水稻蛋白激酶基因启动子OsSRLp的分离及特性分析
收稿日期: 2017-10-26
修回日期: 2017-12-28
网络出版日期: 2018-07-10
基金资助
山东省自然科学基金面上项目(2016ZRC02178);山东省自然科学基金资助项目(ZR2014CQ024);山东省农业科学院青年英才计划资助项目(2016-2018);山东省农业科学院重大科技成果培育计划资助项目(2015CGPY10);国家重点研发计划资助项目(2016YFD0100903)
Isolation and Characteristic Analysis of Protein Kinase Promoter OsSRLp from Rice
Received date: 2017-10-26
Revised date: 2017-12-28
Online published: 2018-07-10
【目的】为研究水稻中I型酪蛋白激酶基因OsSRL启动子的结构及功能,【方法】以水稻中花11基因组DNA为模板,经PCR扩增获得基因上游约1600 bp序列,命名为启动子OsSRLp。应用PLACE在线软件,分析序列中的顺式作用元件,同时构建含有GUS报告基因的植物表达载体,转化水稻。【结果】OsSRL为组成型表达,OsSRLp含有调控生殖发育、激素应答及逆境响应等多种顺式作用元件。OsSRLp驱动的GUS报告基因在根、茎中均有所表达,在小穗中表达丰度较高,而在其他组织中表达丰度较低。【结论】OsSRLp为组成型启动子,其下游调控基因OsSRL可能参与水稻发育及生殖过程。
王庆国, 王莹莹, 李臻, 潘教文, 王一帆, 李颖秀, 刘炜 . 水稻蛋白激酶基因启动子OsSRLp的分离及特性分析[J]. 中国水稻科学, 2018 , 32(4) : 335 -341 . DOI: 10.16819/j.1001-7216.2018.7127
【Objective】To study the construction and function of OsSRL, a typeⅠcasein kinase, 【Method】OsSRL and about 1600 bp up-stream of the gene named as OsSRLp were isolated using genomic DNA of rice Zhonghua 11. The regulatory elements of OsSRLp were analyzed using online software of PLACE, and the results showed that several key cis-elements which associated with reproductive process, signal and stress response were included. 【Results】The expression pattern analysis showed that OsSRL was constitutively expressed in different tissues of rice. The fusion expression vector of OsSRLp and GUS was constructed, and the vector was transformed into rice callus. After positive transgenic lines were obtained, the GUS activities were further detected, and the results showed that relative higher level expression was detected in spikelet, root and stem, as well as low expression levels in other rice tissues. The results indicated that OsSRLp possess constitutive activities with slightly variations in different rice tissues. 【Conclusion】 Combined with the characteristic elements and their potential functions of OsSRLp, the results implied that OsSRL and its promoter OsSRLp could participate in and regulate the development and reproduction progresses of plants.
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