稻瘟病菌大片段DNA转化载体的构建
收稿日期: 2014-03-28
修回日期: 2014-06-11
网络出版日期: 2015-01-10
基金资助
国家自然科学基金资助项目(30670072);浙江省自然科学基金资助项目(Y305068)
Construction of a Vector for Large DNA Fragment Transformation in Magnaporthe oryzae
Received date: 2014-03-28
Revised date: 2014-06-11
Online published: 2015-01-10
陈亚平, 施文骁, 王洪凯 . 稻瘟病菌大片段DNA转化载体的构建[J]. 中国水稻科学, 2015 , 29(1) : 91 -96 . DOI: 10.3969/j.issn.1001-7216.2015.01.011
It is an important means to study function of gene cluster using special vectors for large DNA fragment transformation. A vector was constructed for large DNA fragment transformation based on cosmid super Cos I vector and pBHt2 vector. More than 30 kb DNA fragment was transferred into the rice blast fungus successfully using Agrobacterium tumefaciens-mediated transformation. The results will promote functional analysis of gene cluster in Magnaporthe oryzae.
Key words： vector; transformation; large DNA fragment; Magnaporthe oryzae
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