应用东乡野生稻回交重组自交系群体分析糙米矿质含量QTL
收稿日期: 2017-01-19
修回日期: 2017-04-02
网络出版日期: 2018-01-10
基金资助
国家863计划资助项目(2014AA10A604);江西省重点研发计划重大专项(20161ACF60022);江西省自然科学基金资助项目(20171ACB21071);江西省农业科学院创新基金资助项目(20161CBS002)
QTL Analysis for Mineral Contents in Brown Rice Using a BC2F4:5 Population Derived from Dongxiang Wild Rice (Oryza rufipogon Griff.)
Received date: 2017-01-19
Revised date: 2017-04-02
Online published: 2018-01-10
【目的】 强化粮食作物的必需矿物质有利于缓解人们矿质营养缺乏症。【方法】 从协青早B//协青早B/东乡野生稻BC1F5群体中挑选到1个单株A58,与协青早B回交,构建了BC2F4:5群体。采用电感耦合等离子体原子发射光谱仪ICP-AES测定132个BC2F4:5株系的糙米Mg、Ca、Zn、Fe、Mn和Cu含量,应用Windows QTL Cartographer 2.5进行糙米矿质QTL分析。【结果】 共检测到17个糙米矿质含量QTL,分别位于第1、4、6、8、9和11等6条染色体上,包括Mg含量1个、Ca含量4个、Zn含量4个、Fe含量2个、Mn含量2个和Cu含量4个。这些QTL解释表型变异的5.0%~47.2%,其中8个QTL的增效等位基因来自东乡野生稻。【结论】 12个QTL聚集于5条染色体上的5个QTL簇,表明不同矿质营养元素涉及到共同遗传生理机制,可通过分子标记辅助选择方法将有利等位基因应用于稻米营养品质改良。
胡标林, 黄得润, 肖叶青, 何强生, 万勇, 樊叶杨 . 应用东乡野生稻回交重组自交系群体分析糙米矿质含量QTL[J]. 中国水稻科学, 2018 , 32(1) : 43 -50 . DOI: 10.16819/j.1001-7216.2018.7011
【Objective】 Biofortifying food crops with essential minerals would alleviate mineral deficiencies in humans.【Method】 One plant A58 was selected from Xieqingzao B// Xieqingzao B /Dongxiang wild rice BC1F5 population, and was backcrossed with Xieqingzao B to develop a BC2F4:5 population. The contents of Mg, Ca, Zn, Fe, Mn and Cu in brown rice of 132 BC2F4:5 lines were measured with an inductively coupled plasma atomic emission spectrometer (ICP-AES). Detection of quantitative trait loci (QTLs) for mineral contents in brown rice was conducted using Windows QTL Cartographer 2.5.【Result】 A total of 17 QTLs for mineral contents in brown rice were detected on chromosomes 1, 4, 6, 8, 9 and 11, respectively, including one for Mg content, four for Ca content, four for Zn content, two for Fe content, two for Mn content, and four for Cu content. The explained phenotypic variations ranged from 5.0% to 47.2%, and eight QTLs of them had the enhancing alleles derived from Oryza rufipogon. 【Conclusion】 Twelve QTLs were clustered in five chromosomal regions, indicating that common genetic-physiological mechanisms were involved for different mineral nutrients, and the beneficial alleles could be utilized to improve grain nutritional quality by marker-assisted selection.
