水稻转录因子基因OsPHR3在磷素利用过程中的作用
收稿日期: 2016-01-22
修回日期: 2016-02-04
网络出版日期: 2016-07-10
基金资助
国家自然科学基金资助项目（31172014）；江苏省自然科学基金资助项目（BK20141367）；转基因生物新品种培育科技重大专项（2014ZX0800931B; 2016ZX08009003005）。
Roles of Transcription Factor Gene OsPHR3 on the Utilization of Phosphate in Rice
Received date: 2016-01-22
Revised date: 2016-02-04
Online published: 2016-07-10
磷是植物体生长发育所必需的大量营养元素之一，广泛参与植物体多种生命活动。土壤中磷的有效性很低，是农业生产中限制作物产量的重要因素。OsPHR3（LOC_Os02g04640）属于MYBCC家族，与水稻中磷信号途径中心调控因子OsPHR2是同源基因,且具有部分功能重叠。本研究利用转基因技术获得OsPHR3基因的突变体和超表达材料，通过水培实验、32Pi同位素实验以及桶培实验来研究该基因在吸收利用磷素过程中的作用。水培实验表明，与野生型相比，突变体磷含量无明显差异，基因超表达能够提高水稻体内磷含量。32Pi同位素实验显示，与野生型相比，缺磷时突变体吸收速率降低，而该基因超表达能够促进磷素的吸收与转运。桶培实验表明，该基因超表达能够增加水稻有效分蘖数，提高种子中磷含量，该基因缺失使得穗长变短。OsPHR3基因可能调控促进磷的吸收与向地上部转运。该研究将为以后分子育种提供依据。
张亮,孙文献,孙雅菲,裴文霞,罗闻真,孙瑞,张占田,徐国华,孙淑斌* . 水稻转录因子基因OsPHR3在磷素利用过程中的作用[J]. 中国水稻科学, 2016 , 30(4) : 397 -405 . DOI: 10.16819/j.1001-7216.2016.6011
Phosphorus, an essential macronutrient element for plant growth and development, is broadly participating in various plant life activities. However, low availability of soil phosphate is a major constraint for crop production in many agricultural systems. OsPHR3（LOC_Os02g04640）is a homolog of OsPHR2, the central regulator of Pisignaling in rice. Both OsPHR2and OsPHR3 belong to the MYBCC family members and have functional redundancy in part. In this study, OsPHR3 knockout and overexpression transgenic plants were obtained. The hydroponic culture, 32Pi isotopic and pot experiments were used to demonstrate the role of OsPHR3 in rice phosphate untilization. First, hydroponic experiments showed that overexpression of OsPHR3increased the phosphorus content of the rice, although the knockout lines had no significant difference with wild type plants. Second, 32Pi isotopic experiments revealed that overexpression of the gene promoted the absorption and distribution of phosphate, while the absorption rate in knockout lines decreased in comparison with wild type plants under Pideficient condition. Third, pot experiments demonstrated that overexpression of OsPHR3 enhanced the effective tillers and phosphorus content in seed. However, the panicle length in knockout lines is shorter than in wild type plants. In conclusion, OsPHR3 is a potential transcription factor for improving P efficiency in rice through molecular breeding.
[1]胡培松, 翟虎渠. 中国水稻生产新特点与稻米品质改良. 中国农业科技导报, 2002, 4(4):3339.
Hu P S,Zhai H Q.New characteristics of rice production and quality improvement in China.Rev China Agric sci Techhol,2002,4(4):3339.(in Chinese with English abstract).
[2]彭少兵，黄见良，钟旭华，等.提高中国稻田氮肥利用率的研究策略.中国农业科学, 2002, 35(9):10951103.
Peng S B, Huang J L,Zhang X H,et al.Research strategy in improving fertilizernitrogen use efficiency of irrigated rice in China,Sci Agric Sin, 2002,35(9):10951103.(in Chinese with English abstract).
[3]Vance C P, UhdeStone C, Allan D L. Phosphorus acquisition and use: Critical adaptations by plants for securing a nonrenewable resource. New Phytol, 2003, 157: 423447.
[4]Abel S, Ticconi C A, Delatorre C A. Phosphate sensing in higher plants. Physiol Plant, 2002, 115(1): 18.
[5]Bieleski R L. Phosphate pools, phosphate transport, and phosphate availability. Ann Rev Plant Physiol, 1973, 24(1): 225252.
[6]Péret B, Clément M, Nussaume L, et al. Root developmental adaptation to phosphate starvation: Better safe than sorry. Trends Plant Sci, 2011, 16(8): 442450.
[7]Bonfante P, Genre A. Mechanisms underlying beneficial plantfungus interactions in mycorrhizal symbiosis. Nat Commun, 2010, 1: 48.
[8]Hinsinger P. Bioavailability of soil inorganic P in the rhizosphere as affected by rootinduced chemical changes: A review. Plant Soil, 2001, 237(2): 173195.
