磷酸盐转运蛋白OsPT4影响水稻氮磷积累与利用的机理研究
#共同第一作者
收稿日期: 2020-08-03
修回日期: 2020-12-04
网络出版日期: 2021-11-10
基金资助
上海市青年英才扬帆计划资助项目(19YF1443200);上海市科技兴农重点攻关项目[沪农科推字(2020)2-1];国家青年自然科学基金资助项目(31902102);上海市农业科学院国家自然科学基金匹配项目[生科创-GT2020（匹配-3）];上海市农业科学院卓越团队计划资助项目[2017（A-03）]
Research on the Mechanisim of OsPT4 Regulating the Accumulation and Utilization of Nitrogen and Phosphorus in Rice
#These authors contributed equally to this work
Received date: 2020-08-03
Revised date: 2020-12-04
Online published: 2021-11-10
【目的】磷酸盐(Pi)转运蛋白OsPT4是水稻Pht1家族成员之一,负责Pi吸收以及向地上部的转运。探究OsPT4超表达对不同Pi条件下水稻氮(N)和磷(P)积累与利用的影响及其机理具有重要意义。【方法】以日本晴背景的OsPT4超表达株系为研究材料,通过设置正常供Pi与缺Pi的水培与桶培实验,检测生殖生长阶段不同组织中OsPT4的相对表达量,探究不同Pi处理条件下不同组织(叶片、叶鞘、茎秆、稻壳、穗柄和糙米)中的N和P浓度,并计算Pi吸收率及N和P利用效率,同时分析株高、单株产量、千粒重和结实率等产量构成因素。【结果】OsPT4在水稻生殖生长阶段的根系中相对表达丰度较高,OsPT4超表达使水稻剑叶、叶鞘、茎秆、稻壳、穗柄和糙米中的总P浓度不同程度提高,并显著提高了不同Pi处理条件下的Pi吸收与利用效率。同时,OsPT4的超表达可显著提高正常供Pi与缺Pi土壤条件下的单株产量与千粒重,以及缺Pi土壤中生长的结实率。除此之外,OsPT4的超表达使缺Pi条件下瘪壳与糙米中总N浓度平均升高16.8%和19.8%,N利用效率平均升高6.6%。【结论】OsPT4超表达不仅显著提高Pi吸收与利用效率,同时对不同Pi条件下的生理氮素利用率起促进作用。
孙雅菲, 宋科, 秦秦, 孙丽娟, 薛永 . 磷酸盐转运蛋白OsPT4影响水稻氮磷积累与利用的机理研究[J]. 中国水稻科学, 2021 , 35(6) : 565 -572 . DOI: 10.16819/j.1001-7216.2021.200803
【Objective】 The phosphate (Pi) transporter, OsPT4, is a member of the Pht1 family of rice, responsible for Pi uptake and transportation. It is important to explore the effect of OsPT4 overexpression on nitrogen (N) and phosphorous (P) accumulation and utilization in rice under different Pi conditions and its mechanism. 【Method】The OsPT4-overexpressing lines with Nipponbare background were used as material. Through the hydroponic and pot-cultivated experiments with (+Pi) or without Pi (-Pi) supply, the relative expression of OsPT4 in different tissues during reproductive growth stage was detected. The N and P concentrations in different tissues (leaf blade, leaf sheath, stem, rice hull, panicle axis and brown rice) under different Pi treatments were also measured. In addition, the Pi absorption rate and N/P use efficiency were calculated, and the plant height, yield per plant, 1000-grain weight and seed setting rate were analyzed. 【Result】The relative expression of OsPT4 was higher in rice roots during reproductive growth stage. Overexpression of OsPT4 increased the total P concentration in rice flag leaf blade, leaf sheaths, stems, rice hull, panicle axis and brown rice to varying degrees, and significantly improved the Pi absorption and use efficiency under different Pi regimes. Meanwhile, OsPT4 overexpression could significantly increase the yield per plant, 1000-grain weight and seed setting rate in +Pi and -Pi soils. In addition, the overexpression of OsPT4 also increased the total N concentration in unfilled rice hull and brown rice by 16.8% and 19.8%, and the N use efficiency by 6.6% under Pi-deficiency. 【Conclusion】The overexpression of OsPT4 not only promotes the Pi uptake and use efficiency, but also facilitates the physiology N use efficiency in different Pi conditions.
Key words： phosphorus; nitrogen; OsPT4; phosphorus use efficiency; nitrogen use efficiency
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