研究报告

水稻移栽后新生根系相关性状的QTL分析

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  • 黑龙江省农业科学院 耕作栽培研究所, 哈尔滨 150086;

收稿日期: 2014-01-10

  修回日期: 2014-03-03

  网络出版日期: 2014-11-10

基金资助

国家自然科学基金资助项目(31100881);中国博士后科学基金特别资助项目(2013T60396);中国博士后科学基金资助项目(2011M501077);黑龙江省博士后资助经费(LBHZ11030)。

QTL Analysis on New Root Traits after Rice Transplanting

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  • Cultivation and Farming Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086; 

Received date: 2014-01-10

  Revised date: 2014-03-03

  Online published: 2014-11-10

摘要

为揭示水稻移栽后新生根系相关特性的遗传基础,以笹锦、北陆129及其杂交衍生的回交重组自交系群体为材料,构建包含对应的由245个分子标记组成的遗传图谱对水稻移栽后的新生根相关性状进行分析。共检测到10个控制根平均直径、总根长、平均根表面积、平均根长和根数等性状的QTL。这些QTL分布在第1、2、5、9和11染色体上,并在第9和11染色体上成簇分布,表型贡献率为10.7%~28.5%。随后利用“选择作图”策略对主效QTL进行了验证分析。其中,位于第11染色体上标记C477和G320B之间(5 461 121-6 686 166 bp)的同时控制根长、根表面积和根数的QTL qRL11.1,qASA11和qRN11.1是新发现的位点,具有一定的研究与应用价值。

本文引用格式

姜树坤,张凤鸣* ,白良明,孙世臣,王彤彤,丁国华,姜辉,张喜娟 . 水稻移栽后新生根系相关性状的QTL分析[J]. 中国水稻科学, 2014 , 28(6) : 598 -604 . DOI: 10.3969/j.issn.1001-7216.2014.06.005

Abstract

To reveal the genetic basis of the new root  traits after transplanting, by using Sasanishiki, Habataki and 85 backcross recombinant inbred lines (BC1F5) and its linkage map including 245 molecular markers QTL controlling new root   traits after transplanting were detected. Ten QTLs controlling average diameter, total root length, average surface area, root length and root number were identified. These QTLs distributed on chromosome 1, 2, 5, 9 and 11. The phenotypic variations (VE) explained by individual QTL ranged from 10.7% to 28.5%. QTL clusters were formed on chromosome 9 and 11. Then four major QTLs were fine mapped by “selective mapping” method. The QTLs (qRL11.1,qASA11 and qRN11.1) for root length, average surface area and root number were located between C477 and G320B (5 461 121-6 686 166 bp) on chromosome 11. It was a novel locus with research and application value. 

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