水稻WRKY转录因子基因家族响应外源一氧化氮的表达谱分析
收稿日期: 2015-09-16
修回日期: 2015-11-30
网络出版日期: 2016-03-10
基金资助
国家自然科学基金资助项目(31171803, 31301617);湖南省教育厅一般资助项目(14C0453);湖南科技大学创新基金资助项目(S140031)
Expression Profiles of Rice WRKY Transcription Factor Gene Family Responsive to Exogenous Nitric Oxide Application
Received date: 2015-09-16
Revised date: 2015-11-30
Online published: 2016-03-10
一氧化氮(nitric oxide, NO)信号分子与WRKY转录因子均参与植物抗逆、发育与代谢等许多生理过程。采用Agilent水稻全基因组cDNA芯片分析了NO处理后1、6和12 h水稻幼苗WRKY转录因子基因的表达谱,鉴定出在1个时间点有两倍或两倍以上表达变化的WRKY基因32个,主要分布在WRKY的Ⅰ和Ⅱ组,其中75%的Ⅱa和45.6%的Ⅱd亚组成员为差异表达基因;鉴定出至少在2个时间点有两倍或两倍以上表达变化的WRKY基因15个,均为早期(1 h)应答,且多数(64.2%)持续上调;基因功能预测分析表明,这些基因主要参与生物学过程中的细胞过程、代谢过程和刺激响应,以及分子功能中的转录调节活性和结合;代谢通路分析表明,WRKY24涉及植物与病原菌相互作用代谢通路。实时荧光定量PCR 验证结果与芯片杂交结果基本一致,印证了芯片杂交结果的有效性。上述发现提示,NO信号可能参与了WRKY转录因子介导的生物学调控功能,并为这些基因的进一步功能分析奠定基础。
孟姣, 王海华, 向建华, 蒋丹, 彭喜旭, 贺欢欢 . 水稻WRKY转录因子基因家族响应外源一氧化氮的表达谱分析[J]. 中国水稻科学, 2016 , 30(2) : 111 -120 . DOI: 10.16819/j.1001-7216.2016.5138
Signal molecule nitric oxide (NO) and WRKY transcription factors are involved in many physiological processes, such as defense against stresses, development and metabolism in plants. In the present study, we investigated the expression patterns of WRKY transcription factor gene family in rice seedlings at 1, 6 and 12 after exogenous NO treatments using Agilent rice cDNA oligo microarray. Totally, 32 differently expressed WRKY genes, whose expression levels increased or decreased by at least two-fold at one time point compared with the control (T0), were identified. These WRKY genes were mainly distributed over Groups Ⅰ and Ⅱ, among which 75% of Ⅱa and 45.6% of Ⅱd subgroup members were differently expressed upon the NO treatments. Moreover, 15 identified NO-responsive WRKY genes, whose expression level changed by more than two-fold at least two time points among the three time points compared with the control, exhibited an earlier (1 h) response to the NO treatments, and most of them (64.2%) were continually up-regulated. Prediction of gene function revealed that the NO-responsive WRKY genes were mainly involved in cellular process, metabolic process and response to stimulus of biological process, and transcription regulator activity and binding of molecular function. The analysis of metabolic pathways showed that WRKY24 was involved in plant-pathogen interaction pathway. The results of microarray hybridization were largely consistent with those of quantitative real-time PCR, verifying the validity of microarray hybridization. These findings suggest that NO signaling might be involved in the regulatory functions of WRKY transcription factors, and provide a basis for further functional research for these differentially expressed WRKY genes.
| [1] | Riaño-Pachón D M, Ruzicic S, Dreyer I, et al. PlnTFDB: An integrative plant transcription factor database.BMC Bioinform, 2007, 8: 42. |
| [2] | Eulgem T, Rushton P J, Robatzek S, et al.The WRKY superfamily of plant transcription factors.Trends Plant Sci, 2000, 5: 199-206. |
| [3] | Pandey S P, Somssich I E.The role of WRKY transcription factors in plant immunity.Plant Physiol, 2009, 150: 1648-1655. |
| [4] | Agarwal P, Reddy M P, Chikara J.WRKY: Its structure, evolutionary relationship, DNA-binding selectivity, role in stress tolerance and development of plants.Mol Biol Rep, 2011, 38: 3883-3896. |
| [5] | Chen L, Song Y, Li S, et al.The role of WRKY transcription factors in plant abiotic stresses.Biochim Biophys Acta, 2012, 1819: 120-128. |
| [6] | Li J, Brader G, Palva E T.The WRKY70 transcription factor: A node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense. Plant Cell, 2004, 16: 319-331. |
| [7] | Qiu D, Xiao J, Ding X, et al.OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate- and jasmonate-dependent signaling.Mol Plant Microbe Interact, 2007, 20: 492-499. |
| [8] | Wang H, Meng J, Peng X, et al.Rice WRKY4 acts as a transcriptional activator mediating defense responses toward Rhizoctonia solani, the causing agent of rice sheath blight.Plant Mol Biol, 2005, 89(1/2): 157-171. |
| [9] | Shang Y, Yan L, Liu Z Q, et al.The Mg-chelatase H subunit of Arabidopsis antagonizes a group of WRKY transcription repressors to relieve ABA-responsive genes of inhibition.Plant Cell, 2010, 22, 1909-1935. |