[9]LópezArredondo D L, HerreraEstrella L. Engineering phosphorus metabolism in plants to produce a dual fertilization and weed control system. Nat Biotechnol, 2012, 30(9): 889893.
[10]Rubio V, Linhares F, Solano R, et al. A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae. Genes & Devel, 2001, 15: 21222133.
[11]Bustos R, Castrillo G, Linhares F, et al. A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis. PLoS Genet, 2010, 6(9): e1001102.
[12]Wu P, Wang X M. Role of OsPHR2 on phosphorus homeostasis and root hairs development in rice.Plant Signal & Behav, 2008, 3(9): 674675.
[13]Zhou J, Jiao F C, Wu Z C, et al. OsPHR2 is involved in phosphatestarvation signaling and excessive phosphate accumulation in shoots of plants. Plant Physiol, 2008, 146(4)16731686.
[14]Guo M N, Ruan W Y, Li C Y, et al. Integrative comparison of the role of the PHR1 subfamily in phosphate signaling and homeostasis in rice. Plant Physiol, 2015, 168(4):176276.
[15]Aung K, Lin S I, Wu C C, et al. Pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene. Plant Physiol, 2006, 141: 10001011.
[16]Bari R, Pant B D, Stitt M, et al. PHO2, microRNA399, and PHR1 define a phosphatesignaling pathway in Plants. Plant Physiol, 2006, 141: 988999.
[17]González E, Solano R, Rubio V, et al. Phosphate transporter traffic facilitator1 is a plantspecific SEC12related protein that enables the endoplasmic reticulum exit of a highaffinity phosphate transporter in Arabidopsis. Plant Cell, 2005, 17: 35003512.
[18]Schachtman D P, Shin R. Nutrient sensing and signaling: NPKS. Annu Rev Plant Biol, 2007, 58: 4769.
[19]Nilsson L, Muller R, Nielsen T H. Increased expression of the MYBrelated transcription factor, PHR1, leads to enhanced phosphate uptake in Arabidopsis thaliana. Plant Cell Environ, 2007, 30: 14991512.
[20]Chiou T J, Lin S I. Signaling network in sensing phosphate availability in plants. Anu Rev Plant Biol, 2011, 62:185206.
[21]Wu P, Shou H X, Xu G H, et al. Improvement of phosphorus affinity in rice on the basis of understanding phosphate signaling and homeostasis. Curr Opin Plant Biol, 2013, 16:18.
[22]Liu F, Wang Z Y, Ren H Y, et al. OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice. Plant J, 2010, 62: 508517.
[23]Bayle V, Arrighi J F, Creff A, et al. Arabidopsis thaliana highaffinity phosphate transporters exhibit multiple levels of posttranslational regulation. Plant Cell, 2011, 23:1523.
[24]Shin H, Shin H S, Dewbre G R, Harrison M J.Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low and highphosphate environments. Plant J, 2004, 39: 629642.
[25]Khan G A, Bouraine S, Wege S, et al. Coordination between zinc and phosphate homeostasis involves the transcription factor PHR1, the phosphate exporter PHO1, and its homologue PHO1;H3 in Arabidopsis. J Exp Bot, 2014, 65: 871884.
[26]Chen J Y, Liu Y, Ni J, et al. OsPHF1 regulates the plasma membrane localization of low and highaffinity inorganic phosphate transporters and determines inorganic phosphate uptake and translocation in Rice. Plant Physiol, 2011, 157: 269278.
[27]Bang X J, Finnegan. Regulation of phosphate starvation responses in higher plants. Ann Bot (Lond) PM, 2010, 105: 513526.
[28]FrancoZorrilla J M, González E, Bustos R, et al. The transcriptional control of plant responses to phosphate limitation. J Exp Bot, 2004, 55: 285293.
[29]Yang X J. Finnegan Regulation of phosphate starvation responses in higher plants. Ann Bot (Lond) PM, 2010, 105: 513526.
[30]Ren F, Guo Q Q, Chang L L, et al. Brassicanapus PHR1 gene encoding a MYBlike protein functions in response to phosphate starvation. PLoS ONE, 2012, 7: e44005.
[31]Wu P, Xu J I. Does OsPHR2, central Pisignaling regulator, regulate some unknown factors crucial for plant growth? Plant Signal & Beh, 2010, 5:6, 712714.
[32]Wissuwa M. How do plants achieve tolerance to phosphorus deficiency? Small causes with big effects. Plant Physiol, 2003, 133:19471958.
[33]Yi K K, Wu Z C, Zhou J, et al. OsPTF1, a novel transcription factor involved in tolerance to phosphate starvation in rice. Plant Physiol, 2005, 138:20872096.
[34]Wang Z Y, Ruan W Y, Shi J, et al. Rice SPX1 and SPX2 inhibit phosphate starvation responses through interacting with PHR2 in a phosphatedependent manner. Proc Natl Acad Sci, 2014, 11: 1495314958.
/
| 〈 |
|
〉 |