研究报告

利用大粒籼/小粒粳重组自交系定位水稻生育期及产量相关性状QTL

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  • 中国水稻研究所 国家水稻改良中心/浙江省超级稻研究重点实验室, 杭州 310006;

收稿日期: 2013-12-30

  修回日期: 2014-02-10

  网络出版日期: 2014-11-10

基金资助

浙江省超级稻研究重点实验室(2013E10021);国家自然科学基金资助项目(31101209,31221004);浙江省8812计划资助项目(2012C129011)。

QTL Mapping of Heading Date and Yieldrelated Traits in Rice Using a Recombination Inbred Lines (RILs) Population Derived from BG1/XLJ

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  • National Center for Rice Improvement/Zhejiang  Key Laboratory of  Super Rice Research,China National Rice Research Institute, Hangzhou 310006, China;

Received date: 2013-12-30

  Revised date: 2014-02-10

  Online published: 2014-11-10

摘要

生育期及产量相关性状如株高、单株有效穗数、穗长、每穗实粒数、每穗总粒数和结实率等是水稻重要的农艺性状,发掘新的生育期及产量相关性状QTL,对克隆控制水稻产量性状新基因及利用分子聚合育种改良水稻产量性状具有重要意义。以两个在生育期及穗部性状上具有明显差异的亲本(BG1和XLJ)衍生的重组自交系群体为材料,在杭州(2011年)和海南(2012年)两个环境条件下,检测了影响水稻生育期及穗部相关性状的QTL,并对QTL上位性效应及与环境的互作进行了分析。共检测出影响生育期及产量相关性状的32个QTL,分布于第2、3、4、5、6、7和8染色体上,此外,分别检出5对双因子互作和5个QTL与环境互作。检测到的影响产量性状的QTL与前人研究结果相近的区间,其中效应较大、在不同环境下能被稳定检测到的QTL大都表现为成簇分布;重要产量性状QTL的上位性及与环境的互作效应均较小,说明基因间及基因与环境间的互作较小。

本文引用格式

占小登,于萍,林泽川,陈代波,沈希宏,张迎信,付君林,程式华* ,曹立勇* . 利用大粒籼/小粒粳重组自交系定位水稻生育期及产量相关性状QTL[J]. 中国水稻科学, 2014 , 28(6) : 570 -580 . DOI: 10.3969/j.issn.1001-7216.2014.06.002

Abstract

Heading date and yieldrelated traits including plant height (PH), number of tillers per plant (NTP), panicle length (PL), number of filled grains per panicle (NFGP), grain number per panicle (GNPP) and seed setting rate (SR) are important agronomic traits in rice. Detection of new QTL associated with heading date and yieldrelated traits have great significance in new genes identification and utilization of rice. We used a recombination inbred lines (RILs) population derived from BG1/XLJ to detect QTL associated with heading date and yieldrelated traits in two environments, effects of epistatic and interaction with environments were also analyzed. A total of 32 QTL associated with heading date and yield traits were detected on chromosomes 2, 3, 4, 5, 6, 7 and 8. Furthermore, five pairs of QTL intervals had significant twofactor epistatic effects, and 5 QTL had significant interaction with environments. Many yield related QTL detected were identified near the intervals where yieldrelated QTL were detected in previous researches. Among them, the QTL with large effects commonly clustered. Low epistatic and interaction with environments effects for yield related QTL, indicated small interactive effects between genes, genes and environments. 

