收稿日期: 2014-12-23
修回日期: 2015-01-05
网络出版日期: 2015-07-10
基金资助
中央级公益性科研院所专项基金资助项目(2012RG002-2);国家自然科学基金资助项目(31171532,31371606,31221004);浙江省“转基因农作物新品种培育”科技创新团队项目(2011R50021)
Subcellular Localization Analysis of Phosphatidylserine Synthase (OsSUI1) in Tobacco Mesophyll Cells
Received date: 2014-12-23
Revised date: 2015-01-05
Online published: 2015-07-10
赵娟, 康书静, 饶玉春, 邱振楠, 徐杰, 胡江, 张光恒, 曾大力, 郭龙彪, 钱前, 朱丽 . 水稻磷脂酰丝氨酸合酶SUI1在烟草叶肉细胞中的定位[J]. 中国水稻科学, 2015 , 29(4) : 343 -349 . DOI: 10．3969/j．issn．1001G7216．2015．04．002
SUI1 encodes a phosphatidylserine synthase. And its mutant sui1-1 exhibites a shortened uppermost internode and apartly sheathed panicle. On this basis, two vectors pMDC45-SOVT and pMDC201-SOV were constructed by the Gateway system, which responds to the N-terminal and C-terminal fusion GFP. To observe the subcellular localization of SUI1, tobacco leaves have been infected by Agrobacterium with destination vectors. The results showed that GFP-SUI1 fusion proteins positioned in the plasma membrane and nucleus, while SUI1-GFP were localized in the plasma membrane and the nuclear membrane. Moreover, the distribution of the two fusion proteins were not continuous in the plasma membrane. We speculate that SUI1 protein was localized in the plasma membrane and nuclear membrane of tobacco leaf cells because the target protein folding and transmembrane manner may be changed by the N-terminal fusion, affecting the correct position of the protein.
Key words： rice; SUI1; subcellular localization
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