水稻蔗糖转运蛋白OsSUT4参与蔗糖转运的功能研究
收稿日期: 2020-04-02
修回日期: 2020-05-27
网络出版日期: 2020-11-10
基金资助
国家自然科学基金资助项目（31901454）;江苏省现代作物生产协同创新中心
Function Analysis of Sucrose Transporter OsSUT4 in Sucrose Transport in Rice
Received date: 2020-04-02
Revised date: 2020-05-27
Online published: 2020-11-10
关键词： 水稻; CRISPR/Cas9; 基因编辑; 蔗糖转运; 蔗糖转运蛋白
李孟珠, 王高鹏, 巫月, 任怡, 李刚华, 刘正辉, 丁艳锋, 陈琳 . 水稻蔗糖转运蛋白OsSUT4参与蔗糖转运的功能研究[J]. 中国水稻科学, 2020 , 34(6) : 491 -498 . DOI: 10.16819/j.1001-7216.2020.0316
【Objective】The transmembrane transport of sucrose is mainly mediated by sucrose transporters, among which five SUT coding genes have been identified inrice. However, the role of OsSUT4 in the sucrose transport is unclear. 【Method】CRISPR/Cas9 techniques were used to construct ossut4-deletedmutants. The ossut4 mutant was featured by lower plant height and grain yield as well as higher tiller number as compared withwildtype plants.【Result】Sucrose and starchaccumulationin the leaf of the ossut4 mutants lead to slower net photosynthetic rate; insufficient sugar supply results in the postponeof grain filling. Base on the subcellular and histochemical localization,OsSUT4 was located on the cytoplasmic membrane. Tissue localization showed that OsSUT4 was expressed in the embryo of germinating seed, vascular bundles of coleoptile, glume shell, anthers at flowering stage and aleurone layer of caryopsis atfilling stage. 【Conclusion】OsSUT4 plays an important role in sucrose transport.
Key words： rice; CRISPR/Cas9; gene editing; sucrose transport; sucrose transporter
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