以16个不同来源的云南野生稻作为供试材料，用抗稻瘟病Pita基因特异引物(KG2/KG4)进行PCR扩增，并对PCR产物进行克隆、测序及分析。结果表明，16个野生稻中7个品种持有抗稻瘟病Pita基因，其中，来自云南景洪的紫秆普通野生稻中Pita基因的编码区与原始型的抗稻瘟病Pita基因的DNA序列完全相同。可见，云南可能是抗稻瘟病基因Pita的起源地之一。
A total of 16 accessions of wild rice from different regions of Yunnan Province were used for PCR amplification with Pita gene specific primers. The amplified PCR products were cloned into Topo cloning vector, and sequenced. Among 16 accessions, the identical sequence fragment to the resitance Pita gene was amplified separately from 7 accessions. The Pita allele in one accession of Oryza rufipogon with purple stem from Jinghong contains an identical DNA sequence in the coding region to the original Pita resistance gene. It suggested that Yunnan might be one of the origins of the Pita gene.
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