水稻矮缩病毒对介体昆虫黑尾叶蝉生物学参数及种群增长的影响
收稿日期: 2017-08-02
修回日期: 2017-09-28
网络出版日期: 2018-01-10
基金资助
国家973计划资助项目（2014CB138404）;国家转基因生物新品种培育重大专项（2016ZX08011-001）
Influence of Rice dwarf virus on Biological Parameters and Population Growth of Vector Insect Nephotettix cincticeps (Uhler) (Hemiptera: Cicadellidae)
Received date: 2017-08-02
Revised date: 2017-09-28
Online published: 2018-01-10
【目的】 水稻矮缩病毒(RDV)是水稻普通矮缩病的病原。该病害主要依赖于黑尾叶蝉经卵以持久增殖方式传播,危害水稻。本研究旨在明确RDV对黑尾叶蝉存活、发育、繁殖和种群增长的影响,分析病毒对介体昆虫的间接影响。【方法】 采用室内实验比较了黑尾叶蝉在健康水稻及感病水稻上的发育繁殖情况,组建了实验种群生命表,并调查了RDV对种群增长的影响。【结果】 与健康水稻相比,感病水稻上叶蝉若虫期存活率更高,雌若虫发育历期显著缩短,雄成虫寿命显著延长,产卵量显著增加,其他生物学参数无显著差异。五个生命表参数仅净生殖率(R0)存在显著差异,感病水稻上叶蝉R0显著高于健康水稻。饲喂感病水稻的养虫笼内叶蝉种群增长更快,总成虫数量更多,在接虫后的第4、5和6月显著高于健康水稻上的数量。【结论】 水稻矮缩病毒可以提高黑尾叶蝉的存活率和产卵量,并促进种群的增长。
王前进, 党聪, 方琦, 叶恭银 . 水稻矮缩病毒对介体昆虫黑尾叶蝉生物学参数及种群增长的影响[J]. 中国水稻科学, 2018 , 32(1) : 89 -95 . DOI: 10.16819/j.1001-7216.2018.7090
【Objective】 The rice dwarf virus (RDV), a phytoreovirus in the family Reoviridae, is the pathogeny of rice dwarf virus disease. RDV is mainly transmitted by green rice leafhoppers, Nephotettix cincticeps (Uhler) (Hemiptera: Cicadellidae), in a persistent-propagative manner and is transovarially transmitted to offspring and caused great decreases to rice yields. Our aim is to study the effects of RDV on leafhoppers survival, development, reproduction and population growth, and to explore the “double” damage mechanism by RDV and vector green rice leafhoppers and finally to reveal the mechanism within the tritrophic relationship.【Method】 We compared the biological parameters of the green rice leafhoppers on RDV-infected and non-infected rice plants, life table of experimental population of leafhopper was established. We also analyzed the influence of RDV on green rice leafhoppers population growth.【Result】 Compared with healthy rice plants, female nymphal duration was significantly shorter, nymph survival, male adult longevity and fecundity were significantly longer/higher on RDV-infected rice plants. Other biological parameters were not largely affected. Among the five life table parameters, only net reproductive rates (R0) was significantly affected, it was largely higher on RDV-infected rice plants compared with healthy rice plants. The green rice leafhopper population grew faster on RDV-infected rice plants and the total number of adults was significantly higher than healthy rice plants at the 4th, 5th and 6th month after inoculation.【Conclusion】 RDV could enhance survival rate, fecundity and accelerate population growth of green rice leafhoppers.
