analysis of expression characteristics of isoamylase and the correlation with starch content during grain filling in rice[J]. Chinese Journal of Rice Science, 2015, 29(5): 528-534. (in Chinese with English abstract) |
| [11] | 朱庆森, 曹显祖, 骆亦其. 水稻籽粒灌浆的生长分析[J]. 作物学报, 1988(3): 182-193. |
| [11] | Zhu Q S, Cao X Z, Luo Y Q. Groeth analysis on the process of grain filling in rice[J]. Acta Agronomica Sinica, 1988(3): 182-193. (in Chinese with English abstract) |
| [12] | You C, Chen L, He H, Wu L, Wang S, Ding Y, Ma C. iTRAQ-based proteome profile analysis of superior and inferior spikelets at early grain filling stage in Japonica rice[J]. BMC Plant Biology, 2017, 17(1): 100. |
| [13] | 习敏, 许有尊, 孙雪原, 吴文革, 周永进. 氮素穗肥对水稻垩白籽粒灌浆影响及与加工品质的关系[J]. 中国农业科技导报, 2021, 23(9): 144-151. |
| [13] | Xi M, Xu Y Z, Sun X Y, Wu W G, Zhou Y J. Effects of nitrogen fertilizer topdressing on grain filling and milling quality of the rice with high grain chalkiness[J]. Journal of Agricultural Science and Technology, 2021, 23(9): 144-151. (in Chinese with English abstract) |
| [14] | 马均, 明东风, 马文波, 许凤英. 不同施氮时期对水稻淀粉积累及淀粉合成相关酶类活性变化的研究[J]. 中国农业科学, 2005(2): 290-296. |
| [14] | Ma J, Ming D F, Ma W B, Xu F Y. Changes in starch accumulation and activity of enzymes associated with starch synthesis under different N supplying date[J]. Scientia Agricultura Sinica, 2005(2): 290-296. (in Chinese with English abstract) |
| [15] | 周乾聪, 陈乐, 罗亢, 刘梦洁, 宋永苹, 谢小兵, 曾勇军. 氮素穗肥运筹方式对杂交晚粳稻产量和品质的影响[J]. 作物杂志, 2021(6): 129-133. |
| [15] | Zhou Q C, Cheng L, Luo K, Liu M J, Song Y P, Xie X B, Zeng Y J. Effects of nitrogen panicle fertilizer application on yield and quality of late japonica hybrid rice[J]. Crops, 2021(6): 129-133. (in Chinese with English abstract) |
| [16] | 张庆, 胡雅杰, 郭保卫, 张洪程, 徐玉峰, 徐晓杰, 朱邦辉, 徐洁芬, 钮中一, 凃荣文. 氮素穗肥施用时期对软米粳稻产量和品质的影响[J]. 扬州大学学报: 农业与生命科学版, 2021, 42(5): 72-77. |
| [16] | Zhang Q, Hu Y J, Guo B W, Zhang H C, Xu Y F, Xu X J, Zhu B H, Xu J F, Niu Z Y, Xu R W. Effect of ear nitrogen fertilizer application periods on yield and quality of soft japonica rice[J]. Journal of Yangzhou University: Agricultural and Life Science Edition, 2021, 42(5), 72-77. (in Chinese with English abstract) |
| [17] | Smidansky E D, Meyer F D, Blakeslee B, Weglarz T E, Greene T W, Giroux M J. Expression of a modified ADP-glucose pyrophosphorylase large subunit in wheat seeds stimulates photosynthesis and carbon metabolism[J]. Planta, 2007, 225 (4): 965-976. |
| [18] | Devi T A, Sarla N, Siddiq E A, Sirdeshmukh R. Activity and expression of adenosine diphosphate glucose pyrophosphorylase in developing rice grains: Varietal differences and implications on grain filling[J]. Plant Science, 2010, 178(2): 123-129. |
