*通讯录作者：E-mail:shiyy123@163.comwnagwensheng02@caas.cn
收稿日期: 2015-01-22
修回日期: 2015-03-28
网络出版日期: 2015-09-10
基金资助
比尔和梅琳盖茨基金资助项目(OPPGD1393)NSFCG云南联合基金资助项目(U1302264)
Development and Application of a Functional Marker for the Cold Stress Responsive Gene OsSADMC
*Corresponding author：E-mailshiyy123@163.com，wnagwensheng02@caas.cn
Received date: 2015-01-22
Revised date: 2015-03-28
Online published: 2015-09-10
通过对95份水稻种质资源进行苗期耐冷性鉴定,筛选出10份苗期耐冷(存活率≥60%)和9份冷敏感的材料(存活率为零),并对水稻冷胁迫响应基因OsSADMC进行了表达分析,发现对照和冷胁迫处理条件下该基因在耐冷品种中的表达量都显著高于冷敏感品种。对耐冷材料丽江新团黑谷(LTH)和冷敏感材料IR29的基因编码区域进行了克隆和测序分析,在两个品种间鉴定了10个SNP位点,其中第749碱基处存在的一个碱基变异(C突变为T),从而使丝氨酸变为亮氨酸。根据其中一个SNP位点设计了OsSADMC的功能标记SADMC-CAPS1,该标记可以准确地鉴定出19份耐冷性不同水稻种质资源OsSADMC的基因型和耐冷性。
胡晓晨, 张婷, 杨圣, 秦巧, 石英尧, 王文生, 傅彬英 . 水稻冷胁迫响应基因OsSADMC功能标记的开发和利用[J]. 中国水稻科学, 2015 , 29(5) : 475 -480 . DOI: 10.3969/j.issn.1001G7216.2015.05.004
Cold tolerance of 95 rice germplasms were screened at seedling stage. Among them, ten cold tolerant lines (survival rate ≥60%) and nine cold sensitive lines (survival rate=0%) were identified. Gene expression analysis of cold stress responsive gene OsSADMC showed that the expression levels of OsSADMC were significantly higher in the cold tolerant lines than those in the cold sensitive lines at both normal and cold stress conditions. Ten SNP loci were identified in the gene coding region between Lijiangxintuanheigu(LTH) and IR29. One amino acid mutation (from serine to leucine) was found between LTH and IR29. A functional marker (SADMC-CAPS1) was developed based on one SNP locus. The allele of OsSADMC carried by ten cold tolerant lines could be distinctly distinguished from that of nine cold sensitive lines by this functional marker.
Key words： rice; cold tolerance; functional marker; OsSADMC
| [1] | 张婷. 长雄野生稻地下茎及耐冷性状功能基因组学及比较转录组学分析[学位论文]. 湖北:武汉大学,2013. |
| [2] | 饶玉春, 杨窑龙, 黄李超, 等. 水稻耐冷胁迫的研究进展. 分子植物育种, 2013, 11(3): 443-450. |
| [3] | 赵明辉, 张文忠, 马殿荣, 等. 水稻耐冷基因研究进展. 沈阳农业大学学报, 2012, 43(6): 711-718. |
| [4] | Zhang Z H, Su L, Li W, et al.A major QTL conferring cold tolerance at the early seedling stage using recombinant inbred lines of rice (Oryza sativa L.).Plant Sci, 2005, 168(2): 527-534. |
| [5] | Ji S L, Jiang L, Wu H, et al.Quantitative trait loci mapping and stability for low temperature germination ability of rice.Plant Breed, 2009, 128: 387-392. |
| [6] | Iwata N, Fujino K.Genetic effects of major QTLs controlling low-temperature germinability in different genetic backgrounds in rice (Oryza sativa L.).Genome, 2010, 53:763-768. |
| [7] | 严长杰, 李欣, 程祝宽, 等. 利用分子标记定位水稻芽期耐冷性基因. 中国水稻科学,1999, 13(3): 134-138. |
| [8] | 钱前, 曾大力, 何平, 等. 水稻籼粳交DH 群体苗期的耐冷性QTLs分析. 科学通报, 1999, 44(22): 2402-2405. |
| [9] | 陈大洲, 钟平安, 肖叶青, 等. 利用SSR 标记定位东乡野生稻苗期耐冷性基因. 江西农业大学学报:自然科学版, 2002, 24(6): 753-757. |
| [10] | 詹庆才, 曾曙珍, 熊伏星, 等. 水稻苗期耐冷性QTLs 的分子定位. 湖南农业大学学报:自然科学版, 2003, 29(1): 7-11. |
| [11] | 韩龙植,乔永利,曹桂兰, 等. 水稻生长早期耐冷性QTL分析.中国水稻科学, 2005, 19(2): 122-126. |
