水稻白条纹叶突变体 st11的遗传分析与基因定位
收稿日期: 2014-02-19
修回日期: 2014-08-15
网络出版日期: 2015-01-10
基金资助
国家自然科学基金资助项目(31171622);四川省“百人计划”资助项目;四川省青年基金资助项目(2013JQ0038)
Genetic Analysis and Gene Mapping of White Stripe Leaf Mutant st11 in Rice
Received date: 2014-02-19
Revised date: 2014-08-15
Online published: 2015-01-10
白条纹叶突变体 st11是从粳稻品种Kitaake组培过程中获得的。该突变体在分蘖前叶色表现为正常,从分蘖期开始新生叶表现为白条纹直至成熟期。与野生型相比,该突变体的分蘖、株高、结实率和千粒重等农艺性状没有发生明显变化。遗传分析表明该突变体白条纹叶性状受一对隐性核基因控制。利用该突变体分别与水稻02428、Jodan杂交构建了两个F2群体用于基因定位。通过集群分离分析(bulked segregant analysis)发现该基因位于第1染色体端粒附近,并与分子标记RM151和RM10080连锁。进一步利用更多分子标记分析F2群体,我们将该基因定位于I10和I26两个标记之间大约270kb的区间内。
成钦淑, 叶邦全, 袁灿, 李伟滔, 尹俊杰, 王静, 贺闽, 汪吉春, 王玉平, 李仕贵, 陈学伟 . 水稻白条纹叶突变体 st11的遗传分析与基因定位[J]. 中国水稻科学, 2015 , 29(1) : 14 -21 . DOI: 10.3969/j.issn.1001-7216.2015.01.002
A white stripe leaf mutant st11 was obtained from tissue-cultured rice Kitaake (Oryza sativa spp japonica). This mutant was characterized by white stripe leaves from tillering stage to mature stage. Compared with the wild type Kitaake, the mutant showed no obvious changes in agricultural traits, such as tiller number, plant height, seed-setting rate, and grain weight. Genetic analyses showed that the white stripe leaf phenotype in the mutant st11 was controlled by a single recessive gene. Two F2 segregating populations derived from the crosses, st11×Jodan and st11×02428, respectively, were used for mapping the gene st11. Bulked segregant analysis suggested that the gene st11 was located close to the markers, RM151 and RM10080, on chromosome 1. Using more molecular markers, we finally delimited the gene st11 to a region of about 270 kb between the Indel markers, I10 and I26, on the telomere of the short arm of chromosome 1.
Key words： rice; white stripe leaf mutant; molecular marker; gene mapping
| [1] | 金怡, 刘合芹, 汪得凯, 等. 一个水稻苗期白条纹叶及抽穗期白穗突变体的鉴定和基因定位. 中国水稻科学, 2011, 25(5): 461-466. |
| [2] | 张洪征, 程治军, 万建民, 等. 水稻白化突变体研究进展. 生物技术通报, 2013, 1(11): 1-7. |
| [3] | 施勇烽, 魏彦林, 奉保华, 等. 水稻淡绿叶突变体HM14的遗传分析与基因定位. 中国水稻科学, 2013, 27(6): 585-590. |
| [4] | Liu W, Fu Y, Hu G, et al.Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.).Planta, 2007, 226(3): 785-795. |
| [5] | 王平荣, 张帆涛, 高家旭, 等. 高等植物叶绿素合成的研究进展. 西北植物学报, 2009, 29(3): 629-636. |
| [6] | Jung K H, Hur J, Ryu C H, et al.Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system.Plant Cell Physiol, 2003, 44(5): 463-463. |
| [7] | Zhang H, Li J, Yoo J H, et al.Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase,a key enzyme for chlorophyll synthesis and chloroplast development.Plant Mol Biol, 2006, 62(3): 325-337. |
| [8] | Lee S, Kim J H, Yoo E S, et al.Differential regulation of chlorophyll a oxygenase genes in rice.Plant Mol Biol, 2005, 57(6): 805-818. |
| [9] | Wang P, Gao J, Wan C, et al.Divinyl chlorophyll(ide) a can be converted to monovinyl chlorophyll(ide) a by a divinyl reductase in rice.Plant Physiol, 2010, 153(3): 994-1003. |
| [10] | Wu Z, Zhang X, He B, et al.A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis.Plant Physiol, 2007, 145(1): 29-40. |
| [11] | Park S Y, Yu J W, Park J S, et al.The senescence-induced stay green protein regulates chlorophyll degradation.Plant Cell, 2007, 19(5): 1649-1664. |
