水稻白化转绿和穗顶端退化突变体vpa1的遗传分析和基因定位
收稿日期: 2020-07-02
修回日期: 2020-07-27
网络出版日期: 2021-01-10
基金资助
水稻生物学国家重点实验室自主课题资助项目(2017ZZKT10106)
Genetic Analysis and Gene Mapping of a Virescent and Panicle AbortionMutant vpa1in Rice
Received date: 2020-07-02
Revised date: 2020-07-27
Online published: 2021-01-10
王中豪, 贺彦, 张晓波, 徐霞, 吴建利, 施勇烽 . 水稻白化转绿和穗顶端退化突变体vpa1的遗传分析和基因定位[J]. 中国水稻科学, 2021 , 35(1) : 19 -26 . DOI: 10.16819/j.1001-7216.2021.0702
【Objective】Genes related to chloroplast development and panicle phenotype can be identified through the study onvirescent mutants and panicle apical abortion (paa) mutants in rice. 【Method】The main agronomic traits of vpa1 (virescent and panicle abortion 1) mutant and the wild-type were compared under conventional planting conditions in field. The virescent gene and paagene were mapped by bulked segregant analysis and map-based cloning.【Result】vpa1exhibited albino leaves at seedling stage andthe leaves graduallyreturned to normal. During heading stage,vpa1showed degeneration of spikelets at the tops of panicles. Except seed-setting rate, the agronomic traits including plant height, panicle length, number of filled grains per panicle and 1000-grain weight decreased significantly in vpa1as compared with the wild-type.Genetic analysis indicated that both the virescentphenotypeand the paa phenotype of vpa1are controlled by single recessive nuclear gene, and the virescentgene and paa gene were segregated independently.The virescent gene, Osv16, was mapped to a 125kb region between RM3441 and RM3029 on chromosome 3. Thepaagene, Ospaa10, was mapped to a 190kb region between RM11157 and RM5972 on chromosome 1.【Conclusion】mutations ofOsv16 and Ospaa10 causedsimultaneous variation of leaf color and spikelet phenotype in vpa1. Osv16 and Ospaa10are likely two new genes as no other virescent and paa genes have been identified in the related region, further cloning of both genes are currently underway.
Key words： rice; virescent; panicle apical abortion; gene mapping
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