| [12] | Koseki M, Kitazawa N, Yonebayahsi S, et al.Identification and fine mapping of a major quantitative trait locus originating from wild rice, controlling cold tolerance at the seedling stage.Mol Genet Genom, 2010, 284: 45-54. |
| [13] | Dubouzet J G, Sakuma Y, Ito Y, et al.OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt-and cold-responsive gene expression.Plant J, 2003, 33: 751-763. |
| [14] | Su C F, Wang Y C, Hsieh T H, et al.A novel MYBS3-dependent pathway confers cold tolerance in rice.Plant Physiol,2010, 153: 145-158. |
| [15] | Xiang Y, Huang Y, Xiong L.Characterization of stress-responsive CIPK genes in rice for stress tolerance improvement.Plant Physiol, 2007, 144(3): 1416-1428. |
| [16] | Andersen J R, Lübberstedt T.Functional markers in plants.Trends Plant Sci,2003, 8(11): 554-560. |
| [17] | 宋伟, 王凤格, 易红梅, 等. 功能标记及在品种鉴定和辅助育种中的应用前景. 分子植物育种, 2009, 7(3): 612-618. |
| [18] | Alcázar R, Marco F, Cuevas J C, et al.Involvement of polyamines in plant response to abiotic stress.Biotechnol Lett, 2006, 28: 1867-1876. |
| [19] | Kusano T, Berberich T, Tateda C, et al.Polyamines: essential factors for growth and survival.Planta, 2008, 228:367-381. |
| [20] | Chen M, Chen J J, Fang J Y, et al.Down-regulation of S-adenosylmethionine decarboxylase genes results in reduced plant length, pollen viability, and abiotic stresstolerance.Plant Cell Tiss Organ Cult,2014, 116: 311-322. |
| [21] | Roy M, Wu R.Overexpression of S-adenosylmethionine decarboxylase gene in rice increases polyamine level and enhances sodium chloride-stress tolerance.Plant Sci,2002, 163: 987-992. |
| [22] | 耿卫东, 李艳军, 张新宇, 等. 棉花S-腺苷甲硫氨酸脱羧酶基因的克隆及低温下的表达分析. 作物学报, 2012, 38(9): 1649-1656. |
| [23] | Yoshida R, Kanno A, Sato T.Cool-temperature-induced chlorosis in rice plants.Plant Physiol,1996, 110(3): 997-1005. |
| [24] | 徐孟良, 陈淑媛, 莫香, 等. 水稻耐冷相关基因克隆研究进展.生命科学研究, 2014, 18(2): 162-166. |
| [25] | Baruah A R, Ishigo-Oka N, Adachi M, et al .Cold tolerance at the early growth stage in wild and cultivated rice.Euphytica,2009, 165(3): 459-470. |
| [26] | Shimono H, Ishii A, Kanda E, et al.Genotypic variation in rice cold tolerance responses during reproductive growth as a function of water temperature during vegetative growth.Crop Sci, 2011, 51(1): 290-297. |
| [27] | Akihiro I, Takashi M, Yoshie H, et al.Spermidine synthase genes are essential for survival of Arobidopsis.Plant Physiol,2004, 135(9): 1565-1573. |
| [28] | Pillai M A, Akiyama T.Differential expression of an S-adenosyl-L-methionine decarboxylase gene involved in polyamine biosynthesis under low temperature stress in japonica and indica rice genotypes.Mol Gen Genom, 2004, 271: 141-149. |
| [29] | Lee M.DNA markers in plant breeding programs.Adv Agron, 1995, 55: 265-344. |
| [30] | 王军, 杨杰, 徐祥, 等. 水稻千粒重基因TGW6功能标记的开发与利用. 中国水稻科学, 2014, 28(5): 473-478. |
| [31] | Poczai P, Varga I, Laos M, et al.Advances in plant gene-targeted and functional markers: A review.Plant Methods, 2013, 9: 6. |
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