收稿日期: 2015-01-05
修回日期: 2015-02-13
网络出版日期: 2015-05-10
基金资助
国家863计划资助项目(2012AA101102);国家超级稻育种专项(201401);中央级公益性科研院所基本科研业务费专项(2014RG003-1)
Dissection of Quantitative Trait Loci for Grain Weight and Its Component Traits in the qTGW1.2 Region on Chromosome 1 of Rice
Received date: 2015-01-05
Revised date: 2015-02-13
Online published: 2015-05-10
对水稻第1染色体长臂上控制粒重、粒长和粒宽的3个QTLs进行了剖析。从珍汕973/密阳46的BC2F8群体中筛选到1个在RM212-RM265区间呈杂合的单株,应用DNA标记检测其衍生的BC2F9群体,筛选出在RM212-RM11787和RM11787-RM265区间呈杂合的单株各1个,其杂合区间分别覆盖前期定位的千粒重QTL qTGW1.2a和qTGW1.2b。种植2个BC2F10群体,筛选出杂合区间相互交叠的单株各3个,自交后获得6个BC2F11群体,在RM11781-RM11800区间检测到了1个控制粒长的QTL,并将qTGW1.2a和qTGW1.2b分别缩小至580 kb的RM11730-Wn33304和2.0 Mb的RM11807-RM11885区间内。同时,筛选出5个杂合区间相互交叠的单株,衍生了5个BC2F12群体。QTL分析结果表明,每个群体均检测到粒长QTL,加性效应为0.03~0.06 mm,增效等位基因来自密阳46;经比较各个群体的分离区间,将控制粒长的QTL qGL1.2界定在RM11781和Wn34526之间372 kb的区间内;该QTL呈加性作用,可能同时控制粒重、粒长与粒宽。
关键词： 水稻(Oryza sativa L.); 数量性状座位; 粒长; 千粒重; 微效作用
王琳琳, 陈玉宇, 郭梁, 张宏伟, 樊叶杨, 庄杰云 . 水稻第1染色体qTGW1.2区域粒重组分性状QTL的剖析[J]. 中国水稻科学, 2015 , 29(3) : 232 -240 . DOI: 10．3969/j．issn．1001G7216．2015．03．002
This paper reports the dissection of three quantitative trait loci(QTLs)for 1000-grain weight (TGW),grain length(GL)and grain width (GW) on the long arm of chromosome 1 in rice. A plant carrying a heterozygous segment extending from RM212 to RM265 was selected from the Zhenshan 973/Milyang 46 BC2F8 population. The resulting BC2F9 population was assayed with DNA markers. Two plants were identified,carrying heterozygous segments covering the intervals RM212-RM11787 and RM11787-RM265 which were previously found to harbor qTGW1.2a and qTGW1.2b for TGW,respectively. Two BC2F10 populations were produced,from which three plants with sequential heterozygous regions were identified,respectively. Using the six BC2F11 populations derived,a QTL for GL was detected in the interval RM11730-RM11800, and the regions for qTGW1.2a and qTGW1.2b were narrowed down to the 580 kb interval RM11730-Wn33304 and 2.0 Mb interval RM11807-RM11885,respectively. In the meantime,five plants with smaller heterozygous regions were selected,from which five BC2F12 populations were constructed. A QTL for GL was detected in each of the five populations. The additive effect ranged from 0.03 mm to 0.06 mm with the enhancing alleles derived from Milyang 46. Based on comparison of the segregating regions among the five populations,qGL1.2 for GL was delimited to a 372 kb region flanked by RM11781 and Wn34526. This QTL had the additive genetic action mode and may simultaneously affect TGW, GL and GW.
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