利用QTL-Seq定位粳稻整精米率QTL
#共同第一作者
收稿日期: 2020-11-16
修回日期: 2021-02-05
网络出版日期: 2021-09-10
基金资助
国家重点研发计划资助项目（2017YFD0300100）;河南省现代农业产业技术体系项目（S2012-04-G02）
QTL-Seq Mapping of Head Rice Rate QTLs in japonica Rice
#These authors contributed equally to this work
Received date: 2020-11-16
Revised date: 2021-02-05
Online published: 2021-09-10
【目的】稻米整精米率是加工品质的重要评价指标之一,其QTL定位将为提升稻米品质提供理论依据。【方法】通过两个粒型相近、整精米率差异极大的粳稻材料构建的F2分离群体为材料,利用QTL-Seq方法对分离群体中的高整精米率单株和低整精米率单株进行混池重测序以定位粳稻整精米率QTL位点。【结果】经过QTL-Seq分析发现在第8和第12染色体区间存在控制粳稻整精米率的QTL位点。进一步通过200个F2分离群体单株对第8和12染色体进行InDel分子标记QTL作图,发现粳稻中控制整精米率的QTL位点：qHRR8.1、qHRR8.2和qHRR12。其中,位于第8染色体21.8-23.2 Mb的qHRR8.1表型贡献率达到10.80%,其他两个QTL的贡献率较小。qHRR8.2位于第8染色体24.2-25.2 Mb,表型贡献率为3.26%。位于第12染色体的2.9-4.5 Mb的qHRR12表型贡献率为4.06%。【结论】本研究定位了1个控制粳稻整精米率的主效QTL位点qHRR8.1,对克隆粳稻整精米率控制基因以及在品质育种中提高粳稻整精米率有一定的参考价值。
陈喜娜, 袁泽科, 胡珍珍, 赵全志, 孙红正 . 利用QTL-Seq定位粳稻整精米率QTL[J]. 中国水稻科学, 2021 , 35(5) : 449 -454 . DOI: 10.16819/j.1001-7216.2021.201105
【Objective】 Rice milling quality is largely measured by head rice rate. So QTL mapping for head rice rate will lay a theoretical basis for rice quality improvement.【Method】 Using an F2 segregated population from two japonica rice cultivars with similar grain shape but different head rice rates, QTL-Seq bulked segregated sequencing method was used to locate the QTLs controlling head rice rate in japonica rice. 【Result】 Three QTLs were found on chromosomes 8 and 12 by QTL-Seq analysis, and the target regions were further verified by traditional QTL mapping method using 200 F2 individuals. Of the three QTLs, qHRR8.1 was located at 21.8-23.2 Mb with 10.80% phenotype variation contribution. The QTL qHRR8.2, located on chromosome 8 at 24.2-25.2 Mb and qHRR12, located on chromosome 12 at 2.9-4.5 Mb had smaller contribution of 3.26% and 4.06%, respectively. 【Conclusion】 The head rice rate controlling QTL qHRR8.1 was mapped on chromosome 8, which lay a theoretical foundation for further clone of head rice rate genes, and grain quality improvement in japonica rice.
Key words： rice; grain milling quality; head rice rate; QTL mapping
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