| [19] | 刘新茹. 肥密对水稻生长发育和碳氮代谢及产量和品质的影响[D]. 哈尔滨: 东北农业大学, 2010. |
| [19] | Liu X R. The effect of the fertilizer and planting density on the activity of key enzyme involved in carbon and nitrogen metabolism, grain yield and quality[D]. Harbin: Northeast Agricultural University, 2010. (in Chinese with English abstract) |
| [20] | 陈云, 刘昆, 张宏路, 李思宇, 张亚军, 韦佳利, 张耗, 顾骏飞, 刘立军, 杨建昌. 机插密度和穗肥减量对优质食味水稻品种籽粒淀粉合成的影响[J]. 作物学报, 2021, 47(8): 1540-1550. |
| [20] | Chen Y, Liu K, Zhang H L, Li S Y, Zhang Y J, Wei J L, Zhang H, Gu J F, Liu L J, Yang J C. Effects of machine transplanting density and panicle nitrogen fertilizer reduction on grains starch synthesis in good taste rice cultivars[J]. Acta Agronomica Sinica, 2021, 47(8): 1540-1550. (in Chinese with English abstract) |
| [21] | 韩展誉, 吴春艳, 许艳秋, 黄福灯, 熊义勤, 管弦悦, 周庐建, 潘刚, 程方民. 不同施氮水平下灌浆期高温对水稻贮藏蛋白积累及其合成代谢影响[J]. 中国农业科学 2021, 54(7): 1439-1454. |
| [21] | Han Z Y, Wu C Y, Xu Y Q, Huang F D, Xio Y Q, Guang X Y, Zhou L J, Pang G, Chen F M. Effects of high-temperature at filling stage on grain storage protein accumulation and Its biosynthesis metabolism for rice plants under different nitrogen application levels[J]. Scientia Agricultura Sinica, 2021, 54(7): 1439-1454. (in Chinese with English abstract) |
| [22] | 段骅, 傅亮, 剧成欣, 刘立军, 杨建昌. 氮素穗肥对高温胁迫下水稻结实和稻米品质的影响[J]. 中国水稻科学, 2013, 27(6): 591-602. |
| [22] | Duan H, Fu L, Ju C X, Liu L J, Yang J C. Effect of application of nitrogen as panicle-promoting fertilizer on seed setting and grain quality of grain quality of rice under high temperature stress[J]. Chinese Journal of Rice Science, 2013, 27(6): 591-602. (in Chinese with English abstract) |
| [23] | 江立庚, 曹卫星, 甘秀芹, 韦善清, 徐建云, 董登峰, 陈念平, 陆福勇, 秦华东. 不同施氮水平对南方早稻氮素吸收利用及其产量和品质的影响[J]. 中国农业科学, 2004(4): 490-496. |
| [23] | Jiang L G, Cao W X, Gan X Q, Wei S Q, Xu J Y, Dong D F, Cheng N P, Lu F Y, Qing H D. Nitrogen uptake and utilization under different nitrogen management and influence on grain yield and quality in rice[J]. Scientia Agricultura Sinica, 2004(4): 490-496. (in Chinese with English abstract) |
| [24] | Zhu D, Zhang H, Guo B, Xu K, Dai Q, Wei H, Gao H, Hu Y, Cui P, Huo Z. Effects of nitrogen level on yield and quality of japonica soft super rice[J]. Journal of Integrative Agriculture, 2017, 16(5): 1018-1027. |
| [25] | 唐健, 唐闯, 郭保卫, 张诚信, 张振振, 王科, 张洪程, 陈恒, 孙明珠. 氮肥施用量对机插优质晚稻产量和稻米品质的影响[J]. 作物学报, 2020, 46(1): 117-130. |
| [25] | Tang J, Tang C, Guo B W, Zhang C X, Zhang Z Z, Wang K, Zhang H C, Cheng H, Sun M Z. Effect of nitrogen application on yield and rice quality of mechanical trans-planting high quality late rice[J]. Acta Agronomica Sinica, 2020, 46(1): 117-130. (in Chinese with English abstract) |
/
| 〈 |
|
〉 |