利用剩余杂合体衍生的近等基因系精细定位水稻粒长微效QTL qGL1.1
收稿日期: 2019-11-21
修回日期: 2019-12-28
网络出版日期: 2020-03-10
基金资助
国家重点研发计划资助项目(2017YFD0100305);中央公益性科研院所基本科研业务费专项(2017RG001-2)
Fine Mapping of qGL1.1, a Minor QTL for Grain Length, Using Near Isogenic Lines Derived from Residual Heterozygotes in Rice
Received date: 2019-11-21
Revised date: 2019-12-28
Online published: 2020-03-10
【目的】本研究旨在对前期在水稻第1染色体长臂521.8 kb的区间内定位到的qTGW1.1b进行精细定位。【方法】从qTGW1.1和qTGW1.2所在区间分别呈杂合的2个BC2F9单株配组衍生的F4群体中,筛选到Wn28826- RM1231区间内杂合片段呈梯系排列的3个单株,构建了3套F5:6近等基因系。2017年种植于浙江杭州,考查千粒重、粒长和粒宽。利用SAS软件的GLM程序进行双因素方差分析,对qTGW1.1b的效应进行了验证。在此基础上,筛选出杂合片段更小且呈交迭排列的6个剩余杂合体,发展了6套F8:9近等基因系,2018年种植于海南陵水。对每套近等基因系中双亲基因型株系的表型差异进行双因素方差分析。【结果】qTGW1.1b在2个试验中对粒长和千粒重均呈极显著差异,效应方向一致且大小稳定。密阳46等位基因能分别增加粒长0.027 mm和提高千粒重0.17 g,贡献率分别达到27.12%和19.09%。【结论】鉴于qTGW1.1b在前后试验中对粒长影响最为显著,而对粒宽作用不显著,故将qTGW1.1b重新命名为qGL1.1。通过比较各套近等基因系的分离区间的基因组位置,最终将qGL1.1定位于Wn29077和Wn29154之间约76.8 kb的区间内。
李盼盼, 朱玉君, 郭梁, 庄杰云, 樊叶杨 . 利用剩余杂合体衍生的近等基因系精细定位水稻粒长微效QTL qGL1.1[J]. 中国水稻科学, 2020 , 34(2) : 125 -134 . DOI: 10.16819/j.1001-7216.2020.9125
【Objective】The objective of this study is to fine-map qTGW1.1b which was previously mapped in a 521.8-kb region on the long arm of rice chromosome 1.【Method】In this study, two BC2F9 plants carrying qTGW1.1 and qTGW1.2, respectively, was crossed and produced an F4 population. Three plants carrying sequential heterozygous segments in the interval Wn28826-RM1231 were selected and selfed to develop three sets of near isogenic lines (NILs) comprising the two homozygous genotypes. The NILs in F5:6 were planted in Hangzhou in 2017 and the 1000-grain weight, grain length and grain width were measured. The effect of qTGW1.1b was validated by two-way analysis of variance (ANOVA) using SAS program. Then six new sequential residual heterozygotes carrying smaller heterozygous segments overlapped in the interval Wn28826-RM1231 were identified. Six sets of pairwise NILs in F8:9 were planted in Lingshui in 2018 and the three traits were measured. Phenotypic differences between the two homozygous genotypic groups were analyzed using ANOVA.【Results】The allelic direction of qTGW1.1b remained consistent and the genetic effects were stable in the two trials. The Milyang 46 allele could significantly increase grain length and 1000-grain weight by 0.027 mm and 0.17 g with the contribution up to 27.12% and 19.09%, respectively.【Conclusion】Considering that qTGW1.1b stably showed significant effect to grain length but not to grain width in both previous and this study, qTGW1.1b was renamed as qGL1.1. As a result, qGL1.1 was delimited into a 76.8-kb region franked by Wn29077 and Wn29154 based on comparing of the segregating regions among the six sets of NILs.
Key words： rice; near isogenic line; minor effect; grain length; fine mapping
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