Key words： Dongxiang wild rice; brown rice; mineral content; QTL mapping
| [1] | Stein A J.Global impacts of human mineral malnutrition.Plant Soil,2010,335(1/2):133-154. |
| [2] | White P J,Brown P H.Plant nutrition for sustainable development and global health.Ann Bot,2010,105(7):1073-1080. |
| [3] | Rawat N,Neelam K,Tiwari V K,Dhaliwal H S.Biofortification of cereals to overcome hidden hunger.Plant Breeding,2013,132: 437-455. |
| [4] | Bashir K,Takahashi R,Nakanishi H,Nishizawa N K.The road to micronutrient biofortification of rice: Progress and prospects.Front Plant Sci,2013,4: 15. |
| [5] | Bhullar N K,Gruissem W.Nutritional enhancement of rice for human health: The contribution of biotechnology.Biotech Adv,2013,31(1):50-57. |
| [6] | Gregorio G B,Senadhira D,Htut T.Breeding for trace mineral density in rice.Food Nutr Bull,2000,21(4):382-386. |
| [7] | Srinivasa J,Arun B,Mishra V K,Singh G P,Velu G,Babu R,Vasistha N K,Joshi A K.Zinc and iron concentration QTL mapped in aTriticum spelta × T. aestivum cross. Theor Appl Genet,2014,127(7):1643-1651. |
| [8] | Šimić D,Mladenovic D S,Zdunic Z,Jambrović A,Ledencan T,Brkić J,Brkić A,Brkić I.Quantitative trait loci for biofortification traits in maize grain.J Hered,2012,103: 47-54. |
| [9] | Zhang M, Pinson S R M, Tarpley L, Huang X Y, Lahner B, Yakubova E, Baxter I O, Guerinot M L, Salt D E. Mapping and validation of quantitative trait loci associated with contents of 16 elements in unmilled rice grain.Theor Appl Genet,2014 127: 137-165. |
| [10] | Du J,Zeng D L,Wang B,Qian Q,Zheng S,Ling H Q.Environmental effects on mineral accumulation in rice grains and identification of ecological specific QTLs.Environ GeoChem Health,2013,35(2):161-170. |
| [11] | Zhang X Q,Zhang G P,Guo L B,Wang H,Zeng D L.Identification of quantitative trait loci for Cd and Zn concentrations of brown rice grown in Cd-polluted soils.Euphytica,2011,180(2):173-179. |
| [12] | Ishikawa S,Abe T,Kuramata M,Yamaguchi M,Ando T,Yamamoto T,Yano M.A major quantitative trait locus for increasing cadmium-specific concentration in rice grain is located on the short arm of chromosome 7.J Exp Bot,2010,61: 923-934. |
| [13] | Xu Q,Zheng T Q,Hu X,Cheng L R,Xu J L,Shi Y M,Li Z K.Examining two sets of introgression lines in rice (Oryza sativa L.) reveals favorable alleles that improve grain Zn and Fe concentrations. PLoS One,2015,10(7):e0131846. doi:10.1371/journal.pone.0131846. |
| [14] | Norton G J,Deacon C M,Xiong L Z,Huang S Y,Meharg A A.Genetic mapping of the rice ionome in leaves and grain: Identification of QTLs for 17 elements including arsenic, cadmium, iron and selenium.Plant Soil,2010,329(1/2):139-153. |
| [15] | Norton G J,Duan G L,Lei M,Zhu Y G,Meharg A A,Price A H.Identification of quantitative trait loci for rice grain element composition on an arsenic impacted soil: Influence of flowering time on genetic loci.Ann Appl Biol,2012,161(1):46-56. |
| [16] | Kumar K,Jian S,Jian R K.Linkage mapping for grain iron and zinc content in F2 population derived from the cross between PAU201 and Palman 579 in rice (Oryza sativa L.). Cereal Res Comm,2014,42(3):389-400. |
| [17] | Lu K Y,Li L Z,Zheng X F,Zhang Z,Mou T,Hu Z.Quantitative trait loci controlling Cu, Ca, Zn, Mn and Fe content in rice grains.J Genet,2008,87(3):305-310. |
| [18] | Yu Y H,Shao Y F,Liu J,Fan Y Y,Sun X C,Cao Z Y,Zhuang J Y.Mapping of quantitative trait loci for contents of macro- and microelements in milled rice (Oryza sativa L.). J Agric Food Chem,2015,63, 7813-7818. |
