一个水稻铜锌SOD酶基因在应答亚砷酸盐胁迫中的作用
收稿日期: 2017-12-25
修回日期: 2018-02-01
网络出版日期: 2018-09-10
基金资助
国家自然科学基金资助项目(31471431);浙江农林大学“青年拔尖人才”计划资助项目;浙江农林大学学生科研训练项目(2013200014, 2013200037, 2013200056)
Functional Analysis of a Copper/Zinc SOD Encoding Gene in Response to Arsenite Stress in Rice
Received date: 2017-12-25
Revised date: 2018-02-01
Online published: 2018-09-10
【目的】水稻Os08g44770.1基因编码一个铜锌SOD酶,但其在响应亚砷酸盐[As(Ⅲ)]胁迫中的生物学功能未知。本研究旨在深入揭示由该基因调控水稻砷耐性改变的分子机理并为水稻抗逆育种提供理论参考。【方法】以野生型日本晴(WT)和2个Os08g44770.1过表达转基因株系为试材,通过胁迫处理、生理指标测定和基因表达分析等,系统探究了转基因植株对As(Ⅲ)的耐受性表现,并初步揭示了其调控水稻砷耐性的生理和分子机理。【结果】与WT相比,过表达转基因株系对As(Ⅲ)更加敏感;转基因植株在砷胁迫下的根系相对伸长量、生物量(干质量)、叶绿素含量、根系细胞质膜完整性、叶片抗氧化程度等均显著低于WT;Os08g44770.1在砷处理后的WT和转基因植株叶片中的表达模式略有不同,但均表现为处理24 h时被显著诱导表达。【结论】过度表达Os08g44770.1基因可导致水稻的砷耐受性极显著下降。
窦玲玲, 胡海超, 马龙, 柯笑楠, 刘明月, 练旺民, 金珂, 谢玲娟, 刘庆坡 . 一个水稻铜锌SOD酶基因在应答亚砷酸盐胁迫中的作用[J]. 中国水稻科学, 2018 , 32(5) : 437 -444 . DOI: 10.16819/j.1001-7216.2018.7154
【Objective】Os08g44770.1 encodes a Cu/Zn-SOD protein in rice, but its biological function in response to arsenite [As(Ⅲ)] remains unclear. The results presented here would be intriguing for further investigating the molecular mechanisms of Os08g44770.1-mediated arsenic tolerance in rice, and also providing useful new idea for rice stress resistance breeding. 【Methods】Using wild type(WT) Nipponbare and two transgenic rice lines overexpressing Os08g44770.1 as materials, we investigated the phenotypes of WT and transgenic plants after exposure to As(Ⅲ), and further uncovered its physiological and molecular mechanisms in regulation of As(Ⅲ) tolerance in rice through stress treatment, physiological index measurement, and gene expression analysis, etc. 【Result】The results showed that, compared with WT, transgenic rice plants were more sensitive to As(Ⅲ) stress; Under As(Ⅲ) exposure, transgenic plants showed shorter relative root elongation, lower biomass (dry weight) and chlorophyll content, as well as lower root cell membrane integrity and antioxidant abilities in leaves. Moreover, Os08g44770.1 exhibited slightly different expression patterns but both showing expression peak at 24 h after As(Ⅲ) treatment in leaves of WT and transgenic plants. 【Conclusion】Strong overexpression of Os08g44770.1 in rice would result in its hypersensitivity to arsenite stress.
Key words： rice; Cu/Zn SOD; arsenite; transgenic; physiological mechanism; gene expression
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