| [1] | 稻麦重要病毒病株系鉴定和防控技术体系研究课题组//稻麦主要病毒病识别与控制.北京:中国农业出版社, 2011:15-26. |
| [1] | Research group of virus strains identification and prevention technology onrice andwheat//Identification andControl of Main Viruses on Rice and Wheat. Beijing: China Agriculture Press, 2011: 15-26. (in Chinese) |
| [2] | 朱勇,李婷婷,董家红,丁铭,张仲凯.云南水稻矮缩病毒病调查监测初报.西南农业学报,2011,24(6):2181-2184. |
| [2] | Zhu Y,Li T T,Dong J H, D M, Zhang Z K. Primary report on investigating and testing of rice dwarf virus disease in Yunnan Province.Southwest China J Agric Sci,2011,24(6):2181-2184. (in Chinese with English abstract) |
| [3] | 宛柏杰,林文武,吴锦鸿,陈晓敏,吴祖建,张洁.水稻矮缩病毒非结构蛋白Pns6 和外壳蛋白P8多克隆抗体的制备及其应用.福建农林大学学报:自然科学版,2016,45(1):42-47. |
| [3] | Wan B J,Lin W W,Wu J H,Chen X M,Wu Z J,Zhang J.Preparation and application comparision of polyclonal antibodies against non-structral protein Pns6 and coat protein P8 ofRice dwarf virus. J Fujian Agric Fores U: Nat Sci Ed,2016,45(1):42-47. (in Chinese with English abstract) |
| [4] | 蔡晶,李西明,季芝娟,马良勇,杨长登.我国水稻普通矮缩病的研究进展.中国稻米,2009,15(1):10-15. |
| [4] | Cai J,Li X M,Ji Z J,Ma L Y,Yang C D.Research advances on rice dwarf disease in China.China Rice,2009,15(1):10-15. (in Chinese) |
| [5] | Zheng H H,Yu L,Wei C H,Hu D W,Shen Y D,Chen Z L,Li Y.Assembly of double-shelled, virus-like particles in transgenic rice plants expressing two major structural proteins of rice dwarf virus.J Virol,2000,74(20):9808-9810. |
| [6] | Nakagawa A,Miyazaki N,Taka J,Naitow H,Ogawa A,Fujimoto Z,Mizuno H,Higashi T,Watanabe Y,Omura T.The atomic structure of rice dwarf virus reveals the self-assembly mechanism of component proteins.Structure: London, England,2003,11(10):1227-1238. |
| [7] | 顾永林.水稻矮缩病的研究.农业灾害研究,2012,2(1):1-5. |
| [7] | Gu Y L.Research on rice dwarf disease.J Agric Catastrophol,2012,2(1):1-5. (in Chinese with English abstract) |
| [8] | Omura T,Yan J,Zhong B X,Wada M,Zhu Y F,Tomaru M,Maruyama W,Kikuchi A,Watanabe Y,Kimura I,Hibino H.The P2 protein of rice dwarf phytoreovirus is required for adsorption of the virus to cells of the insect vector.J Virol,1998,72(11):9370-9373. |
| [9] | Omura T,Yan J.Role of outer capsid proteins in transmission of phytoreovirus by insect vectors.Adv Virus Res,1999,54: 15-43. |
| [10] | Stout M J,Thaler J S, Thomma B P H J. Plant-mediated interactions between pathogenic microorganisms and herbivorous arthropods.Annu Rev Entomol,2006,51: 663-689. |
| [11] | Colvin J,Omongo C A,Govindappa M R,Stevenson P C,Maruthi M N,Gibson G,Seal S E,Muniyappa V.Host-plant viral infection effects on arthropod-vector population growth, development and behavior: management and epidemiological implications.Adv Virus Res,2006,67(6):419-452. |
| [12] | Belliure B,Janssen A,Maris P C,Peters D,Sabelis M W.Herbivore arthropods benefit from vectoring plant viruses.Ecol Lett,2005,8: 70-79. |
| [13] | Hogenhout S A,Ammar E D,Whitfield A E,Redinbaugh M G.Insect vector interactions with persistently transmitted viruses.Annu Rev Phytopathol,2008,46(1):327-359. |
| [14] | Maris P C,Joosten N N,Goldbach R W,Peters D.Tomato spotted wilt virus infection improves host suitability for its vector,Frankliniella occidentalis. Phytopathology,2004,94(7):706-711. |
| [15] | Jiu M,Zhou X P,Tong L,Xu J,Jiang X,Wan F H,Liu S S.Vector-virus mutualism accelerates population increase of an invasive whitefly.PLoS ONE,2007,2(1):e182. |
| [16] | Guo J Y,Ye G Y,Dong S Z,Liu S S.An invasive whitefly feeding on a virus-infected plant increased its egg production and realized fecundity.PLoS ONE,2012,5(7):e11713. |
| [17] | Guo J Y,Dong S Z,Yang X L,Cheng L,Wan F H,Liu S S,Zhou X P,Ye G Y.Enhanced vitellogenesis in a whitefly via feeding on a begomovirus-infected plant.PLoS ONE,2012,7(8):e43567. |
| [18] | 梁莉,郭建洋,田俊策,陈洋,胡萃,叶恭银.水稻矮缩病毒对黑尾叶蝉卵巢发育与产卵量的影响.植物保护学报,2010,37(4):375-376. |
| [18] | Liang L,Guo J Y,Tian J C,Chen Y,Hu C,Ye G Y.Effects of rice dwarf virus on ovarian development and fecundity of the green leafhopper,Nephotettix cincticeps(Fabricius). Acta Phytophyl Sin,2010,37(4):375-376. (in Chinese) |
