收稿日期: 2014-05-20
修回日期: 2014-07-15
网络出版日期: 2015-07-10
基金资助
国家自然科学基金资助项目(31301304,31101403);国家科技(转基因)重大专项(2014ZX0800904B);公益性行业科研专项(201203014);广东省自然科学基金资助项目(S2012010010018);国家重点实验室开放课题资助项目(SKL-CUSAb-2013-10);现代农业产业技术体系建设专项(CARS-01-24, 粤财教[2009]356 号)
Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/z-t
Received date: 2014-05-20
Revised date: 2014-07-15
Online published: 2015-07-10
通过对已克隆的抗稻瘟病基因Pi2、Pi9以及Piz-t 进行序列比对,寻找各自特异的核苷酸差异,成功开发了基于PCR技术以及电泳检测技术的 Pi2/Pi9/Piz-t 以及Piz-t 的基因特异性分子标记,能有效地将 Pi2/Pi9/Piz-t 与该位点上的其他抗性等位基因及感病等位基因区分开,这为分子标记辅助育种及抗病基因聚合提供有效的分子标记。用 Pi2/Pi9/Piz-t 基因特异性分子标记对来自全国各稻区的共101份水稻品种和育种亲本进行分子检测,结果发现,除2个品种检测到Piz-t带型之外,大部分水稻品种不携带这3个抗性基因,这为有目的地开展品种的抗性改良提供了重要的参考信息。
华丽霞, 汪文娟, 陈深, 汪聪颖, 曾烈先, 杨健源, 朱小源, 苏菁 . 抗稻瘟病Pi2/9/z-t基因特异性分子标记的开发[J]. 中国水稻科学, 2015 , 29(4) : 305 -310 . DOI: 10．3969/j．issn．1001G7216．2015．03．010
Specific molecular markers for detecting the alleles of Pi2/Pi9/Piz-t, designated as Pi2SNP, Pi9SNP and Pizt-PA, respectively, have been successfully developed through sequence comparison method. These three specific markers can distinctly distinguish target genes from others mapped on Pi2/9 locus, which facilitate the molecular marker-assistant selection and the development of pyramiding cultivars combined with other allele R genes. Additionally, 101 main cultivars and breeding parents collected from different rice-planting areas have been diagnosed with these three specific markers, and the results showed that only 2 out of 101 cultivars carried Piz-t, but neither Pi2 nor Pi9 band had been detected. So far, it indicated that most of the main cultivars and breeding parents in China do not carry Pi2/Pi9/Piz-t. These results had provided important information for the application of Pi2/Pi9/Piz-t in the blast resistance breeding programs.
Key words： rice; blast; resistance gene; molecular marker; MAS; Pi2/9 locus
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