超级早稻中早39稻瘟病抗性的系谱分析
收稿日期: 2016-09-21
修回日期: 2017-03-23
网络出版日期: 2017-07-10
基金资助
中央级公益性科研院所基本科研业务费资助项目(2014RG001-3)
Genealogical Analysis on Resistance to Magnaporthe oryzae of Super Early Rice Zhongzao 39
Received date: 2016-09-21
Revised date: 2017-03-23
Online published: 2017-07-10
目的研究常规超级早籼稻品种中早39持久高抗稻瘟病的遗传基础,【方法】利用田间抗性鉴定、稻瘟病抗性基因分子标记检测和基因测序的方法,分析中早39稻瘟病持久抗性的遗传基础。结果中早39、金早47和中组3号对7个稻瘟病抗性基因Piz、Pi9、Pib、Pi36、Pi64、Pi-d2和Pi-d3的分子标记均呈阳性条带;且中早39与金早47、中组3号对5个稻瘟病生理小种的抗性表现完全相同。中早39与金早47、中组3号无论是表型还是基因型都完全一致;序列分析结果表明中早39系谱中的供试材料均含有Pi-d2抗病等位基因。结论中早39的抗稻瘟病特性经中组3号,来源于金早47。含有Pi-d2的地谷与中早39、金早47和中组3号对5个稻瘟病生理小种的表型相同。
梁燕, 季芝娟, 曾宇翔, 张翠真, 吴晗霖, 杨长登 . 超级早稻中早39稻瘟病抗性的系谱分析[J]. 中国水稻科学, 2017 , 31(4) : 441 -446 . DOI: 10.16819/j.1001-7216.2017.6165 441
【Objective】 To clarify the genetic basis of the durable resistance to Magnaporthe oryzae of the super early rice Zhongzao 39,【Method】blast resistance assessment, molecular marker screening and gene sequencing were conducted.【Result】Zhongzao 39, Jinzao 47 and Zhongzu 3 showed positive bands to all the molecular markers for 7 resistant genes Piz, Pi9, Pib, Pi36, Pi64, Pi-d2 and Pi-d3; The resistance performance of Zhongzao 39, were fully consistent with Jinzao 47 and Zhongzu 3 to the 5 isolates collected from Zhejiang and Jiangxi Province, China; Molecular sequencing results showed that all the test materials contain Pi-d2 resistant alleles. 【Conclusion】These results showed that the genetic basis of durable resistance to Magnaporthe oryzae of Zhongzao 39 was contributed by Jinzao 47 and Zhongzu 3.
Key words： molecular marker; resistant gene; gene sequencing; genealogical analysis
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