收稿日期: 2014-03-02
修回日期: 2014-04-13
网络出版日期: 2015-01-10
基金资助
转基因生物新品种培育重大专项(2012ZX08009001);浙江省农业科学院省部共建国家重点实验室培育基地开放基金资助项目(2010DS700124-KF1210)
Improvement of the Micro-chamber Inoculation Method for Determination of Rice Seedling Resistance to Sheath Blight (Rhizoctonia solani)
Received date: 2014-03-02
Revised date: 2014-04-13
Online published: 2015-01-10
水稻对纹枯病的抗性属于典型的数量性状,多数栽培品种的抗病水平较低,并且抗性差异较小。水稻纹枯病接种和抗性评价体系是培育抗病品种的重要基础。利用植物生长箱的控温、控光和控湿条件以及生长势相对一致的水稻秧苗,对苗期纹枯病微室接种技术进行了改进。试验品种苗期的纹枯病抗性从高到低依次为YSBR1、特青、泰粳394、日本晴和Lemont,并且与大田成株期接种鉴定的结果一致。RT-PCR分析显示,苗期和成株期接种纹枯病菌均诱导4个水稻抗病相关基因的表达。通过“叶枕高”和“苗挺高”计算的各品种苗期病级变幅分别为2.82~8.54级和1.20~3.39级。前者与大田成株期的病级变幅一致,因而“叶枕高”病级计算方法更适用于微室接种鉴定体系。
关键词： 水稻; 纹枯病; Rhizoctonia solani; 微室法; 抗病相关基因
徐国娟, 袁正杰, 左示敏, 潘学彪, 王志龙, 瞿绍洪 . 水稻苗期纹枯病抗性鉴定微室接种技术的改良[J]. 中国水稻科学, 2015 , 29(1) : 97 -105 . DOI: 10.3969/j.issn.1001-7216.2015.01.012
The sheath blight resistance in rice belongs to a typical quantitative trait, and most of the rice cultivars show low levels of resistance to the disease with small differences. The inoculation and scoring system for rice sheath blight is an important basis for breeding resistant rice cultivars. The micro-chamber method for determination of rice responses to the sheath blight pathogen Rhizoctonia solani was improved by inoculation to rice seedlings with similar growth vigour under controlled illumination, temperature and humidity conditions in a plant growth chamber. The rice cultivars YSBR1, Teqing, Taijing 394, Nipponbare and Lemont showed high to low levels of sheath blight resistance , which was consistent with the results of field inoculation in adult rice plants. RT-PCR analysis showed that the expression of four rice genes related to disease resistance was induced in inoculated seedlings and adult plants. Based on the height of the pulvinus of the second leaf from the top and the straightened seedling height the sheath blight scores ranged from 2.82 to 8.54 and from 1.20 to 3.39, respectively. The scoring method based on the height of the pulvinus led to similar disease scores for both seedlings and adult plants, and thus is more suitable for the micro-chamber inoculation system.
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