
收稿日期: 2014-08-05
修回日期: 2014-10-20
网络出版日期: 2015-03-10
基金资助
浙江省杰出青年基金资助项目(LR12C06001)
Application of the Second Generation Sequencing Technology in Rice Genomics and Transcriptomics
Received date: 2014-08-05
Revised date: 2014-10-20
Online published: 2015-03-10
高阳, 薛大伟, 钱前, 高振宇 . 二代测序技术在水稻基因组学和转录组学研究中的应用[J]. 中国水稻科学, 2015 , 29(2) : 208 -214 . DOI: 10.3969/j.issn.1001-7216.2015.02.013
The second generation sequencing technology made a revolutionary breakthrough in the field of sequencing technology with advantages, including high throughput, high precision and low cost. It provided a new method for the research in rice genomics. Here, we reviewed the theory and development of the second generation sequencing technology including sequencing by synthesis and sequencing by ligation, and summarized its applications in genomics, involving structural genomics and functional genomics, and transcriptomics of rice. Finally, the prospects of current sequencing technologies and their applications in rice research were also discussed.
Key words: the second generation sequencing; rice; genomics; transcriptomics
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