水稻迟抽穗突变体dth9的遗传分析与基因定位
# 共同第一作者;
收稿日期: 2015-10-26
修回日期: 2015-12-19
网络出版日期: 2016-05-10
基金资助
国家自然科学基金资助项目(31501279,31521064,31271700);国家973计划资助项目(2013CBA01405);中国博士后科学基金资助项目(2015M570181);深圳市科技计划资助项目(JCYJ20150630165133402)
Genetic Analysis and Gene Mapping of a Heading-delayed Mutant dth9 in Rice (Oryza sativa L.)
# These authors contributed equally to this work;
Received date: 2015-10-26
Revised date: 2015-12-19
Online published: 2016-05-10
叶卫军, 胡时开, 吴立文, 郭龙彪, 钱前 . 水稻迟抽穗突变体dth9的遗传分析与基因定位[J]. 中国水稻科学, 2016 , 30(3) : 232 -238 . DOI: 10.16819/j.1001-7216.2016.5156
A heading-delayed mutant, dth9 (days to heading 9)was identified from an ethyl methylsulfonate (EMS)-induced 93-11 mutant library. The heading date of dth9 delayed about 50 days compared to the wild-type and there was no significant difference in other agronomic traits. Genetic analysis showed that the phenotype of dth9 was controlled by a single recessive nuclear gene. To map this gene, two F2 populations were generated by crossing the dth9 mutant with Nipponbare or Wuyunjing 7 as mapping populations. By using SSR markers and eight new designed InDel markers, DTH9 was narrowed to a 240kb interval between the markers D9-9 and D9-17 near the centromere of chromosome 9, there were no reports about genes associated with heading date in this interval. In addition, the expression levels of genes related to heading date were significantly decreased in dth9 by quantitative real-time PCR analysis.
Key words： rice; heading date; genetic analysis; gene mapping
| [1] | 刘广林, 罗群昌, 陈远孟, 等. 水稻种质资源抽穗扬花期耐冷性鉴定评价. 西南农业学报, 2013, 26(2): 395-398. |
| [1] | Liu G L, Luo Q C, Chen Y M, et al.Analysis and evaluation on cold tolerance for rice germplasm resources at flowering stage.Southwest China J Agric Sci, 2013, 26(2): 395-398.(in Chinese with English abstract) |
| [2] | 邱磊, 蒋海潮, 冯玉涛, 等.控制水稻抽穗期和株高的QTL定位及遗传分析. 基因组学与应用生物学, 2014, 33(4): 828-835 |
| [2] | Qiu L, Jiang H C,Feng Y T, et al.Mapping and genetic analycis of QTL for heading date and plant height in rice.Genonm Appl Biol,2014,33(4):828-835.(in Chinese with English abstract) |
| [3] | 邓晓建, 周开达, 李仁端, 等. 水稻完全显性早熟性的发现和基因定位. 中国农业科学, 2001, 34(3): 233-239. |
| [3] | Deng X J, Zhou K D, Li R D, et al.Identification and gene mapping of completely dominant earliness in rice (Oryza sativa L.).Sci Agric Sin, 2001, 34(3): 233-239.(in Chinese with English abstract) |
| [4] | 胡时开, 苏岩, 叶卫军, 等. 水稻抽穗期遗传与分子调控机理研究进展. 中国水稻科学, 2012, 26(3): 373-382. |
| [4] | Hu S K, Sun Y, Ye W J, et al.Advances in genetic analysis and molecular regulation mechanism of heading date in rice (Oryza sativa L.).Chin J Rice Sci, 2012, 26(3): 373-382.(in Chinese with English abstract) |
| [5] | Yano M, Katayose Y, Ashikari M, et al.Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS,Plant Cell, 2000, 12(12): 2473-2484. |
| [6] | Kojima S, Takahashi Y, Kobayashi Y, et al.Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day conditions.Plant Cell Physiol, 2002, 43(10): 1096-1105. |
| [7] | Doi K, Izawa T, Fuse T, et al.Ehd1, a B-type response regulator in rice, confers short-day promotion of flowering and controls FT-like gene expression independently of Hd1.Genes & Dev, 2004, 18(8): 926-936. |
