水稻类病斑突变体wy3的鉴定和基因定位
# 共同第一作者;
收稿日期: 2015-11-30
修回日期: 2015-12-24
网络出版日期: 2016-05-10
基金资助
国家自然科学基金资助项目(31071384,31000533);江苏省科技支撑计划资助项目(BE2013301);江苏高校优势学科建设工程资助项目
Characterization and Gene Mapping of Lesion Mimic Mutant wy3 in Rice
# These authors contributed equally to this work;
Received date: 2015-11-30
Revised date: 2015-12-24
Online published: 2016-05-10
在粳稻品种武育粳3号栽培群体中获得一个类病斑突变体wy3。该突变体类病斑出现于苗期,分蘖期扩散至整张叶片,属于扩散型类病斑突变体。相比野生型,突变体wy3的株高明显降低,有效分蘖数减少,穗长、每穗粒数、结实率均显著降低。遮光处理表明,突变体wy3类病斑的产生受自然光诱导。台盼蓝染色结果表明,类病斑部位有大量的死亡细胞。突变体wy3的光合色素含量和净光合速率较野生型显著降低,SOD、POD、CAT活性和MDA含量均显著高于野生型。遗传分析表明突变体表型受单隐性核基因控制,采用BSA将该基因初步定位在第2染色体短臂端粒附近。采用F2群体中1099株类病斑单株将基因定位在标记W2-17和W2-18之间28kb的物理距离内。测序结果表明,突变体wy3中的LOC_Os02g02000编码区(CDS)第375位碱基C缺失,导致翻译提前终止,突变体中该候选基因为OsHPL3的一个新等位基因。
张宏根, 王茂宇, 张丽佳, 胡雅, 马佳琦, 张翼帆, 汤述翥, 梁国华, 顾铭洪 . 水稻类病斑突变体wy3的鉴定和基因定位[J]. 中国水稻科学, 2016 , 30(3) : 239 -246 . DOI: 10.16819/j.1001-7216.2016.5176
A lesion mimic mutant wy3 was obtained from the progeny of a japonica variety Wuyujing 3 by natural mutation. The lesions were first observed on the leaves at the seedling stage, then spread gradually to the whole leaf at the initial tillering stage. Compared with the wild type(WT), the plant height, the number of effective tiller, panicle length, grain number per panicle and seed setting rate of wy3 were reduced significantly. The shading assay showed that the lesions on leaves of wy3 were induced by natural light. Compared with the wild type, the photosynthetic pigment and the net photosynthetic rate of wy3 were significantly reduced, but the SOD activity, POD activity, CAT activity and MDA content of wy3 were significantly higher than those of WT. Trypan blue staining experiments showed that there were a large number of dead cells in the mutant lesion. Genetic analysis suggested that the phenotype of wy3 was controlled by a single recessive nuclear locus, and the target gene was mapped between markers W2-17 and W2-18 with the physical distance of 28 kb on chromosome 2. Sequence analysis revealed that the mutated gene in wy3 had a single nucleotide deletion at the 375th in CDS of LOC_Os02g02000, resulting in a premature termination of translation of the target gene, which is an new allele of OsHPL3.
Key words： rice; lesion mimic mutant; gene mapping
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