参考文献

\[1\]程式华. 中国超级稻育种. 北京: 科学出版社, 2010: 1.

\[2\]Normile D. Reinventing rice to feed the world. Science, 2008, 321: 330333.

\[3\]邓启云, 袁隆平, 邓化冰, 等. 野生稻高产基因分子标记辅助育种研究进展//湖南省首届农业生物技术发展论坛论文集. 长沙, 2004: 8082.

\[4\]Jiao Y Q, Wang Y H, Xue D W, et al. Regulation of OsSPL14 by OsmiR156  defines ideal plant architecture in rice. Nat Genet, 2010, 42(6): 541544.

\[5\]Miura K,  Ikeda M,  Matsubara A, et al. OsSPL14 promotes panicle branching and higher grain productivity in rice. Nat Genet, 2010, 42(6): 545549.

\[6\]Li X Y, Qian Q,  Fu Z M, et al. Control of tillering in rice. Nature, 2003, 422(6932): 618621.

\[7\]Wei X J,  Xu J F, Guo H N, et al. DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously. Plant Physiol, 2010, 153(4): 17471758.

\[8\]Xue W Y, Xing Y Z, Weng X H, et al. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet, 2008, 40(6): 761767.

\[9\]Wu W X,  Zheng X M, Lu G W, et al. Association of functional nucleotide polymorphisms at  DTH2 with the northward expansion of rice cultivation in Asia. Proc Natl Acad Sci, 2013, 110(8): 27752780.

\[10\]Yoshida A,  Sasao M,  Yasuno N, et al.  TAWAWA1, a regulator of rice inflorescence architecture, functions through the suppression of meristem phase transition.  Proc Natl Acad Sci, 2013, 110(2): 767772.

\[11\]Qiao Y L, Piao R H,  Shi J X, et al. Fine mapping and candidate gene analysis of dense and erect panicle 3,  DEP3,  which confers high grain yield in rice (Oryza sativa L.). Theor Appl Genet, 2011, 122(7): 14391449.

\[12\]Huang X Z, Qian Q, Liu Z B,  et al. Natural variation at the  DEP1 locus enhances grain yield in rice. Nat Genet, 2009, 41(4): 494497.

\[13\]Moncada P, Martinez C P, Borrero J, et al. Quantitative trait loci for yield and yield components in an Oryza sativaOryza rufipogon BC2F2 population evaluated in an upland environment. Theor Appl Genet, 2001, 102: 4152.

\[14\]Orjuela J, Garavito A, Bouniol M,  et al. A universal core genetic map for rice. Theor Appl Genet, 2010, 120: 563572.

\[15\]van Ooijen J W. JoinMap 4,Software for the calculation of genetic linkage maps in experimental populations.  Wageningen, Netherlands: Kyazma BV, 2006.

\[16\]Wang S, Basten C J,Zeng Z B. Windows QTL Cartographer 25 department of statistics. Raleigh, USA: North Carolina State University, 2006.

\[17\]McCouch S R. Gene nomenclature system for rice. Rice, 2008, 1: 7284.

\[18\]Yang J, Hu C, Hu H,  et al. QTL Network: Mapping and visualizing genetic architecture of complex traits in experimental populations. Bioinformatics, 2008, 24: 721723.

\[21\]Wang R X, Hai L, Zhang X Y, et al. QTL mapping for grain filling rate and yieldrelated traits in RILs of the Chinese winter wheat population Heshangmai×Yu 8679. Theor Appl Genet, 2009, 118(2): 313325.

\[20\]Li J M, Thomoson M, McCouch S R. Fine mapping of a grainweight quantitative trait locus in the pericentromeric region of rice chromosome 3. Genetics, 2004, 168: 21872195.

\[21\]Miyako U T, Yukiko F, Masatomo K, et al. Rice dwarf mutant d1,which is defective in the α subunit of the heterotrimeric G protein,affects gibberellin signal transduction. Proc Natl Acad Sci USA, 2000, 97(21): 1163811643.

\[22\]Kazumitsu O, Horiuchi Y, Noriko I, et al. A QTL cluster for plant architecture and its ecological significance in Asian wild rice. Breeding Sci, 2007, 57(1): 716.

\[23\]Liu G F, Jian Y, Xu H M, et al. Influence of epistastasis and QTL×environment interaction on heading date of rice(Oryza sativa L.). J Gene Genom, 2007, 34(7): 608615.

\[24\]Gao Y M, Zhu J. Mapping QTLs with digenic epistasis undermultiple environments and predicting heterosis based on QTL effects. Theor Appl Genet, 2007, 115(3): 325333.
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