| [10] | Ryu H S, Han M, Lee S K, et al.A comprehensive expression analysis of the WRKY gene superfamily in rice plants during defense response.Plant Cell Rep, 2006, 25: 836-847. |
| [11] | Ramamoorthy R, Jiang S Y, Kumar N, et al.A comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments.Plant Cell Physiol, 2008, 49: 865-879. |
| [12] | Lamotte O, Courtois C, Pugin L B A, et al. Nitric oxide in plants: The biosynthesis and cell signalling properties of a fascinating molecule.Planta, 2005, 221: 1-4. |
| [13] | Gould K, Lamotte O, Klinguer A, et al.Nitric oxide production in tobacco leaf cells: A generalized stress response?Plant Cell Environ, 2003, 26: 1851-1862. |
| [14] | Delledonne M.NO news is good news for plants.Curr Opin Plant Biol, 2005, 8: 390-396. |
| [15] | Palmieri M C, Sell S, Huang X, et al.Nitric oxide-responsive genes and promoters in Arabidopsis thaliana: A bioinformatics approach.J Exp Bot, 2008, 59:177-186. |
| [16] | Mukhtar M S, Deslandes L, Auriac M-C, et al.The Arabidopsis transcription factor WRKY27 influences wilt disease symptom development caused by Ralstonia solanacearum.Plant J, 2008, 56: 935-947. |
| [17] | Arnon D I.Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris. Plant Physiol, 1949, 24:1-15. |
| [18] | Rice WRKY Working Group. Nomenclature report on rice WRKY's-Conflict regarding gene names and its solution.Rice, 2012, 5: 3. |
| [19] | Wu K L, Guo Z J, Wang H H, et al.The WRKY family of transcription factors in rice and Arabidopsis and their origins.DNA Res, 2005, 12: 9-26. |
| [20] | Dong J, Chen C, Chen Z.Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response.Plant Mol Biol, 2003, 51: 21-37. |
| [21] | Kalde M, Barth M, Somssich I E, et al.Members of the Arabidopsis WRKY group Ⅲ transcription factors are part of different plant defense signaling pathways.Mol Plant Microbe Interact, 2003, 16: 295-305. |
| [22] | Chujo T, Miyamoto K, Shimogawa T, et al.OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate immune responses in rice against rice blast fungus.Plant Mol Biol, 2013, 82: 23-37. |
| [23] | Peng Y, Bartley L E, Chen X, et al.OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice.Mol Plant, 2008, 1: 446-458. |
| [24] | Yokotani N, Sato Y, Tanabe S, et al.WRKY76 is a rice transcriptional repressor playing opposite roles in blast disease resistance and cold stress tolerance.J Exp Bot, 2013, 64(16): 5085-5097. |
| [25] | Liu X, Bai X, Wang X, et al.OsWRKY71, a rice transcription factor, is involved in rice defense response.J Plant Physiol, 2007, 164: 969-979. |
| [26] | Park C Y, Lee J H, Yoo J H, et al.WRKY group IId transcription factors interact with calmodulin.FEBS Lett, 2005, 579: 1545-1550. |
| [27] | Durner J, Wendehenne D, Klessig D F.Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose.Proc Natl Acad Sci USA, 1998, 95: 10328-10333. |
| [28] | Liu X Q, Bai X Q, Qian Q, et al.OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1.Cell Res, 2005, 15(8): 593-603. |
| [29] | Jing S, Zhou X, Song Y, et al.Heterologous expression of OsWRKY23 gene enhances pathogen defense and dark-induced leaf senescence in Arabidopsis. Plant Growth Regul, 2009, 58:181-190. |
| [30] | Song Y, Jing S, Yu D.Overexpression of the stress-induced OsWRKY08 improves osmotic stress tolerance inArabidopsis. Chin Sci Bull, 2009, 54: 4671-4678. |
| [31] | Cheng H, Liu H, Deng Y, et al.The WRKY45-2 WRKY13 WRKY42 transcriptional regulatory cascade is required for rice resistance to fungal pathogen.Plant Physiol, 2015, 167(3): 1087-1099. |
| [32] | Zhang Z L, Shin M, Zou X, et al.A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells.Plant Mol Biol, 2009, 70: 139-151. |
| [33] | Sperotto R A, Boff T, Duarte G L, et al.Increased senescence-associated gene expression and lipid peroxidation induced by iron deficiency in rice roots.Plant Cell Rep, 2008, 27: 183-195. |
| [34] | 黄盼盼. 18个水稻OsWRKY基因的克隆和OsWRKY21功能分析. 广州: 中山大学, 2010. |
| [34] | Huang P P.Cloning 18 OsWRKY genes and functional analysis of OsWRKY21. GuangZhou:Sun Yat-sen University, 2010. (in Chinese with English abstract) |
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