| [19] | Zhang J,Zheng X,Chen Y D,Hu J,Dong J H,Su X X,Zhang Z K.Southern rice black-streaked dwarf virus infection improves host suitability for its insect vector,Sogatella furcifera(Hemiptera: Delphacidae). J Econ Entomol,2014,107(1):92-97. |
| [20] | Liu J,Zhao H,Jiang K,Liu S S.Differential indirect effects of two plant viruses on an invasive and an indigenous whitefly vector: Implications for competitive displacement.Ann Appl Biol,2009,155(3):439-448. |
| [21] | Nakasuji F,Kiritani K.Ⅲ. Effects of rice dwarf virus upon its vector,Nephotettix circticeps Uhler (Hemiptera: Deltocephalidae), and its significance for changes in relative abundance of infected individuals among vector populations. Appl Entomol Zool,1970,5(1):1-12. |
| [22] | Fiebig M,Poehling H M,Borgemeister C.Barley yellow dwarf virus, wheat, andSitobion avenae: A case of trilateral interactions. Entomol Exp Appl,2004,110(1):11-21. |
| [23] | Sinisterra X H, McKenzie C L, Hunter W B, Powell C A, Shatters R G Jr. Differential transcriptional activity of plant-pathogenic begomoviruses in their whitefly vector (Bemisia tabaci, Gennadius: Hemiptera Aleyrodidae). J Gen Virol,2005,86(5):1525-1532. |
| [24] | Mann R,Sidhu J,Butter N A,Sohi A S,Sekhon P S.Performance ofBemisia tabaci (Hemiptera: Aleyrodidae) on healthy and cotton leaf curl virus infected cotton. Fla Entomol,2008,91(2):249-255. |
| [25] | Tu Z,Ling B,Xu D L,Zhang M X,Zhou G H.Effects of southern rice black-streaked dwarf virus on the development and fecundity of its vector,Sogatella furcifera. Virol J,2013,10(1):145. |
| [26] | Lei W B,Liu D F,Li P,Hou M L.Interactive effects of southern rice black-streaked dwarf virus infection of host plant and vector on performance of the vector,Sogatella furcifera(Homoptera: Delphacidae).J Econ Entomol,2014,107(5):1721-1727. |
| [27] | Wijkamp I,Goldbach R,Peters D.Propagation of tomato spotted wilt virus inFrankliniella occidentalis does neither result in pathological effects nor in transovarial passage of the virus. Entomol Exp Appl,1996,81(3):285-292. |
| [28] | Lapidot M,Friedmann M,Pilowsky M,Ben-Joseph R,Cohen S.Effect of host plant resistance to tomato yellow leaf curl virus (TYLCV) on virus acquisition and transmission by its whitefly vector.Phytopathology,2001,91(12):1209-1213. |
| [29] | Matsuura S,Hoshino S.Effect of tomato yellow leaf curl disease on reproduction ofBemisia tabaci Q biotype (Hemiptera: Aleyrodidae) on tomato plants. Appl Entomol Zool,2009,44(1):143-148. |
| [30] | Wang Q J,Han N S,Dang C,Lu Z B,Wang F,Yao H W,Peng Y F,Stanley D,Ye G Y.Combined influence of Bt rice and rice dwarf virus on biological parameters of a non-target herbivore,Nephotettix cincticeps (Uhler), 2017,12(7):e0181258. |
| [31] | 陈茂. 转基因抗虫、抗病毒水稻对非靶标生物的生态安全性评价.杭州:浙江大学. 2005. |
| [31] | Chen M.Ecological safety assessments of transgenic rice with insect or virus resistance on their non-target organisms. Hangzhou: Zhejiang University,2005. () |
| [32] | Yoshida S,Forno D A,Cock J H.Laboratory manual for physiological studies of rice. The International Rice Research Institute, Manila, Philippines,1976: 61-66. |
| [33] | Hibino H.Biology and epidemiology of rice viruses.Annu Rev Phytopathol,1996,34(4):249-274. |
| [34] | SPSS. SPSS for Windows User’s Guide Release 16. SPSS Inc., Chicago,2007. |
| [35] | 徐汝梅. 昆虫种群生态学.北京:北京师范大学出版社,1987:97-107. |
| [35] | Xu R M.The ecology of insect population.Beijing:Normal University Press, 1987: 97-107. (in Chinese) |
| [36] | Maia A H,Luiz A J,Campanhola C.Statistical inference on associated fertility life table parameters using jackknife technique: computational aspects.J Econ Entomol,2000,93(2):511-518. |
| [37] | Blua M J,Perring T M,Madore M A.Plant virus-induced changes in aphid population development and temporal fluctuations in plant nutrients.J Chem Ecol,1994,20(3):691-707. |
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