| [19] | Anuradha K,Agarwal S,Rao K V,Rao K V,Viraktamath B C,Sarla N.Mapping QTLs and candidate genes for iron and zinc concentrations in unpolished rice of Adhukar × Swarna RILs.Gene,2012,508(2):233-240. |
| [20] | Garcia-Oliveira A L,Tan L B,Fu Y C,Sun C Q. Genetic identification of quantitative trait loci for contents of mineral nutrients in rice grain.J Integr Plant Biol,2009,51: 84-92. |
| [21] | 黄莹莹,邹德堂,王敬国,刘化龙,兴旺,马婧,武琦. 水稻子粒锰、铁、锌、铜含量的QTL定位分析. 作物杂志, 2012(6):77-81. |
| [21] | Huang Y Y,Zou D T,Wang J G,Liu H L,Xing W,Ma J,Wu Q.QTL mapping for Mn, Fe, Zn and Cu contents in rice grains.Crops, 2012(6):77-81. (in Chinese with English abstract) |
| [22] | Sands D C,Morris C E,Dratz E A,Pilgeram A.Elevating optimal human nutrition to a central goal of plant breeding and production of plant-based foods.Plant Sci,2009,177(5):377-389. |
| [23] | Anandan A,Rajiv G,Eswaran R,Prakash M.Genotypic variation and relationships between quality traits and trace elements in traditional and improved rice (Oryza sativa L.) genotypes. J Food Sci,2011,76(4):122-130. |
| [24] | Jiang S L,Shi C H,Wu J G.Studies on mineral nutrition and safety of wild rice (Oryza L.). Int J Food Sci Nutr,2009,60(s1):139-147. |
| [25] | Tanksley S D, McCouch S R. Seed banks and molecular maps: Unlocking genetic potential from the wild.Science,1997,277: 1063-1066. |
| [26] | 黄得润,陈洁,侯丽娟,樊叶杨,庄杰云.协青早B//协青早B/东乡野生稻BC1F5群体产量性状QTL分析.农业生物技术学报,2008,16(6):977-982. |
| [26] | Huang D R,Chen J,Hou L J,Fan Y Y,Zhuang J Y.Identification of QTLs for yield traits in the BC1F5 population of Xieqingzao B//Xieqingzao B/Dongxiang wild rice.J Agric Biotech,2008,16(6):977-982. (in Chinese with English abstract) |
| [27] | Zheng K L,Huang N,Bennet J.PCR-based marker-assisted selection in rice breeding//IRRI Discussion Paper Series No. 12. Manila: IRRI,1995: 1-24. |
| [28] | Wang S C,Basten C J,Zeng Z B.Windows QTL cartographer 2.5. Raleigh, NC: Department of Statistics, North Carolina State University, 2012. Windows QTL cartographer 2.5. Raleigh, NC: Department of Statistics, North Carolina State University, 2012. . |
| [29] | McCouch S R, CGSNL. Gene nomenclature system for rice.Rice,2008,1: 72-84. |
| [30] | Jiang S L,Wu J G,Thang N B,Feng Y,Yang X E,Shi C H.Genotypic variation of mineral elements contents in rice (Oryza sativa L.). Eur Food Res Technol,2008,228: 115-122. |
| [31] | Jiang S L,Wu J G,Feng Y,Yang X E,Shi C H.Correlation analysis of mineral element contents and quality traits in milled rice (Oryza sativa L.). J Agric Food Chem,2007,55(23):9608-9613. |
| [32] | 孙明茂,杨仁昌,李点浩,崔仁守,曹桂兰,李圭星,金弘烈,于元杰,李英泰,韩龙植.粳稻“龙锦1号/香软米1578”F3家系群糙米矿质元素含量变异及相关性分析.中国水稻科学,2008,22(3):290-296. |
| [32] | Sun M M,Yang R C,Li D H,Choi I S,Cao G L,Lee K S,Kim H Y,Yu Y J,Lee Y T,Han L Z.Variation and correlation analysis of mineral element contents in brown rice of F3 lines from japonica combination “Longjin 1/Xiangruanmi 1578”.Chin J Rice Sci,2008,22(3):290-296. (in Chinese with English abstract) |
| [33] | 曾亚文,申时全,汪禄祥,刘家富,普晓英,杜娟.云南稻种矿质元素含量与形态及品质性状的关系.中国水稻科学,2005,19(2):127-131 |
| [33] | Zeng Y W,Shen S Q,Wang L X,Liu J F,Pu X Y,Du J.Relationship between morphological and quality traits and mineral element content in Yunnan rice.Chin J Rice Sci,2005,19(2):127-131. (in Chinese with English abstract) |
| [34] | Pinson S R M,Tarpley L,Yan W G,Yeateref K,Lahnerg B,Yakubovah E,Huangi X Y,Zhangj M,Mary G M L,Salti D E. Worldwide genetic diversity for mineral element concentrations in rice grain.Crop Sci,2015,55(1):294-311. |
| [35] | Huang Y,Tong C,Xu F F,Chen Y L,Zhang C Y,Bao J S.Variation in mineral elements in grains of 20 brown rice accessions in two environments.Food Chem,2016,192: 873-878. |
/
| 〈 |
|
〉 |