应用环介导等温扩增技术检测江苏水稻种子携带的水稻恶苗病菌
收稿日期: 2017-09-26
修回日期: 2017-12-13
网络出版日期: 2018-09-10
基金资助
国家863计划资助项目(2012AA101501)
Detection of Seed-borne Rice Bakanae Pathogens in Jiangsu Province, China Using Loop-mediated Isothermal Amplification Assays
Received date: 2017-09-26
Revised date: 2017-12-13
Online published: 2018-09-10
【目的】本研究旨在了解江苏省水稻主栽品种种子携带水稻恶苗病原菌的情况。【方法】采用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)对江苏65个水稻主栽品种种子携带藤仓镰孢、层出镰孢、拟轮枝镰孢和Fusarium andiyazi等4种水稻恶苗病菌进行检测。【结果】65个品种中有54个品种的种子样本携带恶苗病菌,表明江苏省稻种携带水稻恶苗病菌是一种普遍现象。其中,藤仓镰孢为优势种,检出率高达83.07%,其次是层出镰孢和Fusarium andiyazi,检出率分别为12.30%、6.15%,但供试的65个品种种子均未检测到携带有拟轮枝镰孢。【结论】该技术可用于水稻种子携带恶苗病菌的快速检测。
袁咏天, 戎振洋, 叶文武, 张红生, 庄义庆, 郑小波 . 应用环介导等温扩增技术检测江苏水稻种子携带的水稻恶苗病菌[J]. 中国水稻科学, 2018 , 32(5) : 493 -500 . DOI: 10.16819/j.1001-7216.2018.7119
【Objective】The research aims to reveal the situation of seed-borne rice bakanae pathogens in Jiangsu Province, China. 【Methods】The seeds of 65 major rice cultivars collected from this area were analyzed by using loop-mediated isothermal amplification (LAMP) assays for specific detection of 4 known rice bakanae pathogen species, including Fusarium fujikuroi, F. proliferatum, F. verticilliodes and F. andiyazi. 【Results】There were 54 rice cultivars carrying rice bakanae pathogens, indicating it’s a common phenomenon that rice seeds carrying rice bakanae pathogens in Jiangsu Province. And the most frequent species was F. fujikuroi, representing 83.07% of the total samples tested, followed by F. proliferatum and F. andiyazi, representing 10% and 6% respectively. However, none of the 65 rice cultivars was tested to be carrying F. verticilliodes. 【Conclusions】The method is readily available for detecting seed-borne rice bakanae pathogens.
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