常规稻与杂交稻不同生育期叶片挥发物分析

黄敏1 ,胡飞1,*,杨军2,姬静华1,陈雄辉1

doi:10.3969/j.issn.1001-7216.2014.06.010

中国水稻科学 >

2014 , Vol. 28 >Issue 6: 639 - 648

DOI: https://doi.org/10.3969/j.issn.1001-7216.2014.06.010

研究报告

常规稻与杂交稻不同生育期叶片挥发物分析

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¹华南农业大学农学院，广州 510642；²华南农业大学测试中心，广州510642；

收稿日期: 2014-05-23

修回日期: 2014-09-15

网络出版日期: 2014-11-10

基金资助

公益性行业（农业）科研专项（201403030）。

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Component Analysis on Volatile from Conventional and Hybrid Rice Leaves During Different Developmental Stages

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¹College of Agriculture, South China Agricultural University, Guangzhou 510642, China; ² Instrumental Analysis & Research Center, South China Agricultural University, Guangzhou 510642, China;

Received date: 2014-05-23

Revised date: 2014-09-15

Online published: 2014-11-10

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摘要

利用固相微萃取法（SPME）和气质联用（GCMS）技术对常规稻（化感稻3号、象牙香占）和杂交稻（软华优3号、农珍优3号）的4个不同生育期叶片挥发物成分及其相对含量进行了比较分析。结果表明，杂交稻叶片挥发物成分比常规水稻多。化感稻3号共检测出41种物质，象牙香占共检测到37种物质。软华优3号共检测出49种物质，农珍优3号共检测出53种物质。化感稻3号、象牙香占和软华优3号均是乳熟期挥发物成分最多，农珍优3号则是完熟期最多。在同一生育期常规稻和杂交稻叶片挥发物成分及相对含量虽然有一定差异，但规律不明显。随水稻的成熟叶片烷烃类和萜烯类的相对含量总体呈下降趋势。4种水稻各生育期共有的化合物为长叶烯和角鲨烯。软华优3号在扬花期检测出(Z)3己烯醇，象牙香占和农珍优3号在完熟期检测出γ己内酯两种绿叶挥发物。这些化合物与水稻品质、有害生物之间的关系值得进一步深入研究。

关键词： 常规稻; 杂交稻; 叶片挥发物; 固相微萃取法; 气质联用技术

本文引用格式

黄敏1 ,胡飞1,*,杨军2,姬静华1,陈雄辉1 . 常规稻与杂交稻不同生育期叶片挥发物分析[J]. 中国水稻科学, 2014 , 28(6) : 639 -648 . DOI: 10.3969/j.issn.1001-7216.2014.06.010

Abstract

Volatile components of conventional and hybrid rice leaves were analysed by solid phase microextraction (SPME) and gas chromatographymass spectrometry(GCMS) with two conventional rice varieties(Allelopathic rice 3, Xiangyaxiangzhan) and two hybrid rice combinations (Ruanhuayou 3, Nongzhenyou 3) as materials during four developmental stages. The results showed that 41 and 37 components were identified in Allelopathic rice 3 and Xiangyaxiangzhan; 49 and 53 in Ruanhuayou 3 and Nongzhenyou 3. For Allelopathic rice 3, Xiangyaxiangzhan and Ruanhuayou 3, the number of volatile components maximized during milkripe stage, while for Nongzhenyou 3 during fullripe stage. There were some insignificant differences in volatile components and their relative content between conventional rice and hybrid rice combination during the same stage. The relative content of alkanes and terpenes decreased with rice ripening in the four tested rice. Longifolene and Squalene were identified in all tested rice during different stages. (Z)3Hexenol from Ruanhuayou 3 in flowering stage and γCaprolactone from Xiangyaxiangzhan and Nongzhenyou 3 in fullripe stage were identified, these two components were green leaf volatiles.The relationship between these components and rice quality, pests was worth of further study.

Key words： conventional rice; hybrid rice; leaf volatile; SPME； GCMS

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