水稻抗性蛋白OsRRK1抗褐飞虱机理分析
收稿日期: 2020-04-25
修回日期: 2020-05-18
网络出版日期: 2020-11-10
基金资助
湖南省自然科学基金青年项目（2019JJ50281）;湖南省教育厅优秀青年项目（18B455）;杂交水稻国家重点实验室（武汉大学）开放课题基金资助项目(KF201901);娄底市应用技术与开发项目（33319013）;湖南省应用特色学科（植物保护）资助项目;湖南人文科技学院卓越人才培养计划资助项目（校教通 2017 第 74 号）;湖南省高等学校科技创新团队(201937924)
Mechanism Analysis of Rice Resistance Protein OsRRK1 Againstthe Brown Planthopper
Received date: 2020-04-25
Revised date: 2020-05-18
Online published: 2020-11-10
马银花, 李萍芳, 董文静, 易松望, 杨芳, 杜波, 金晨钟 . 水稻抗性蛋白OsRRK1抗褐飞虱机理分析[J]. 中国水稻科学, 2020 , 34(6) : 512 -519 . DOI: 10.16819/j.1001-7216.2020.0406
【Objective】OsRRK1 (Rop-interacting receptor-like kinase 1) protein in rice is the first RLCK VI family protein in rice that has been studied.It plays an important role inregulating the curling of rice leaves and the defense against brown planthoppers. However, the mechanism of its regulation of resistance to the brown planthopperremains unclear. Weattempt to further explore the mechanism of OsRRK1gene resistance to the brown planthoppers.【Method】The interaction between OsRRK1 protein and OsLecRK (lectin-like receptor kinase), OsLecRK1, OsLecRK2 and OsLecRK3 was analyzed by yeast two-hybrid method.OsLecRK is a candidate gene forBPH15 range of anti-brown planthopper, and is an important member of rice innate immune system. OsLecRK is involved innot only rice innate immune response, including resistance to brown planthoppers, white leaf blight and rice blast, but alsothe development of rice. The gene clusters of OsLecRK1, OsLecRK2 and OsLecRK3 constitute the anti-brown planthopper gene BPH3. Rice containing the BPH3 gene has a broad spectrum and lasting insect resistance. Meanwhile, DNAMAN software was used to analyze the homology of OsLecRK with OsLecRK1, OsLecRK2 and OsLecRK3.【Result】The analysis results of DNAMAN showed that OsLecRK has high homology with OsLecRK1, OsLecRK2 and OsLecRK3, and the proteinsshared more than 50% identity.The results of yeast two-hybrid analysis showed that OsRRK1 interacted with OsLecRK, OsLecRK2 and OsLecRK3.【Conclusion】OsRRK1 participates in the process of resistance to the brownplanthoppersthroughinteracting with BPH15 and BPH3 candidate genes.
Key words： rice; OsRRK1; brown planthopper; yeast two-hybrid system
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