干旱胁迫下杂草稻和栽培稻根系基因表达差异研究
收稿日期: 2015-12-09
修回日期: 2016-01-23
网络出版日期: 2016-09-10
基金资助
黑龙江省农业科学院博士后工作站;哈尔滨市科技局项目(2014RFQYJ125);黑龙江省农业科学院创新工程项目(2014QN006);黑龙江省农业科学院博士人员科研启动金项目(201507-16);国家水稻产业技术体系项目(CARS-01-43);北方寒地粳稻资源研究与新品种选育项目(2014BAD01B03-02)
Global Genome Expression Analysis of Root Genes under Drought Stress in Weedy Rice and Up-land Rice
Received date: 2015-12-09
Revised date: 2016-01-23
Online published: 2016-09-10
利用Affymetrix水稻表达谱芯片(GeneChip Rice Genome Array)研究抗旱杂草稻HEB07-2与巴西陆稻(IAPAR9)在PEG模拟干旱胁迫下根系基因表达变化。结果表明,杂草稻HEB07-2转录组对干旱信号的响应程度与方向均与巴西陆稻存在很大差异,HEB07-2以正向调控为主,而巴西陆稻以负调控为主。干旱胁迫下杂草稻HEB07-2与巴西陆稻分别有6878个和2923个基因表达,其中HEB07-2受干旱胁迫诱导上调的基因有4693个,下调的基因有2185个;而巴西陆稻则分别有983个和1940个。在差异基因表达的倍数上也是HEB07-2高于巴西陆稻。进一步的GO分析表明,在干旱胁迫下,HEB07-2和IAPAR9根系基因响应途径的差异表现为HEB07-2在钾离子转运(GO:0006813)、次生物质代谢(GO:0019748)、细胞生长(GO:0016049)、葡萄糖代谢(GO:0006006)、跨膜离子转运器活性(GO:0015075)、亚铁血红素结合体(GO:0020037)、氧化还原酶活性(GO:0016491)等方面基因显著上调,这与生理数据和表型数据一致。
丁国华, 孙健, 杨光, 张凤鸣, 白良明, 孙世臣, 姜树坤, 王彤彤, 郑洪亮, 夏天舒, 沈希宏, 马殿荣, 陈温福 . 干旱胁迫下杂草稻和栽培稻根系基因表达差异研究[J]. 中国水稻科学, 2016 , 30(5) : 458 -468 . DOI: 10.16819/j.1001-7216.2016.5180
The expression changes of root genes in drought-resistance weedy rice HEB07-2 (Oryza sativa f. spontanea) and up-land rice IAPAR9 (Oryza sativa) were analyzed under polyethylene glycol(PEG)-simulated with drought stress condition with Affymetrix GeneChip rice genome array. The results indicated that the extent and direction of transcriptome response of HEB07-2 and IAPAR9 to drought differed greatly. For HEB07-2, among 6878 expressed genes, 4693 were up-regulated and 2185 were down-regulated under drought condition. For IAPAR9, among 2923 expressed genes,983 were up-regulated and 1940 were down-regulated. Analysis of differentially expressed genes in HEB07-2 and IAPAR9 showed that the weedy rice HEB07-2 had a higher changing fold than the up-land rice IAPAR9. Gene ontology analysis revealed that genes of HEB07-2 in potassium ion transporting(GO: 0006813), secondary metabolite(GO: 0019748), cell growth(GO: 0016049), glucose metabolism(GO:0006006), transmembrane ion transporter activity(GO:0015075), ferroheme coalition(GO:0020037), oxidordeuctase activity(GO:0016491)were significantly up-regulated in comparing with IAPAR9. These were consistent with the physiological and phenotypic data.
Key words： weedy rice; root; gene expression profiling; drought resistance
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