| [8] | Takahashi Y, Shomura A, Sasaki T, et al.Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the alpha subunit of protein kinase CK2.Proc Natl Acad Sci USA, 2001, 98(14): 7922-7927. |
| [9] | Matsubara K, Yamanouchi U, Nonoue Y, et al.Ehd3, encoding a plant homeodomain finger-containing protein, is a critical promoter of rice flowering.Plant J, 2011, 66(4): 603-612. |
| [10] | Gao H, Zheng X M, Fei G, et al.Ehd4 encodes a novel and Oryza-genus-specific regulator of photoperiodic flowering in rice.Plos Genet, 2013, 9(2): e1003281. |
| [11] | Xue W, Xing Y, Weng X, et al.Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice.Nat Genet, 2008, 40(6): 761-767. |
| [12] | Wei X, Xu J, Guo H, et al.DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously.Plant Physiol, 2010, 153(4): 1747-1758. |
| [13] | Wu K S, Tanksley S D.Abundance, polymorphism and genetic mapping of microsatellites in rice.Mol Gen Genet, 1993, 241(1): 225-235. |
| [14] | Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method.Methods, 2001, 25: 402-408. |
| [15] | Hirochika H, Guiderdoni E, An G, et al.Rice mutant resources for gene discovery.Plant Mol Biol, 2004, 54(3): 325-334. |
| [16] | 郭梁, 张振华, 庄杰云. 水稻抽穗期QTL及其与产量性状遗传控制的关系. 中国水稻科学, 2012, 26(2): 235-245. |
| [16] | Guo L, Zhang Z H, Zhuang J Y.Quantiative trait loci for heading date and their relationship with the genetic control of yield traits in rice (Oryza sativa).Chin J Rice Sci, 2012, 26(2): 235-245.(in Chinese with English abstract) |
| [17] | Thomson M J, Edwards J D, Septiningsih E M, et a1. Substitution mapping of dth1.1, a flowering-time quantitative trait locus (QTL) associated with transgressive variation in rice, reveals multiple sub-QTL.Genetics, 2006, 172: 2501-2514. |
| [18] | Lin H, Ashikari M, Yamanouchi U, et a1. Identification and characterization of a quantitative trait locus, Hd9, controlling heading date in rice.Breeding Sci, 2002, 52(1): 35-41. |
| [19] | Takeuchi Y, Lin S Y, Sasaki T, et a1. Fine linkage mapping enables dissection of closely linked quantitative trait loci for seed dormancy and heading in rice.Theor Appl Genet, 2003, 107(7): 1174-1180. |
| [20] | Matsubara K, Kono I, Hori K, et a1. Novel QTLs for photoperiodic flowering revealed by using reciprocal backcross inbred lines from crosses between japonica rice cultivars.Theor Appl Genet, 2008, 117(6): 935-945. |
| [21] | Monna L, Lin H X, Kojima S, et a1. Genetic dissection of a genomic region for a quantitative trait locus, Hd3, into two loci, Hd3a and Hd3b, controlling heading date in rice.Theor Appl Genet, 2002, 104(5): 772-778. |
| [22] | Lin H, Liang Z W, Sasaki T, et a1. Fine mapping and characterization of quantitative trait loci Hd4 and Hd5 controlling heading date in rice.Breeding Sci, 2003, 53(1): 51-59. |
| [23] | Yamamoto T, Kuboki Y, Lin S Y, et a1. Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors.Theor Appl Genet, 1998, 97(1): 37-44. |
| [24] | Yan W H, Wang P, Chen H X, et al.A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice.Mol Plant, 2011, 4(2): 319-330. |
| [25] | 杜雪树, 戚华雄, 廖世勇, 等.水稻抽穗期分子生物学研究进展. 湖北农业科学, 2013,52(24): 5958-5962. |
| [25] | Du X S,Qi H X,Liao S Y, et al.Advances on the molecular biology of rice heading date.Hubei Agric Sci,2013,52(24):5958-5962.(in Chinese) |
/
| 〈 |
|
〉 |