| [12] | Kusaba M, Ito H, Morita R, et al.Rice NON-YELLOW COLOR COLORING1 is involved in light-harvesting complex II and grana degradation during leaf senescence.Plant Cell, 2007, 19(4): 1362-1375. |
| [13] | Sato Y, Morita R, Katsuma S, et al.Two short-chain dehydrogenasereductases, NON-YELLOW COLORING 1 and NYC1-LIKE are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice.Plant J, 2009, 57(1): 120-131. |
| [14] | Morita R, Sato Y, Masuda Y, et al.Defect in non-yellow coloring 3, an αβ hydrolase-fold family protein,causes a stay-green phenotype during leaf senescence in rice.Plant J, 2009, 59(6): 940-952. |
| [15] | Kusumi K, Sakata C, Nakamura T, et al.A plastid protein NUS1 is essential for build-up of the genetic system for early chloroplast development under cold stress conditions.Plant J, 2011, 68(6): 1039-1050. |
| [16] | Sugimoto H, Kusumi K, Tozawa Y, et al.The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation.Plant Cell Physiol, 2004, 45(8): 985-996. |
| [17] | Yoo S C, Cho S H, Sugimoto H, et al.Rice virescent 3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development.Plant Physiol, 2009, 150(1): 388-401. |
| [18] | Gothandam K M, Kim E S, Cho H, et al.OsPPR1, a pentatricopeptide repeat protein of rice is essential for the chloroplast biogenesis.Plant Mol Biol, 2005, 58(3): 421-433. |
| [19] | Rogers S O, Bendich A J.Extraction of DNA from plant tissues.Plant Mol Biol Manual, 1988, 6(1):1-10. |
| [20] | Michelmore R W, Paran I, Kesseli R V.Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations.Proc Natl Acad Sci USA, 1991, 88(21): 9828-9832. |
| [21] | 杨麟, 罗大刚. 水稻叶色突变体的研究进展. 安徽农业科学, 2013, 41(8): 3341-3342. |
| [22] | Nagata N, Tanaka R, Satoh S, et al.Identification of a vinyl reductase gene for chlorophyll synthesis in Arabidopsis thaliana and implications for the evolution of Prochlorococcus species.Plant Cell, 2005, 17(1): 233-240. |
| [23] | Maekawa M.Studies oil gen etieal difference between distantly related dee varieties.Memoirs Res Fac Agric Hokk Univ, 1982, 13(2): 146-177. |
| [24] | 刘少奎, 张启军, 漆庆明, 等. 水稻白条纹新基因st9(t)的初步定位. 江苏农业学报, 2012, 28(5): 928-923. |
| [25] | Nagao S.Genie analysis and linkage relationship of characters in rice.Adv Genet, 1951, 4(1): 181-211. |
| [26] | Maekawa M.A new leaf stripe genest-5, its linkage with d2 and the location of gene P in the second linkage group.Rice Genet Newsl, 1988, 5(1): 85-87. |
| [27] | Maekawa M, Inukai T, Shinbashi N.A new gene for leaf stripe (st-6) fund in linkage group 3.Rice Genet Newsl, 1990, 7(1): 108-109. |
| [28] | 谭振华. 水稻白条纹叶基因OsPPR3的图位克隆及功能分析. 长沙: 湖南大学, 2012. |
| [29] | 何颖红, 邹国兴, 饶玉春等. 水稻白条叶突变体(st10)的遗传分析与基因定位. 分子植物育种, 2011, 9(2): 136-142. |
| [30] | Su N, Hu M, Wu D, et al.Disruption of a rice pentatricopeptide repeat protein causes a seedling-specific albino phenotype and its utilization to enhance seed purity in hybrid rice production.Plant Physiol, 2012, 159(1): 227-238. |
| [31] | 曹立勇, 钱前, 朱旭东, 等. 紫叶标记籼型光温敏核不育系中紫S的选育及其配组的杂种优势. 作物学报, 1999, 25(1): 44-49. |
| [32] | Oud J S N, Schneiders H, Koo A J, et al. Breeding of transgenic orange Petunia hybrid varieties.Euphytica, 1995, 84(3): 175-181. |
/
| 〈 |
|
〉 |